Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BI380_RS27680 Genome accession   NZ_CP017420
Coordinates   6061424..6063166 (-) Length   580 a.a.
NCBI ID   WP_034364744.1    Uniprot ID   A0AAX3SVK0
Organism   Delftia tsuruhatensis strain CM13     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6056424..6068166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BI380_RS27650 (BI380_27650) - 6057006..6057512 (+) 507 WP_016445605.1 (deoxy)nucleoside triphosphate pyrophosphohydrolase -
  BI380_RS27655 (BI380_27655) - 6057523..6057777 (-) 255 WP_016451023.1 DNA gyrase inhibitor YacG -
  BI380_RS27660 (BI380_27660) zapD 6057777..6058532 (-) 756 WP_026062416.1 cell division protein ZapD -
  BI380_RS27665 (BI380_27665) coaE 6058622..6059260 (-) 639 WP_016445608.1 dephospho-CoA kinase -
  BI380_RS27670 (BI380_27670) pilD 6059275..6060168 (-) 894 WP_016445609.1 prepilin peptidase Machinery gene
  BI380_RS27675 (BI380_27675) pilC 6060168..6061385 (-) 1218 WP_016445610.1 type II secretion system F family protein Machinery gene
  BI380_RS27680 (BI380_27680) pilB 6061424..6063166 (-) 1743 WP_034364744.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BI380_RS27690 (BI380_27690) - 6063403..6064332 (-) 930 WP_046238483.1 polyprenyl synthetase family protein -
  BI380_RS27695 (BI380_27695) rplU 6064563..6064874 (+) 312 WP_012207284.1 50S ribosomal protein L21 -
  BI380_RS27700 (BI380_27700) rpmA 6064890..6065147 (+) 258 WP_012207283.1 50S ribosomal protein L27 -
  BI380_RS27705 (BI380_27705) cgtA 6065297..6066412 (+) 1116 WP_016451026.1 Obg family GTPase CgtA -
  BI380_RS27710 (BI380_27710) proB 6066496..6067644 (+) 1149 WP_016445651.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62761.81 Da        Isoelectric Point: 5.1481

>NTDB_id=198019 BI380_RS27680 WP_034364744.1 6061424..6063166(-) (pilB) [Delftia tsuruhatensis strain CM13]
MAVADSSLKNASAVASLPGIGRALISAGKVSQQAAEAAAGKAATNRTSFIAELSVAGTLSALEVAETVSTMFSAPLLDLD
AVDAMRLPQDLLDAKIISTYRVLPLSKRGNRLTVATADPTQQEAAEQIKFTTQLGVDWIIVEADKLERLIAQQNKSASEA
MDSYGGSGDFEFGDLKPDEVAEEKDNSAAADVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIAS
PPIAIKEKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARMGIDALGYEPEE
KARLLEAISRPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKEPAD
IPREALIDAGYDDADIDGSWVTYRPVGCPACNNGYKGRVGIYQVMPISEEIQRIILQDGSALEIAAQAKREGVRSLRGSG
LHKARQGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=198019 BI380_RS27680 WP_034364744.1 6061424..6063166(-) (pilB) [Delftia tsuruhatensis strain CM13]
ATGGCTGTTGCTGACTCCTCGCTCAAGAATGCATCCGCTGTCGCATCGCTTCCCGGCATCGGGCGGGCTCTGATTTCAGC
AGGCAAGGTATCGCAACAGGCAGCGGAAGCGGCTGCCGGCAAGGCCGCCACCAATCGCACCTCGTTCATTGCAGAACTGA
GCGTCGCCGGCACCCTCTCTGCCCTGGAAGTGGCGGAGACGGTTTCCACCATGTTCAGCGCGCCGCTGCTCGATCTTGAT
GCGGTCGACGCCATGCGCCTGCCGCAGGATCTGCTGGACGCCAAGATCATCAGCACCTATCGCGTCCTGCCTCTGAGCAA
GCGCGGCAACCGCCTCACGGTGGCCACCGCCGACCCGACGCAGCAGGAAGCCGCCGAGCAGATCAAGTTCACCACCCAGT
TGGGCGTGGACTGGATCATCGTCGAAGCCGACAAGCTGGAGCGACTGATCGCGCAGCAGAACAAAAGCGCCAGCGAGGCC
ATGGACAGCTATGGCGGCAGCGGCGACTTCGAGTTCGGCGACCTCAAGCCTGATGAGGTGGCAGAGGAAAAGGACAACTC
GGCCGCAGCAGACGTCGAAGATGCGCCCATCGTCAAGTTCCTGCACAAAATGCTGCTCGACGCCTTCAACATGCGCGCGT
CTGACTTGCATTTCGAACCCTACGAGCACCAGTACCGCGTGCGCTTTCGCATTGACGGCGAGCTGCGTGAAATTGCATCG
CCGCCCATTGCCATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCGCGCATGGACATCTCGGAAAAGCGTGTCCC
GCAGGACGGGCGCATGAAGCTCAAGGTCGGCCCGGACCGCGTCATCGACTTTCGCGTCAGCACGCTGCCCACGCTTTTCG
GCGAGAAGATCGTGATCCGTATCCTGGACCCGAGCTCGGCCAGGATGGGCATCGACGCCCTGGGCTATGAACCCGAGGAG
AAGGCACGCCTGCTTGAAGCCATCAGCCGCCCCTACGGAATGATTCTGGTCACAGGCCCCACAGGCTCGGGCAAGACAGT
GTCGCTGTACACCTGCCTGAACCTGCTGAACAAGGCCGGCGTGAACATCGCCACGGCGGAAGATCCTTCGGAAATCAACC
TGCCCGGCGTCAACCAGGTCAATGTGAACGAAAAGGCCGGCCTCACCTTTGCCACTGCGCTCAAGGCTTTTCTGCGCCAG
GATCCCGACATCATCATGGTCGGTGAAATCCGCGATCTGGAAACGGCCGACATCTCGATCAAGGCAGCGCAAACCGGCCA
CTTGGTGCTGTCCACCCTGCACACCAATGACGCACCGACCACGTTGACGCGCATGCGCAACATGGGCATTGCGCCCTTCA
ACATCGCATCGAGCGTGATCCTGATCACCGCCCAGCGCCTGGCCAGGCGCCTGTGCCCTCAATGCAAGGAGCCGGCTGAC
ATTCCGCGGGAGGCCCTGATCGATGCAGGCTACGACGATGCGGACATTGACGGCAGCTGGGTCACCTACAGGCCCGTCGG
CTGTCCGGCATGCAACAACGGCTACAAGGGCCGCGTAGGCATCTATCAGGTGATGCCTATCAGCGAGGAAATCCAGCGCA
TCATCCTGCAGGACGGCAGTGCGCTGGAGATCGCCGCACAGGCCAAGCGCGAAGGCGTGCGGTCGCTGCGTGGATCGGGT
TTGCACAAGGCCAGACAGGGACTGACCTCGCTCGAAGAAGTGCTGGCAGTCACCAATGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(57-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.206

97.241

0.547
  pilB Acinetobacter baylyi ADP1

55.712

98.103

0.547
  pilF Neisseria gonorrhoeae MS11

52.12

97.586

0.509
  pilB Legionella pneumophila strain ERS1305867

49.736

98.103

0.488
  pilB Vibrio cholerae strain A1552

46.679

96.034

0.448
  pilB Vibrio campbellii strain DS40M4

44.248

97.414

0.431
  pilB Vibrio parahaemolyticus RIMD 2210633

47.036

90.172

0.424
  pilF Thermus thermophilus HB27

38.889

99.31

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

83.793

0.362

Multiple sequence alignment