Detailed information NTG0198367

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BIZ52_RS07340
• Genome accession: NZ_CP017457
• Coordinates: 1635422..1636285 (+)
• Length: 287 a.a.
• NCBI ID: WP_027227023.1
• Uniprot ID: A0A2S6EZM3
• Organism: Legionella pneumophila subsp. fraseri strain Detroit-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1630422..1641285

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BIZ52_RS07320 (BIZ52_07235)	-	1630861..1631187 (-)	327	WP_027227020.1	DUF1820 family protein	-
BIZ52_RS07325 (BIZ52_07240)	-	1631370..1632113 (-)	744	WP_027227021.1	methyltransferase domain-containing protein	-
BIZ52_RS07330 (BIZ52_07245)	pilB	1632426..1634150 (+)	1725	WP_027227022.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BIZ52_RS07335 (BIZ52_07250)	pilC	1634156..1635376 (+)	1221	WP_027221710.1	type II secretion system F family protein	Machinery gene
BIZ52_RS07340 (BIZ52_07255)	pilD	1635422..1636285 (+)	864	WP_027227023.1	A24 family peptidase	Machinery gene
BIZ52_RS07345 (BIZ52_07260)	-	1636299..1637504 (-)	1206	WP_027227024.1	cyclic nucleotide-binding domain-containing protein	-
BIZ52_RS07350 (BIZ52_07265)	-	1637614..1638207 (-)	594	WP_027227025.1	hypothetical protein	-
BIZ52_RS07355 (BIZ52_07270)	-	1638223..1638678 (-)	456	WP_027227026.1	hypothetical protein	-
BIZ52_RS07360 (BIZ52_07275)	-	1638826..1639641 (+)	816	WP_027227027.1	pyruvate, water dikinase regulatory protein	-
BIZ52_RS07365 (BIZ52_07280)	-	1639723..1641177 (-)	1455	WP_027227028.1	bifunctional 2-methylcitrate dehydratase/aconitate hydratase	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 32867.63 Da
• Isoelectric Point: 8.2759

>NTDB_id=198367 BIZ52_RS07340 WP_027227023.1 1635422..1636285(+) (pilD) [Legionella pneumophila subsp. fraseri strain Detroit-1]
MINTLIINYTWFMYLVIGLFSLAVGSLLNVIIYRLPIILQEEWREQCCELLNLEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCKSPISIRYPLIELLTTVLSLYASWHFGFTVQLLFVLLAIWILIPLTFIDLDHQLLPDSLTL
GLLWLGLIANTWSIFVSLDIAVLSCAGAYLALWLFINLFYLITGKVGMGHGDFKLFAAFGAWFGWMYLPLILLMSSITGA
IIGLIYLRINGKSRDTAIPFGPFLCISGLIAMFLGDSIINWYIGYWM

Nucleotide

• Length: 864 bp

>NTDB_id=198367 BIZ52_RS07340 WP_027227023.1 1635422..1636285(+) (pilD) [Legionella pneumophila subsp. fraseri strain Detroit-1]
ATGATAAATACGCTAATAATCAATTATACCTGGTTTATGTATTTAGTCATCGGACTCTTTTCTTTGGCCGTTGGAAGTTT
ACTTAATGTTATCATCTATCGCTTACCAATTATTTTGCAGGAAGAATGGCGAGAACAATGCTGTGAGTTACTTAATCTTG
AACAGCGAAAAGAAAAAATAAAACTTAATCTATTTTTACCACGTTCATTTTGCCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCCCTATTAAGCTATATCTTGTTAAGAGGTCGCTGTTACCAATGCAAAAGTCCCATTTCAATTCGCTA
TCCCCTTATTGAGTTATTGACCACAGTGTTATCATTATACGCCAGTTGGCACTTTGGATTTACAGTACAATTATTATTTG
TTTTATTAGCCATCTGGATATTGATACCTCTTACTTTTATTGATCTTGATCATCAATTACTCCCTGATAGTCTCACTTTA
GGACTATTATGGCTTGGTCTTATTGCAAATACATGGAGTATATTTGTTAGTCTTGATATTGCTGTGTTAAGTTGTGCCGG
TGCCTACTTAGCCTTATGGTTATTCATTAATCTATTTTATCTGATTACAGGTAAGGTAGGCATGGGGCATGGTGATTTCA
AATTATTTGCAGCATTTGGCGCATGGTTTGGCTGGATGTATTTACCATTAATTCTTCTGATGTCTTCAATAACCGGAGCT
ATCATTGGTTTGATTTATTTAAGGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGTCCTTTTCTTTGCATTTC
CGGGTTAATAGCTATGTTTTTGGGGGACTCCATTATCAATTGGTATATAGGTTATTGGATGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

3D structure

• AlphaFold DB: A0A2S6EZM3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.534	97.909	0.495
pilD	Acinetobacter nosocomialis M2	49.084	95.122	0.467
pilD	Acinetobacter baumannii D1279779	48.364	95.819	0.463
pilD	Vibrio campbellii strain DS40M4	46.403	96.864	0.449
pilD	Neisseria gonorrhoeae MS11	46.043	96.864	0.446