Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BS16045_RS13300 Genome accession   NZ_CP017112
Coordinates   2531367..2532437 (-) Length   356 a.a.
NCBI ID   WP_069322755.1    Uniprot ID   -
Organism   Bacillus subtilis strain BS16045     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2526367..2537437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS16045_RS13255 (BS16045_02612) tapA 2526846..2527607 (-) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  BS16045_RS13260 (BS16045_02613) yqzG 2527879..2528205 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  BS16045_RS13265 (BS16045_02614) spoIITA 2528247..2528426 (-) 180 WP_003230176.1 YqzE family protein -
  BS16045_RS13270 (BS16045_02615) comGG 2528497..2528871 (-) 375 WP_029726722.1 ComG operon protein ComGG Machinery gene
  BS16045_RS13275 (BS16045_02616) comGF 2528872..2529255 (-) 384 WP_029317913.1 ComG operon protein ComGF Machinery gene
  BS16045_RS13280 (BS16045_02617) comGE 2529281..2529628 (-) 348 WP_069322754.1 ComG operon protein 5 Machinery gene
  BS16045_RS13285 (BS16045_02618) comGD 2529612..2530043 (-) 432 WP_014480256.1 comG operon protein ComGD Machinery gene
  BS16045_RS13290 (BS16045_02619) comGC 2530033..2530329 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  BS16045_RS13295 (BS16045_02620) comGB 2530343..2531380 (-) 1038 WP_029317912.1 comG operon protein ComGB Machinery gene
  BS16045_RS13300 (BS16045_02621) comGA 2531367..2532437 (-) 1071 WP_069322755.1 competence protein ComGA Machinery gene
  BS16045_RS22925 (BS16045_02622) - 2532649..2532846 (-) 198 WP_069322756.1 CBS domain-containing protein -
  BS16045_RS13315 (BS16045_02623) corA 2532848..2533801 (-) 954 WP_015483432.1 magnesium transporter CorA -
  BS16045_RS13320 (BS16045_02624) yqhB 2533944..2535272 (+) 1329 WP_003230150.1 hemolysin family protein -
  BS16045_RS13325 (BS16045_02625) rsbRD 2535325..2536161 (-) 837 WP_015714260.1 RsbT co-antagonist protein RsbRD -
  BS16045_RS13335 (BS16045_02627) mgsR 2536385..2536765 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  BS16045_RS13340 (BS16045_02628) yqgY 2536997..2537242 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40457.66 Da        Isoelectric Point: 9.1812

>NTDB_id=195707 BS16045_RS13300 WP_069322755.1 2531367..2532437(-) (comGA) [Bacillus subtilis strain BS16045]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIQQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=195707 BS16045_RS13300 WP_069322755.1 2531367..2532437(-) (comGA) [Bacillus subtilis strain BS16045]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAAGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCCAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGTCGCCAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGGAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997
  pilB Glaesserella parasuis strain SC1401

43.878

82.584

0.362

Multiple sequence alignment