Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   A4W86_RS06820 Genome accession   NZ_CP017272
Coordinates   1358600..1359112 (-) Length   170 a.a.
NCBI ID   WP_035145013.1    Uniprot ID   A0A9N7J0G1
Organism   Latilactobacillus sakei strain TMW 1.1239     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1353600..1364112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W86_RS06790 (A4W86_06740) - 1354526..1354891 (+) 366 WP_011374991.1 DUF805 domain-containing protein -
  A4W86_RS06800 (A4W86_06750) - 1355434..1355892 (-) 459 WP_035145008.1 hypothetical protein -
  A4W86_RS06805 (A4W86_06755) - 1355947..1357143 (-) 1197 WP_011374993.1 acetate kinase -
  A4W86_RS06810 (A4W86_06760) - 1357165..1358175 (-) 1011 WP_016265372.1 class I SAM-dependent methyltransferase -
  A4W86_RS06815 (A4W86_06765) - 1358299..1358637 (-) 339 WP_035145011.1 hypothetical protein -
  A4W86_RS06820 (A4W86_06770) comGF 1358600..1359112 (-) 513 WP_035145013.1 competence type IV pilus minor pilin ComGF Machinery gene
  A4W86_RS06825 (A4W86_06775) comGE 1359087..1359392 (-) 306 WP_016265375.1 hypothetical protein Machinery gene
  A4W86_RS06830 (A4W86_06780) comGD 1359379..1359831 (-) 453 WP_035145016.1 competence type IV pilus minor pilin ComGD Machinery gene
  A4W86_RS06835 (A4W86_06785) comGC 1359803..1360102 (-) 300 WP_011374999.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A4W86_RS06840 (A4W86_06790) comGB 1360099..1361106 (-) 1008 WP_011375000.1 type II secretion system F family protein Machinery gene
  A4W86_RS06845 (A4W86_06795) comGA 1361099..1361989 (-) 891 WP_016265378.1 competence type IV pilus ATPase ComGA Machinery gene
  A4W86_RS06850 (A4W86_06800) - 1362104..1362835 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  A4W86_RS06855 (A4W86_06805) - 1362926..1363426 (-) 501 WP_099947696.1 VanZ family protein -
  A4W86_RS06860 (A4W86_06810) - 1363523..1363897 (+) 375 WP_011375004.1 hypothetical protein -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 19689.58 Da        Isoelectric Point: 6.9899

>NTDB_id=197194 A4W86_RS06820 WP_035145013.1 1358600..1359112(-) (comGF) [Latilactobacillus sakei strain TMW 1.1239]
MRLIGRPIKHSAFTLIEMVISMAILILTSQLLVATVGVLQKAIKVTQQQQNTINWHISTIQLDQFLADAYFTHHQDQDGS
KITFRKPDTNQSGEEAETVYYLKRMKNEMVVVSQLNGYMPLLGNLKQLQLDYQRPFVQIDATFTDQSRYQHEVFLEEQDE
QAKKKTIKTD

Nucleotide


Download         Length: 513 bp        

>NTDB_id=197194 A4W86_RS06820 WP_035145013.1 1358600..1359112(-) (comGF) [Latilactobacillus sakei strain TMW 1.1239]
TTGCGATTAATTGGACGACCGATTAAGCATTCAGCGTTCACGCTAATTGAAATGGTGATTTCAATGGCGATTCTAATCCT
GACGAGTCAGTTACTGGTGGCAACGGTGGGTGTTTTACAAAAGGCAATTAAAGTCACGCAACAACAGCAAAATACAATCA
ATTGGCATATCAGTACGATTCAGTTAGATCAGTTTTTAGCCGATGCGTATTTTACACATCATCAGGATCAAGATGGCTCC
AAAATCACTTTTCGAAAACCAGACACTAATCAGTCAGGGGAGGAAGCGGAGACTGTTTACTATTTAAAAAGGATGAAAAA
TGAAATGGTTGTTGTCAGCCAATTAAATGGGTATATGCCATTATTAGGCAACCTCAAGCAACTTCAACTTGACTACCAGC
GACCGTTTGTTCAAATCGATGCGACTTTTACGGATCAGAGTCGCTACCAACACGAAGTTTTCTTGGAGGAACAAGATGAA
CAGGCTAAAAAAAAGACGATTAAAACCGATTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Latilactobacillus sakei subsp. sakei 23K

98.235

100

0.982

Multiple sequence alignment