Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   A4W86_RS06840 Genome accession   NZ_CP017272
Coordinates   1360099..1361106 (-) Length   335 a.a.
NCBI ID   WP_011375000.1    Uniprot ID   Q38W24
Organism   Latilactobacillus sakei strain TMW 1.1239     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1355099..1366106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W86_RS06800 (A4W86_06750) - 1355434..1355892 (-) 459 WP_035145008.1 hypothetical protein -
  A4W86_RS06805 (A4W86_06755) - 1355947..1357143 (-) 1197 WP_011374993.1 acetate kinase -
  A4W86_RS06810 (A4W86_06760) - 1357165..1358175 (-) 1011 WP_016265372.1 class I SAM-dependent methyltransferase -
  A4W86_RS06815 (A4W86_06765) - 1358299..1358637 (-) 339 WP_035145011.1 hypothetical protein -
  A4W86_RS06820 (A4W86_06770) comGF 1358600..1359112 (-) 513 WP_035145013.1 competence type IV pilus minor pilin ComGF Machinery gene
  A4W86_RS06825 (A4W86_06775) comGE 1359087..1359392 (-) 306 WP_016265375.1 hypothetical protein Machinery gene
  A4W86_RS06830 (A4W86_06780) comGD 1359379..1359831 (-) 453 WP_035145016.1 competence type IV pilus minor pilin ComGD Machinery gene
  A4W86_RS06835 (A4W86_06785) comGC 1359803..1360102 (-) 300 WP_011374999.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A4W86_RS06840 (A4W86_06790) comGB 1360099..1361106 (-) 1008 WP_011375000.1 type II secretion system F family protein Machinery gene
  A4W86_RS06845 (A4W86_06795) comGA 1361099..1361989 (-) 891 WP_016265378.1 competence type IV pilus ATPase ComGA Machinery gene
  A4W86_RS06850 (A4W86_06800) - 1362104..1362835 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  A4W86_RS06855 (A4W86_06805) - 1362926..1363426 (-) 501 WP_099947696.1 VanZ family protein -
  A4W86_RS06860 (A4W86_06810) - 1363523..1363897 (+) 375 WP_011375004.1 hypothetical protein -
  A4W86_RS06870 (A4W86_06820) - 1364084..1364698 (-) 615 WP_251950590.1 hypothetical protein -
  A4W86_RS06875 (A4W86_06825) - 1364695..1365483 (-) 789 WP_251950593.1 hypothetical protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 38450.42 Da        Isoelectric Point: 9.5442

>NTDB_id=197198 A4W86_RS06840 WP_011375000.1 1360099..1361106(-) (comGB) [Latilactobacillus sakei strain TMW 1.1239]
MYKLRQQAQFYADFLQLLGRLLENGFSLQQAFAFLPIVFPKQRQWLNQVNQALEKGDSLAVSLAHADFPTDYVGQIELTE
LQGNLGQCLWHLGRIQSIRQKRRREIRGVLAYPLFLLAFLGGLIGLLRHYLLPEIAQITPESNQTGSPLSRIILWGFLLV
LVLLIMGSGLFMVYRRLPVLVRLQKAFKWPIIGRDIQQYYHYCLLFDLSTCLQNGLKLTEICALQKQLQQNTWLVTLVAQ
LEVVLASGQSLDAYLSRSAFFPQSLRLVLAKHNLTQQTTDEITMLANLQYTELQKQLQRKVSWLQPILFILIGLIIICTY
LSILLPLYQTMEGIS

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=197198 A4W86_RS06840 WP_011375000.1 1360099..1361106(-) (comGB) [Latilactobacillus sakei strain TMW 1.1239]
ATGTATAAGTTACGACAACAAGCGCAATTTTACGCTGATTTCTTGCAGTTATTGGGGCGGCTATTAGAGAACGGTTTCTC
ATTGCAACAAGCTTTTGCCTTCTTACCGATTGTCTTCCCAAAACAACGACAGTGGTTAAATCAGGTGAATCAAGCCCTTG
AAAAAGGCGACAGTCTAGCAGTCAGTTTAGCGCATGCTGATTTTCCAACTGATTATGTTGGTCAGATTGAACTAACCGAA
TTACAAGGTAATCTCGGGCAATGTTTATGGCATTTAGGCCGGATTCAAAGTATCCGCCAAAAACGCCGCCGAGAAATTCG
GGGTGTGCTAGCTTATCCGTTATTTCTTTTGGCTTTTTTAGGCGGCTTAATCGGTTTATTACGCCATTATTTATTACCTG
AGATTGCCCAGATAACGCCCGAGTCAAACCAGACAGGCTCGCCGCTTAGTCGCATCATATTGTGGGGCTTTTTGTTAGTG
CTAGTCCTTTTGATAATGGGCAGCGGTCTTTTTATGGTTTACCGCCGGTTACCGGTTTTAGTTCGATTACAAAAAGCGTT
CAAGTGGCCAATCATTGGGCGTGATATTCAGCAGTATTATCATTATTGTTTATTATTTGATTTGAGCACTTGTCTGCAAA
ACGGGCTTAAGTTAACAGAAATTTGTGCTTTACAGAAGCAACTGCAACAAAATACATGGCTGGTGACCTTGGTAGCACAA
TTGGAAGTCGTACTCGCTTCAGGGCAATCATTGGATGCTTATCTTAGTCGCTCTGCATTTTTTCCACAGTCGTTACGATT
GGTATTAGCTAAGCATAATCTCACGCAACAGACGACCGATGAGATCACCATGCTAGCTAACCTGCAGTATACGGAACTAC
AGAAGCAATTACAGCGGAAAGTGAGCTGGTTACAACCTATTTTATTTATCTTAATTGGGCTCATTATTATTTGTACGTAT
CTCAGTATTTTATTACCGCTGTATCAAACAATGGAGGGAATTTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(207-326)

Type II secretion system (T2SS), protein F (T2SSF)

(14-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q38W24

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Latilactobacillus sakei subsp. sakei 23K

100

100

1

Multiple sequence alignment