Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   BARD7_RS05090 Genome accession   NZ_CP016913
Coordinates   1039118..1042618 (+) Length   1166 a.a.
NCBI ID   WP_065981122.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain RD7-7     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1034118..1047618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BARD7_RS05065 (BARD7_01028) - 1034285..1035442 (-) 1158 WP_065981119.1 AbrB family transcriptional regulator -
  BARD7_RS05070 (BARD7_01029) - 1035608..1036804 (-) 1197 WP_065981120.1 MFS transporter -
  BARD7_RS05075 (BARD7_01030) - 1036820..1037440 (-) 621 WP_065521730.1 TetR/AcrR family transcriptional regulator -
  BARD7_RS05080 (BARD7_01031) - 1037667..1038194 (-) 528 WP_065981121.1 ferritin-like domain-containing protein -
  BARD7_RS05085 (BARD7_01032) - 1038263..1038976 (-) 714 WP_081309495.1 DUF421 domain-containing protein -
  BARD7_RS05090 (BARD7_01033) addB 1039118..1042618 (+) 3501 WP_065981122.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BARD7_RS05095 (BARD7_01034) addA 1042605..1046309 (+) 3705 WP_065982860.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BARD7_RS05100 (BARD7_01035) sbcD 1046376..1047548 (+) 1173 WP_065981123.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 133728.37 Da        Isoelectric Point: 5.2384

>NTDB_id=193607 BARD7_RS05090 WP_065981122.1 1039118..1042618(+) (addB) [Bacillus amyloliquefaciens strain RD7-7]
MGVEFLVGRSGSGKTRLIIDSIQEELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEHKHDFKVYQKASDKTGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQIPLADVIKGARVYVDGFYQFTPQELRVLEQLIMHAEHVTFSLTADSP
SAEQAPDELDLFRMTGSTYYKLYQMAKELNADISCKELHGTKRHQQVPELACIESQYDVRPAAVYTGGQEAFTVIQAQNR
RAELEGIAREIQSLVRDRGYRYRDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPEQKIREQVDQLENYCIAYGIKGERWTNGERFVYRRFVSLDEDFAQTDQEIEMEHMLNETREW
MAEPLIRLQNRLKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQQAEEAGNMIEAQQHGQAWDAVIQLLEEFAGMMGEDEM
SLALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLKAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVTYPIADAEGKTLLPSIVVNRLGELFPDHQEKLSAADPEQVSEEEQLQYLVNKQ
VTQTYTASQLRLWTREYEISDVWWSAYNVLMKEPDHERAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNA
CQFSHFASHGLQLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYDAVERLAPRLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGSGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMKATPAGVLYFHIHDPMIQASLPMELDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIINAGLKKDGSLRSDSAAVSERDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFKSVCQFDESLKENEYRSLKAEKDGTILDWLKKEADDDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=193607 BARD7_RS05090 WP_065981122.1 1039118..1042618(+) (addB) [Bacillus amyloliquefaciens strain RD7-7]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGAAGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGCTTTTCGCGGTTGGCGTGGCGCGTGCTTCAGCACACGGGG
GGCATGAACCGTCCGTTTGTGACGACGACGGGTGTGCAGATGCTTTTAAGGAAACTGATTGAAGAACATAAGCACGATTT
TAAAGTGTATCAAAAAGCGAGTGACAAAACTGGATTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGGTACT
GCATTGAGCCTGAAGATGTCCGCCGCATGGCGGAAAGCGGAACGGCGTCAGAATACCGCGGAGAGCGGATGCTGTCTGAA
AAACTTCATGACTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGCCACTATTTGCATTCAGAGGATTATTT
GACGCTGCTCGCTCAGCAGATTCCTTTGGCGGATGTTATAAAAGGCGCCCGTGTTTATGTCGACGGCTTCTATCAATTTA
CGCCGCAGGAATTAAGGGTGCTCGAGCAGCTGATCATGCATGCTGAACATGTCACATTCTCTCTGACGGCGGACAGTCCT
TCGGCGGAACAGGCTCCGGATGAGCTCGATCTGTTCAGAATGACCGGCAGCACATATTATAAGCTTTATCAGATGGCAAA
GGAGTTGAATGCCGATATTTCCTGCAAGGAATTGCATGGAACGAAGCGGCATCAGCAAGTGCCGGAGCTTGCCTGTATTG
AATCGCAGTACGATGTGCGTCCCGCGGCTGTGTATACTGGCGGACAGGAAGCTTTTACCGTCATACAGGCGCAGAACAGA
AGAGCTGAACTCGAGGGTATTGCCCGGGAAATTCAATCACTTGTCCGGGACCGAGGATACCGGTACAGAGATATGGCGAT
TCTCATCCGTCAGCCGGAAGATTATAAAGATTTGTTGAAGGAAGTGTTTGCAGATTACGGGCTTCCTTATTTTATAGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATTGAATTCATCCGCTCAAGCTTGGATGTCGTGAAGGGGAACTGGAGATAT
GAAGCGGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCCCTTGATCAGCCGGAGCAGAAAATAAGAGAGCAGGTTGA
TCAGCTTGAAAACTACTGTATCGCCTACGGAATCAAAGGTGAGCGCTGGACGAACGGGGAGCGGTTTGTATACAGGCGGT
TCGTATCGCTCGATGAGGATTTCGCGCAGACGGATCAGGAAATAGAGATGGAACACATGCTGAATGAGACGAGAGAGTGG
ATGGCCGAGCCGCTCATCCGGCTTCAAAACCGGCTGAAAAAAGCGAAAACGGTTCAAAGCATGGCGGAAGCTCTTTATCT
CTTTTTGGAAGATACCGATGTTCCCCTGAAGCTGGATCGGAAAAGACAGCAGGCCGAAGAGGCCGGGAACATGATTGAAG
CGCAGCAGCACGGCCAGGCGTGGGATGCCGTTATCCAGCTTCTCGAAGAGTTTGCGGGAATGATGGGTGAAGATGAAATG
TCTTTGGCGCTGTTCCAGCAAATGCTTGAAACGGGAACGGAATCGCTTCATTTTTCTTTAATTCCTCCTGCGCTTGACCA
AGTATTTGTAGGGAATATGGATCTGTCCAGAATGTACGGCACCTCCTGCACGTTTGTCATCGGGGCCAATGACGGCGTTC
TTCCGGCGCGCCCGGATGAAAACGGCGTGCTGTCTGATGATGACCGGGAGTGGCTGAAAGCGGTCGGAGTGGAGCTTTCT
TCCGCGGGGCGTGAGCGGCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCAAGTCCGTCTGACCGTCTGTATGT
GACATATCCGATTGCCGATGCGGAAGGAAAAACGCTCCTGCCGTCAATCGTTGTAAACAGGCTCGGCGAGCTGTTTCCCG
ATCATCAGGAAAAGCTGTCAGCGGCTGATCCCGAGCAGGTAAGCGAAGAAGAACAGCTTCAGTATCTCGTCAATAAACAG
GTGACGCAGACGTATACGGCAAGCCAGCTCAGGCTGTGGACAAGAGAGTACGAGATCAGTGACGTGTGGTGGAGCGCATA
CAATGTGCTGATGAAAGAGCCTGATCACGAGCGTGCGAAAAAGCTGTTTTCAAGCCTGTTTTTCCGAAATGAGGCAAAGC
GTCTTGAGCGGCCTGTTTCGCGCCAGCTTTACGGCGAGCACATTAAAGGAAGCGTCTCCCGGATGGAGGCGTTTAATGCC
TGCCAGTTTTCTCATTTTGCATCTCACGGGCTGCAATTAAAAGAGCGGCAGTTTTTTAAGCTGGAAGCGCCGGATATCGG
ACAGCTGTTTCACTCCAGCCTGAAGCTGATTTCAGACCGGCTTCGTGAGCAGAAGCTGGAATGGCGCGATCTGACGAAAG
ATCAGTGCCGGAATTTTTCCTATGATGCGGTGGAACGGCTTGCGCCAAGGCTGCAAAAAGAGATTCTGCTCAGCTCAAAC
AGGCACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTCTCAGGCATATTAAGCGAGCATGCGAAGGCGAG
CGGTTTCGTCCCGATCGGCCTTGAACTCGGTTTCGGCGGAAGCGGACCGCTGCCTCCGCTCACTTTCACGCTGAAAAACG
GCTGTACGATGGAACTCGTCGGCCGGATCGACCGCGTGGATAAAGCGGAAAGCTCAAAAGGCCTGCTGCTGAGAATCGTT
GATTATAAATCAAGCGACAAGGGGCTTGATCTCGCTGAGGTATATTACGGGCTCGCTCTGCAAATGCTCACATACCTTGA
CTTATCAATTACACACTCAGCGGATTGGCTCGGCATGAAAGCGACACCGGCAGGCGTTTTGTATTTCCATATTCATGATC
CGATGATTCAGGCCAGTCTGCCGATGGAACTTGATGAAATCGAGCAGGAAATCTTTAAGAAATTTAAAATGAAAGGCCTT
CTCCTCGGTGACCGGGAGGCGATCAGCCTGATGGACACGACTTTGGAGGAAGGGCGTTCAAATATTATCAATGCCGGTTT
GAAAAAGGACGGTTCTTTACGGTCCGATTCAGCGGCGGTCAGCGAACGGGATTTTCATCTCTTAACAGACCATGTGAGGC
GGACATTTGAGCAGGCGGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCGTTTAAATCGGTGTGCCAGTTTGATGAGTCGCTTAAGGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACTATACTGGATTGGCTGAAAAAGGAGGCGGATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(307-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

83.105

100

0.831

Multiple sequence alignment