Detailed information NTG0193269

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BE846_RS14000
• Genome accession: NZ_CP016876
• Coordinates: 3108705..3109568 (-)
• Length: 287 a.a.
• NCBI ID: WP_011215548.1
• Uniprot ID: Q5WWF1
• Organism: Legionella pneumophila subsp. pneumophila strain FFI337
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3103705..3114568

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BE846_RS13975 (BE846_13975)	-	3103797..3105245 (+)	1449	WP_011215544.1	bifunctional 2-methylcitrate dehydratase/aconitate hydratase	-
BE846_RS13980 (BE846_13980)	-	3105365..3106180 (-)	816	WP_011215545.1	pyruvate, water dikinase regulatory protein	-
BE846_RS13985 (BE846_13985)	-	3106314..3106769 (+)	456	WP_011215546.1	hypothetical protein	-
BE846_RS13990 (BE846_13990)	-	3106785..3107378 (+)	594	WP_011213808.1	hypothetical protein	-
BE846_RS13995 (BE846_13995)	-	3107486..3108691 (+)	1206	WP_011215547.1	cyclic nucleotide-binding domain-containing protein	-
BE846_RS14000 (BE846_14000)	pilD	3108705..3109568 (-)	864	WP_011215548.1	prepilin peptidase	Machinery gene
BE846_RS14005 (BE846_14005)	pilC	3109614..3110834 (-)	1221	WP_011215549.1	type II secretion system F family protein	Machinery gene
BE846_RS14010 (BE846_14010)	pilB	3110840..3112564 (-)	1725	WP_011215550.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BE846_RS14015 (BE846_14015)	-	3112823..3113566 (+)	744	WP_011215551.1	methyltransferase domain-containing protein	-
BE846_RS14020 (BE846_14020)	-	3113749..3114075 (+)	327	WP_011213802.1	DUF1820 family protein	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 32759.36 Da
• Isoelectric Point: 8.0781

>NTDB_id=193269 BE846_RS14000 WP_011215548.1 3108705..3109568(-) (pilD) [Legionella pneumophila subsp. pneumophila strain FFI337]
MINALIINYPWFMYLLVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHFEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCGSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNVFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM

Nucleotide

• Length: 864 bp

>NTDB_id=193269 BE846_RS14000 WP_011215548.1 3108705..3109568(-) (pilD) [Legionella pneumophila subsp. pneumophila strain FFI337]
ATGATAAACGCACTAATAATCAATTATCCATGGTTTATGTATTTACTCGTTGGCCTCTTTTCTCTAGCCGTTGGAAGTTT
GCTTAATGTGATAATCTATCGCTTACCAATTATTTTGCAGGAAGAGTGGAAAGAGCAATGCTGTGAGCTATTCCATTTTG
AACAGCGAAAAGAAAAAATAAAACTTAATTTATTTTTACCACGTTCTTTTTGTCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCTCTGTTAAGCTATATTTTGTTAAGAGGACGCTGTTACCAATGTGGTAGTCCAATTTCAATCCGCTA
CCCTTTTGTTGAGACGTTGACTACAGTTTTATCCTTATACGCCAGTTGGCACTTTGGGTTTACAATACAATTATTATTTG
CTTTATTAGCTATCTGGATATTAATATCACTTGTTTTTATTGATCTTGATCATCAATTACTCCCTGATAGCCTCACTTTA
GGGCTCTTATGGATTGGCCTTATTGCTAATACCCAGAATGTATTTGTTAGTCTTGACGTTGCTGTATTAAGCTGCGCTGG
CGCCTATTTAGCCTTATGGTTATTCATTAATCTGTTTTATCTGATGACAGGTAAAGTAGGTATGGGACATGGAGATTTCA
AATTATTTGCAGCATTTGGTGCCTGGCTTGGATGGATGTATTTACCATTAATTCTGCTAATATCTTCAATAACGGGGGCT
ATCATTGGTTTGATTTATTTAAAGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGTCCTTTTCTTTGTATTTC
TGGATTAATAGCTATGTTTTGGGGAGACTCAATTATCAATTGGTATATAGGTTATTGGATGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

3D structure

• AlphaFold DB: Q5WWF1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.466	97.909	0.484
pilD	Acinetobacter nosocomialis M2	47.985	95.122	0.456
pilD	Acinetobacter baumannii D1279779	47.273	95.819	0.453
pilD	Vibrio campbellii strain DS40M4	45.29	96.167	0.436
pilD	Neisseria gonorrhoeae MS11	42.806	96.864	0.415