Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BJG91_RS17925 Genome accession   NZ_CP017577
Coordinates   2984569..2985612 (-) Length   347 a.a.
NCBI ID   WP_001013168.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain SCG04-02     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2979569..2990612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJG91_RS32215 - 2979634..2979738 (+) 105 Protein_3043 IS3 family transposase -
  BJG91_RS17880 (BJG91_17305) - 2980002..2980202 (-) 201 WP_000106083.1 YqzE family protein -
  BJG91_RS17885 (BJG91_17310) - 2980241..2980738 (-) 498 WP_065211832.1 shikimate kinase -
  BJG91_RS17890 (BJG91_17315) - 2980854..2981504 (-) 651 WP_000183857.1 2OG-Fe(II) oxygenase -
  BJG91_RS17895 (BJG91_17320) comGG 2981679..2982050 (-) 372 WP_001231317.1 competence type IV pilus minor pilin ComGG -
  BJG91_RS17900 (BJG91_17325) comGF 2982047..2982517 (-) 471 WP_000923101.1 competence type IV pilus minor pilin ComGF -
  BJG91_RS17905 (BJG91_17330) - 2982487..2982789 (-) 303 WP_000829469.1 hypothetical protein -
  BJG91_RS17910 (BJG91_17335) comGD 2982782..2983237 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  BJG91_RS17915 (BJG91_17340) comGC 2983234..2983533 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  BJG91_RS17920 (BJG91_17345) comGB 2983545..2984576 (-) 1032 WP_000475187.1 competence type IV pilus assembly protein ComGB -
  BJG91_RS17925 (BJG91_17350) comGA 2984569..2985612 (-) 1044 WP_001013168.1 competence type IV pilus ATPase ComGA Machinery gene
  BJG91_RS17930 (BJG91_17355) - 2985818..2986513 (+) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  BJG91_RS17935 (BJG91_17360) - 2986639..2986881 (+) 243 WP_000440716.1 DUF2626 domain-containing protein -
  BJG91_RS17940 (BJG91_17365) - 2986989..2988383 (+) 1395 WP_001094345.1 L-cystine transporter -
  BJG91_RS17945 (BJG91_17370) - 2988462..2988854 (-) 393 WP_065211831.1 hypothetical protein -
  BJG91_RS17950 (BJG91_17375) - 2988942..2989157 (-) 216 WP_001008327.1 DUF3912 family protein -
  BJG91_RS17955 (BJG91_17380) - 2989410..2989721 (+) 312 WP_001093237.1 hypothetical protein -
  BJG91_RS17960 (BJG91_17385) - 2989756..2990244 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39095.50 Da        Isoelectric Point: 8.7212

>NTDB_id=200504 BJG91_RS17925 WP_001013168.1 2984569..2985612(-) (comGA) [Bacillus thuringiensis strain SCG04-02]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVIQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEAGGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=200504 BJG91_RS17925 WP_001013168.1 2984569..2985612(-) (comGA) [Bacillus thuringiensis strain SCG04-02]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTTCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAATTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGCGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTTGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTAATACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACACTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGCTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCTAGCATATATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCTGGTGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.62

100

0.556
  pilB Haemophilus influenzae 86-028NP

40.373

92.795

0.375

Multiple sequence alignment