Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   BARD7_RS05095 Genome accession   NZ_CP016913
Coordinates   1042605..1046309 (+) Length   1234 a.a.
NCBI ID   WP_065982860.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain RD7-7     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1037605..1051309
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BARD7_RS05080 (BARD7_01031) - 1037667..1038194 (-) 528 WP_065981121.1 ferritin-like domain-containing protein -
  BARD7_RS05085 (BARD7_01032) - 1038263..1038976 (-) 714 WP_081309495.1 DUF421 domain-containing protein -
  BARD7_RS05090 (BARD7_01033) addB 1039118..1042618 (+) 3501 WP_065981122.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BARD7_RS05095 (BARD7_01034) addA 1042605..1046309 (+) 3705 WP_065982860.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BARD7_RS05100 (BARD7_01035) sbcD 1046376..1047548 (+) 1173 WP_065981123.1 exonuclease subunit SbcD -
  BARD7_RS05105 (BARD7_01036) sbcC 1047545..1050937 (+) 3393 WP_065981124.1 exonuclease subunit SbcC -
  BARD7_RS05110 (BARD7_01037) - 1050953..1051252 (+) 300 WP_065981125.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141263.86 Da        Isoelectric Point: 5.2558

>NTDB_id=193608 BARD7_RS05095 WP_065982860.1 1042605..1046309(+) (addA) [Bacillus amyloliquefaciens strain RD7-7]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEEPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELVDRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLKSFVRLYDVTEESKMEELPFYEYVREDAEMALFGAKQKLEKA
LELTKAPGGPAPRADNILDDLQQIEELISCRHDFKALYERVPTVTFKRAKAVKGDEFDKALLDEATDLRNGAKKLIEKLK
TDYFTRSPQDHLKSLKDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTARDEEGRRVPSEAAVYYQDQ
FHEVLVDEYQDTNLVQESILQLVKSGNEESGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTENGEGAGQKIDLNQNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAAKTELLLIDSADDADSPEDAEDFETVHWEAKAIAAEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANGDRHDDLYQKLRAFYDSLQKWRSFSTNHSVSELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQAQADRADWLLSEFDRYQASSYLDFIGPALIRHRDMEAHRAPGLLAPEDIAHDPARFHIRMLQQSE
LLEDSPEERAEEKSKRLKAIQHGEPIPDSFSFDDQARRLLEWEYPYRKLTAIRTKQSVSELKRKQEYEDEYSGRSLIKPS
GDTLLYKRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHAPTAEEAERTVRMLYEKELLTEEQLDAIDIDELIQFFGTEIGK
DLVGALRIDREVPFSMALPASEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAVEQITKTKVKGRALYFFDGGHVLTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=193608 BARD7_RS05095 WP_065982860.1 1042605..1046309(+) (addA) [Bacillus amyloliquefaciens strain RD7-7]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTTGTAGAGCGGATGATCAGAAAGATAACCGCGGAAGAAGAGC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACATTTACAAACGCCTCAGCAGCGGAAATGAAGCATCGGATTGCTGAAGCA
TTAGAAAAAGAACTCGCCAAAAATCCGGGGTCTCTGCATATCAGACGGCAGCTGTCTCTATTAAATCGGGCGAGCATTTC
GACGCTTCACTCTTTCTGTCTGCAGGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCGGGTTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTGCTTGATGAGCTGTTTGAGGATGAATACGCAAAAGGGAATCAGGCG
TTTTTTGAGCTTGTGGACAGATATACGACGGACAGGCACGATCTGGATCTTCAAGACCTCGTCAAGCGGGTGTATGAGTA
TTCCCGGTCGCATCCTGACCCGGAAGCGTGGCTGAAAAGCTTTGTCCGCCTATATGATGTGACAGAAGAAAGCAAAATGG
AAGAGCTGCCGTTTTACGAGTATGTGAGAGAAGATGCTGAAATGGCGCTCTTCGGGGCAAAACAAAAGCTAGAGAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCCCCGCGTGCTGATAATATTCTGGATGACCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTTAAGGCGCTGTATGAGCGGGTTCCTACGGTCACATTTAAACGGGCCAAGGCGGTAAAGG
GTGATGAATTTGACAAGGCGCTTTTGGACGAAGCGACTGATTTGCGAAACGGCGCGAAGAAACTGATCGAAAAGTTAAAA
ACCGATTACTTTACAAGAAGCCCGCAAGATCATTTAAAGAGTTTAAAAGACATGAAGCCTGTCATTGAAACCCTCGTTCA
GCTCGTAATATCGTACGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATCATTGATTTCTCAGATTTGGAACATT
ACTGCCTGGCGATTTTAACTGCGAGGGATGAAGAAGGCCGGCGTGTGCCGAGTGAAGCGGCCGTTTATTATCAAGACCAG
TTCCATGAAGTTCTGGTTGATGAATATCAAGATACCAATCTTGTGCAGGAATCCATTTTGCAGCTTGTTAAAAGCGGGAA
TGAAGAGTCGGGAAATCTGTTTATGGTGGGGGACGTCAAGCAATCAATCTACCGTTTCAGACTTGCCGAGCCGCTGCTTT
TTCTCGGGAAATACAAAAGGTTTACGGAAAATGGGGAAGGCGCCGGGCAGAAAATCGACTTGAACCAAAACTTCCGAAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGCAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTAAAGCTTGGCGCATCGTATCCGCCGAATGACGCGGCGAAAACGGAACTGCTGCTGATTGACAGCGCTG
ATGACGCAGACAGCCCCGAGGATGCCGAAGATTTTGAAACCGTGCATTGGGAAGCGAAAGCGATAGCCGCGGAGATCCGG
AAGCTTGTCTCATCTCCGTTTAAGGTATATGACGGCAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTGAT
TTTGCTGCGTTCCATGCCATGGGCTCCGCAATTAATGGAAGAGCTGAAAAATCAGGGCATCCCGGTTTACGCCAATTTGA
CGTCAGGTTACTTTGAAGCCGTGGAGGTGGCTGCGGCGCTTTCAGTCTTAAAGGTGATTGATAATCCGTATCAGGATATT
CCGCTAGCTTCCGTGCTGCGCTCGCCCATCGTCGGCTGTGACGAAAATGAACTCGCGCTCATACGTCTTGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCGTATATAGCGAACGGTGACAGACATGACGACCTGTATCAAAAACTGAGAGCAT
TTTATGACAGTCTGCAGAAGTGGCGGTCATTCTCGACGAATCATTCAGTGTCTGAGCTGATTTGGGAAGTATACCGCGAT
ACCGGATATTTTGATTATGCAGGCGGCATGCCTGGCGGAAAGCAGCGGCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGTTCGTATGAAGCGACGGCATTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATTTGGGGACGGCTCGGGCGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAA
TTCCCCGTCGTTTTCACCGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGA
GCTCGGTTTCGGCACGAAGTATATTCATCCGGAACTTAGAATCAGCTATCCGACTCTGCCGCTTGTGGCAATGAAGAAAA
AAATGCGCAGAGAGCTTTTATCAGAAGAATTGCGCGTATTGTATGTGGCGCTGACAAGGGCGAAGGAAAAGCTGTTTCTC
GTCGGTTCCTGCAAAAACCGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCAGACCGGGCCGATTGGCTCCTTTCTGA
ATTCGACCGCTACCAGGCGTCATCTTATTTGGACTTTATCGGTCCGGCGCTCATCCGTCACCGTGACATGGAGGCGCACA
GGGCTCCGGGGCTTTTGGCGCCAGAAGATATCGCGCACGACCCGGCGCGTTTTCACATCCGGATGCTGCAGCAAAGTGAA
TTGCTGGAAGATAGTCCTGAGGAACGTGCTGAAGAAAAGAGCAAACGTTTAAAAGCGATACAGCATGGCGAGCCGATTCC
TGATTCGTTTTCATTTGATGATCAGGCGCGCCGTCTTCTTGAATGGGAATATCCGTATCGGAAGCTGACCGCCATCAGAA
CGAAGCAGTCTGTTTCTGAGCTGAAGAGAAAGCAAGAATACGAGGATGAGTACAGCGGACGCTCTCTCATCAAACCGTCA
GGGGACACTCTTTTATACAAGCGTCCCGGCTTTATGATGAAAAAAGGGCTGACAGCCGCCGAAAAAGGGACGGCGATGCA
TACGGTGATGCAGCACATACCGCTGACGCATGCTCCGACTGCTGAAGAAGCGGAGCGGACGGTCCGGATGCTTTACGAAA
AAGAGCTGCTGACAGAAGAACAGCTGGACGCAATTGATATCGATGAGCTGATTCAGTTTTTCGGAACTGAAATCGGAAAA
GACCTTGTTGGAGCGCTGCGGATTGATCGGGAAGTGCCGTTCAGTATGGCGCTTCCGGCCAGCGAGGTGTACAAGGATGC
CGAAACGGCCGGTGAACCGCTGCTCGTCCAGGGAATTATTGACTGCCTCTACGAAACTGCGGACGGACTCTATTTGCTGG
ACTATAAAACGGATCGGATCGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCCCCGATCCTGCAAAAAAGGTATGAA
ACACAAATCGAATTGTATACAAAAGCGGTTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTCTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

82.091

100

0.821

Multiple sequence alignment