Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   BHS00_RS01575 Genome accession   NZ_CP017194
Coordinates   320602..321876 (-) Length   424 a.a.
NCBI ID   WP_079507329.1    Uniprot ID   -
Organism   Lactococcus carnosus strain TMW 2.1612     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 315946..330875 320602..321876 within 0


Gene organization within MGE regions


Location: 315946..330875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHS00_RS01540 (BHS00_01525) obgE 316069..317388 (+) 1320 WP_079507341.1 GTPase ObgE -
  BHS00_RS01545 (BHS00_01530) - 317449..317640 (+) 192 WP_079507339.1 hypothetical protein -
  BHS00_RS01550 (BHS00_01535) - 317637..318623 (+) 987 WP_079507337.1 PhoH family protein -
  BHS00_RS01555 (BHS00_01540) - 318623..319076 (+) 454 Protein_285 HIT family protein -
  BHS00_RS01560 (BHS00_01545) ybeY 319079..319570 (+) 492 WP_079507335.1 rRNA maturation RNase YbeY -
  BHS00_RS01565 (BHS00_01550) - 319554..319955 (+) 402 WP_079507333.1 diacylglycerol kinase family protein -
  BHS00_RS01570 (BHS00_01555) - 319958..320500 (-) 543 WP_191245675.1 ComF family protein -
  BHS00_RS01575 (BHS00_01560) comFA/cflA 320602..321876 (-) 1275 WP_079507329.1 DEAD/DEAH box helicase Machinery gene
  BHS00_RS01580 (BHS00_01565) - 321963..322586 (+) 624 WP_079507327.1 YigZ family protein -
  BHS00_RS01590 (BHS00_01575) - 323085..324160 (-) 1076 Protein_291 tyrosine-type recombinase/integrase -
  BHS00_RS01595 (BHS00_01580) - 324270..324806 (-) 537 WP_079507325.1 OB-fold protein -
  BHS00_RS01600 (BHS00_01585) - 324897..325613 (-) 717 WP_079507323.1 helix-turn-helix domain-containing protein -
  BHS00_RS01605 (BHS00_01590) - 325846..326079 (+) 234 WP_418766669.1 helix-turn-helix domain-containing protein -
  BHS00_RS01610 (BHS00_01595) - 326095..326874 (+) 780 WP_079507319.1 DUF3102 domain-containing protein -
  BHS00_RS01615 (BHS00_01600) - 326922..328646 (+) 1725 WP_223265698.1 glycoside hydrolase domain-containing protein -
  BHS00_RS01620 (BHS00_01605) - 328693..329250 (-) 558 WP_079507315.1 hypothetical protein -
  BHS00_RS01625 (BHS00_01610) - 329756..329974 (-) 219 WP_079507313.1 hypothetical protein -
  BHS00_RS01630 (BHS00_01615) - 330001..330207 (+) 207 WP_097025175.1 hypothetical protein -
  BHS00_RS01635 (BHS00_01620) - 330315..330875 (-) 561 WP_079507309.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 424 a.a.        Molecular weight: 47556.04 Da        Isoelectric Point: 9.6691

>NTDB_id=196528 BHS00_RS01575 WP_079507329.1 320602..321876(-) (comFA/cflA) [Lactococcus carnosus strain TMW 2.1612]
MENLFGRLLTQQELGPDMSLLPQDVIRFSGMAIAGTNVICKRCGTASSCQTVRLEIPAYFCPECLQLGRVRSDEFLYHLP
QQPFPRINALLWNGTLTPYQEDISKQLVQAVDQKKQILVHAVTGAGKTEMIYAAISRSIASGGAVCIATPRTDVARELYT
RLSNDFSVPISLLHADSPPYFRTPLVISTTHQLLRFREAFDLLIIDEVDAFPFADNPALYYAAEHAQKTAATLIYLTATS
TETLDKLVSSDLLKRITLSRRFHGHPLVVPKPIFSYPEKIIYRHIQKQRQTGFPLLLFAPVIRFGQSFTAQLSKLFPNER
IGFVASTTENRSEIISQFRKGELTILVSTTILERGVTFPKVDVFVLESQHRLFTASSLIQIAGRAGRSIERPTGLVYFFH
NGLTKQMTRAISDIRKMNRLGGFS

Nucleotide


Download         Length: 1275 bp        

>NTDB_id=196528 BHS00_RS01575 WP_079507329.1 320602..321876(-) (comFA/cflA) [Lactococcus carnosus strain TMW 2.1612]
ATGGAAAACTTATTTGGCCGACTACTCACCCAGCAAGAACTGGGCCCTGACATGTCCCTTTTACCACAGGATGTCATCCG
GTTTTCTGGTATGGCAATAGCAGGAACGAATGTCATCTGTAAGCGGTGTGGTACTGCATCCTCTTGCCAAACAGTCCGAC
TTGAAATCCCGGCCTATTTTTGTCCTGAATGTCTGCAACTAGGTCGTGTTAGATCTGATGAATTTCTCTATCACTTACCG
CAACAGCCTTTCCCACGGATAAATGCGTTACTTTGGAATGGCACACTGACGCCTTATCAGGAAGACATCTCTAAGCAACT
CGTACAGGCAGTGGATCAAAAAAAGCAAATTCTAGTTCATGCGGTAACAGGTGCTGGTAAAACAGAGATGATTTATGCGG
CGATTAGCAGAAGTATAGCTAGCGGTGGTGCTGTTTGTATCGCAACACCTAGAACTGACGTCGCTCGCGAGCTATATACG
CGTTTATCTAACGATTTTTCTGTCCCTATCTCGCTCCTGCATGCGGATAGTCCCCCTTACTTTCGTACACCACTTGTTAT
TTCGACAACGCATCAGCTTCTCCGATTTAGAGAGGCATTTGATTTGCTGATCATAGACGAAGTAGATGCCTTTCCTTTCG
CTGATAACCCTGCACTTTACTATGCAGCTGAGCATGCACAAAAAACAGCGGCTACTCTGATTTATCTGACAGCCACTTCT
ACTGAAACCCTAGACAAACTCGTCAGCTCAGACCTATTAAAACGGATTACCTTATCCCGTCGCTTTCACGGTCACCCACT
CGTTGTTCCCAAACCAATTTTTAGCTATCCGGAAAAAATCATCTACCGGCATATCCAAAAACAGCGCCAAACTGGCTTCC
CCTTATTACTATTTGCACCTGTCATTCGGTTTGGGCAATCATTTACGGCCCAGTTAAGCAAGCTTTTTCCCAACGAGAGG
ATAGGGTTTGTTGCATCCACTACTGAAAATCGCTCTGAGATCATCTCCCAGTTTCGCAAAGGTGAGTTAACGATTCTAGT
TTCTACCACCATATTAGAACGCGGGGTCACGTTTCCGAAAGTTGATGTCTTTGTACTAGAAAGTCAGCATCGACTCTTTA
CAGCCTCTAGTCTAATACAGATTGCTGGACGGGCTGGACGAAGTATTGAGCGGCCGACTGGTCTTGTCTATTTTTTTCAT
AATGGCCTAACAAAGCAGATGACCCGTGCTATTTCTGATATTCGCAAGATGAATCGACTTGGTGGGTTCTCATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(95-239)

Helicase conserved C-terminal domain (Helicase_C)

(281-387)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis SK321

53.596

100

0.545
  comFA/cflA Streptococcus pneumoniae Rx1

53.364

100

0.542
  comFA/cflA Streptococcus pneumoniae D39

53.364

100

0.542
  comFA/cflA Streptococcus pneumoniae R6

53.364

100

0.542
  comFA/cflA Streptococcus pneumoniae TIGR4

53.132

100

0.54
  comFA/cflA Streptococcus mitis NCTC 12261

51.972

100

0.528
  comFA Lactococcus lactis subsp. cremoris KW2

55

94.34

0.519

Multiple sequence alignment