Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BG564_RS02700 Genome accession   NZ_CP017517
Coordinates   536677..537231 (+) Length   184 a.a.
NCBI ID   WP_006251773.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 22650     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 531677..542231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG564_RS02670 (BG564_02645) - 531896..532705 (+) 810 WP_006250123.1 metal ABC transporter permease -
  BG564_RS02675 (BG564_02650) - 532762..533505 (-) 744 WP_006250122.1 metal ABC transporter ATP-binding protein -
  BG564_RS02680 (BG564_02655) - 533518..534414 (-) 897 WP_006251769.1 metal ABC transporter substrate-binding protein -
  BG564_RS02685 (BG564_02660) - 534674..535834 (+) 1161 WP_020831129.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  BG564_RS02690 (BG564_02665) - 535875..536222 (-) 348 WP_006251771.1 helix-turn-helix domain-containing protein -
  BG564_RS02695 (BG564_02670) - 536206..536457 (-) 252 WP_006251772.1 type II toxin-antitoxin system RelE family toxin -
  BG564_RS02700 (BG564_02675) pilF 536677..537231 (+) 555 WP_006251773.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  BG564_RS02705 (BG564_02680) - 537231..539555 (+) 2325 WP_006251774.1 Tex family protein -
  BG564_RS02710 (BG564_02685) - 539626..540189 (-) 564 WP_142777988.1 nitroreductase family protein -
  BG564_RS02715 (BG564_02690) sppA 540297..542153 (+) 1857 WP_006249718.1 signal peptide peptidase SppA -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 20976.90 Da        Isoelectric Point: 7.0805

>NTDB_id=199502 BG564_RS02700 WP_006251773.1 536677..537231(+) (pilF) [Mannheimia haemolytica strain 22650]
MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLA
YYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
QKIAETLTELEKLDKPRANLLKEN

Nucleotide


Download         Length: 555 bp        

>NTDB_id=199502 BG564_RS02700 WP_006251773.1 536677..537231(+) (pilF) [Mannheimia haemolytica strain 22650]
ATGTTTGCAAAATTTTTTCCAAAAATGACCGCTTGTATTCTGATTTTTTTATTAGCGGCTTGTTCTTCTCTTCCCCCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTTTAGCTTATCTGGAGCAAAATG
ATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAAAAAGATTACCTCCCTTATTCGGTGCTTGCC
TATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCTTACCAAACGGCACTGAAATTGAGTCAAAACCGCCC
TGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGCAGGCTG
TCAAAAGCAAAGCACCCTACTATCACCAAGCGGATAGCTTAGAAAATATGGTGTTATGTGCGAAAAAAGAGCCGAATCTG
CAAAAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(95-126)

Tetratricopeptide repeat (TPR_8)

(40-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

63.636

100

0.647
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.953

92.935

0.446

Multiple sequence alignment