Detailed information NTG0020284

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: Spy49_1262c
• Genome accession: CP000829
• Coordinates: 1267611..1269995 (-)
• Length: 794 a.a.
• NCBI ID: ACI61546.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes NZ131
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1262611..1274995

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Spy49_1257c	pknB	1262629..1264527 (-)	1899	ACI61542.1	Serine/threonine protein kinase	Regulator
Spy49_1259c	pppL	1264524..1265264 (-)	741	ACI61543.1	Putative phosphoprotein phosphatase (pppL protein)	-
Spy49_1260c	sunL	1265302..1266624 (-)	1323	ACI61544.1	Putative RNA-binding Sun protein	-
Spy49_1261c	fmt	1266614..1267549 (-)	936	ACI61545.1	Methionyl-tRNA formyltransferase	-
Spy49_1262c	priA	1267611..1269995 (-)	2385	ACI61546.1	Arrested fork binding	Machinery gene
Spy49_1263c	-	1270060..1270377 (-)	318	ACI61547.1	DNA-directed RNA polymerase omega subunit	-
Spy49_1265c	-	1270393..1271028 (-)	636	ACI61548.1	Guanylate kinase	-
Spy49_1266c	-	1271138..1272745 (-)	1608	ACI61549.1	hypothetical protein	-
Spy49_1267c	-	1272875..1273771 (-)	897	ACI61550.1	Putative transcriptional regulator	-

Sequence

Protein

• Length: 794 a.a.
• Molecular weight: 90397.39 Da
• Isoelectric Point: 9.4590

>NTDB_id=20284 Spy49_1262c ACI61546.1 1267611..1269995(-) (priA) [Streptococcus pyogenes NZ131]
MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGKGNRLLQGFVIGFGQEDSSSLKLIQTVLDPEPVLNQE
QLTLADQLRKTVFSYKITLLKAMIPNLLNSNYDKVLRPESGLKKPDRDFLFEGKPSVLYSTLDREKEKIALKAIQAGHIT
VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQAFSRDVVAYFVTNHLIRIDERQI
DRSEPYFDQIKPSSFLTLNEQQASAVTEIVEQIGKPSKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQ
MTSRFISRFGKQVAIMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESNPRYHAREV
ALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEVTIVDFRDYIGQQAVSNFTPYLIDKIKERLV
KKEQVVLMLNRRGYSSFVMCRDCGYVDKCPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFD
ELQGVIPEARILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRASEKTFQ
LLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKKQDFEAFYRYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQV
TELLKSHLSDNIKILGPTPKPIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL

Nucleotide

• Length: 2385 bp

>NTDB_id=20284 Spy49_1262c ACI61546.1 1267611..1269995(-) (priA) [Streptococcus pyogenes NZ131]
ATGGAAAAAGTAGCTCATGTGATTGTAGATATTCCATTGATGCAAACGGACAAGCCATTTTCTTATGGTATTCCAAAAGA
GTTAGTTTCTCTCGTGCAGCTTGGTTCTCGTGTGCATGTTCCTTTTGGAAAAGGGAATCGTTTGTTGCAAGGCTTTGTTA
TTGGTTTTGGCCAGGAAGATTCAAGCTCTCTTAAACTGATTCAGACCGTTTTAGATCCTGAGCCAGTCCTAAACCAAGAA
CAATTGACCCTTGCTGATCAGCTTCGAAAAACAGTTTTTTCTTATAAAATCACGCTCTTAAAAGCAATGATTCCTAATCT
TCTTAACTCTAACTATGATAAAGTGCTAAGGCCAGAGTCAGGTCTAAAAAAGCCTGATAGAGACTTTCTTTTTGAAGGAA
AACCATCTGTCTTATATTCTACCTTAGACCGCGAGAAGGAAAAAATAGCTCTTAAGGCCATACAAGCTGGGCATATTACT
GTTAGTTATTTAGCCAAAGACAAGAAAAACCTAAAGACAGAAAAATATTATCACGTCGACTTAGACGCCTTAGCGGTACA
TCCCATCTCTAGTCGTGCCAAAAAGCGTCAATTATTAAAAGACTATCTGCTAACCCATACGAAGGAAGCTAAACTTGCAA
CGCTTTATCAAGCCTTTTCTCGAGATGTGGTGGCTTACTTTGTGACAAATCACTTAATTCGTATTGACGAACGTCAAATT
GATCGCAGTGAGCCCTATTTTGACCAGATCAAACCATCTTCTTTTTTAACCTTAAATGAACAGCAAGCGTCAGCTGTTAC
TGAAATTGTTGAGCAAATTGGGAAACCGAGTAAACCTTTTTTGATTGAAGGGATTACAGGTTCGGGTAAGACAGAAGTTT
ATCTTCATATCATCGAAGCAGTTTTAAAACAGGATAAGACAGCCATTGTTTTAGTACCAGAAATTTCGTTGACTCCTCAG
ATGACCTCTCGTTTTATTTCCCGTTTCGGAAAGCAAGTGGCTATTATGCATTCAGGCTTATCAGATGGTGAAAAGTTTGA
TGAGTGGCGCAAGATAAAAACAGGACAGGCTAAGGTTGTGGTTGGTGCCCGCTCTGCTATTTTCTCCCCACTAGAGCGGA
TTGGTGCAATTATTATTGATGAGGAGCATGAATCAACCTATAAACAAGAAAGCAACCCTAGATACCATGCCAGAGAGGTT
GCCCTGCTCAGAGCTAAACACCATCAAGCAGTTGTGGTTATGGGCTCTGCTACGCCAAGTATTGAAAGCCGAGCAAGGGC
TAGTAAGGGGGTCTATCATTTTATTCAATTAACGCAAAGAGCAAATCCTCTTGCTAAGATTCCAGAAGTAACGATTGTTG
ATTTTCGTGATTATATTGGACAGCAAGCAGTTAGCAATTTTACCCCATATTTGATTGATAAGATTAAAGAACGTTTGGTT
AAAAAAGAACAAGTTGTCTTAATGTTAAATAGACGTGGTTATTCTAGTTTTGTGATGTGTCGGGACTGTGGTTATGTGGA
CAAATGTCCCAACTGTGATATCTCCTTAACCCTTCACATGGATACTAAAACTATGAATTGCCATTACTGTGGGTTTCAAA
AACCTATCCCAATTACTTGTCCTGAGTGTCATAGCAACAGTATCCGCTATTATGGCACTGGAACTCAAAAAGCTTTTGAT
GAATTGCAAGGGGTTATCCCAGAAGCTAGGATCTTGAGAATGGATGTTGACACCACACGAAAAAAAAGAAGTCACAAAAC
CATACTAGATAGTTTTGGTCGCCAGGAAGCAGATATTTTGTTAGGGACGCAAATGATTGCCAAAGGACTGGATTTTCCAA
ATGTGACTTTAGTTGGGGTACTAAATGCAGATACCTCCTTAAATTTGCCAGATTTTCGAGCGTCAGAAAAAACGTTTCAA
TTGTTAACACAGGTTGCTGGTCGGGCAGGCCGTGCCCATAAGCCAGGAGAAGTCTTGATTCAAACTTATAATCCCGATCA
CTATGCTATTCAATTAGCTAAGAAACAAGATTTTGAAGCCTTTTACCGTTATGAAATGGCGATTCGCCGTCAGATGGCTT
ATCCCCCTTACTACTTTACAGTGGGTATTACGCTTTCGCATCGTTTAGAAGCATCGGTTGTAAAAAAAGCTTATCAAGTT
ACAGAACTGCTAAAGAGTCACTTATCGGATAATATTAAAATATTAGGACCGACTCCTAAGCCAATTGCTAGAACACACAA
TTTATACCACTATCAAATTTTGTTAAAATACCGTTTTGAAGATAACCTAGAAGAGACGCTCAATCGTATTCTTGATTGGA
GTCAAGAAGCAGATAATCGTCATTTAAAACTAATTATTGATTGTGAACCGCAACAATTTTTATAA

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-105)

• Primosomal protein N C-terminal domain (PriA_C) (696-790)

• Helicase conserved C-terminal domain (Helicase_C) (568-652)

• Type III restriction enzyme, res subunit (ResIII) (257-420)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (509-535)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	47.395	100	0.481