Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BARD7_RS11005 Genome accession   NZ_CP016913
Coordinates   2256665..2257735 (-) Length   356 a.a.
NCBI ID   WP_065981884.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain RD7-7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2251665..2262735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BARD7_RS10955 (BARD7_02248) sipW 2251724..2252308 (-) 585 WP_065981877.1 signal peptidase I SipW -
  BARD7_RS10960 (BARD7_02249) tapA 2252280..2252951 (-) 672 WP_065981878.1 amyloid fiber anchoring/assembly protein TapA -
  BARD7_RS10965 (BARD7_02250) - 2253209..2253538 (+) 330 WP_045510605.1 DUF3889 domain-containing protein -
  BARD7_RS10970 (BARD7_02251) - 2253579..2253758 (-) 180 WP_016938971.1 YqzE family protein -
  BARD7_RS10975 (BARD7_02252) comGG 2253815..2254192 (-) 378 WP_065981879.1 competence type IV pilus minor pilin ComGG Machinery gene
  BARD7_RS10980 comGF 2254194..2254694 (-) 501 WP_231131085.1 competence type IV pilus minor pilin ComGF -
  BARD7_RS10985 (BARD7_02254) comGE 2254603..2254917 (-) 315 WP_065981881.1 competence type IV pilus minor pilin ComGE Machinery gene
  BARD7_RS10990 (BARD7_02255) comGD 2254901..2255338 (-) 438 WP_065981882.1 competence type IV pilus minor pilin ComGD Machinery gene
  BARD7_RS10995 (BARD7_02256) comGC 2255328..2255636 (-) 309 WP_081309506.1 competence type IV pilus major pilin ComGC Machinery gene
  BARD7_RS11000 (BARD7_02257) comGB 2255641..2256678 (-) 1038 WP_065981883.1 competence type IV pilus assembly protein ComGB Machinery gene
  BARD7_RS11005 (BARD7_02258) comGA 2256665..2257735 (-) 1071 WP_065981884.1 competence type IV pilus ATPase ComGA Machinery gene
  BARD7_RS11010 (BARD7_02259) - 2257928..2258878 (-) 951 WP_065981885.1 magnesium transporter CorA family protein -
  BARD7_RS11015 (BARD7_02260) - 2259025..2260326 (+) 1302 WP_065981886.1 hemolysin family protein -
  BARD7_RS11020 (BARD7_02261) - 2260364..2261194 (-) 831 WP_065981887.1 STAS domain-containing protein -
  BARD7_RS11030 (BARD7_02263) - 2261396..2261776 (-) 381 WP_013352876.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BARD7_RS11035 (BARD7_02264) - 2262012..2262257 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40483.82 Da        Isoelectric Point: 9.5925

>NTDB_id=193635 BARD7_RS11005 WP_065981884.1 2256665..2257735(-) (comGA) [Bacillus amyloliquefaciens strain RD7-7]
MKNIETYSRNIINEAYMARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLNKLSLFPGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSSGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKTDNCSVYCRLYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=193635 BARD7_RS11005 WP_065981884.1 2256665..2257735(-) (comGA) [Bacillus amyloliquefaciens strain RD7-7]
ATGAAGAATATTGAAACATACAGCAGGAACATCATCAACGAGGCCTATATGGCAAGGGCTTCAGATATACACATTGTGCC
TAGGGAAAGGGATGCTTTCATTCATTTTCGCATCGGTCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAATTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGATCACTGTCT
CTGCCGCTTCCAACGGAAACCGTTCATTTGAGGATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
ATTGCCTAAGAGGCAGATTCCCCCTCTGAATAAACTGTCCTTATTTCCGGGGGCAGGCGCCGCATTATTATCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTTTTTACCGGACCGACCGGATCTGGAAAAACAACCACCCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTGGAAGATCCCGTAGAAACGAGAGATGAAGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCTCCGGTTTAAAAGCGATTTTAAGACATGACCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACAGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTACTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGGGTGAACATGACGGAAATTGAACAAACCGTTATTGC
CATCGCCGCTCAGCGTCTGGTTGATTTAACGTGTCCGTTTTGCAAAACGGACAACTGTTCCGTGTACTGCCGTCTGTACA
GGCAGACACGCCGTGCGGGGATTTATGAACTGCTTTACGGAAAAAATCTCACCCAGTGCTTTCAGGAAGCAAAAGGAGAG
CACGCCAATTTTCAATATCAGACGCTTCGCAGGCTGATCAGAAAAGGTATCGCGCTCGGTTATGTCACGACTGACAATTA
TGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.04

97.472

0.371

Multiple sequence alignment