Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilX   Type   Machinery gene
Locus tag   A6L31_RS01445 Genome accession   NZ_CP016683
Coordinates   249421..249894 (-) Length   157 a.a.
NCBI ID   WP_002248276.1    Uniprot ID   -
Organism   Neisseria meningitidis strain M22191     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 202659..260086 249421..249894 within 0


Gene organization within MGE regions


Location: 202659..260086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6L31_RS01160 (A6L31_01160) - 202659..204884 (+) 2226 WP_002220751.1 NADP-dependent isocitrate dehydrogenase -
  A6L31_RS13135 - 206052..206186 (+) 135 Protein_220 IS110 family transposase -
  A6L31_RS01170 (A6L31_01170) - 206250..207257 (+) 1008 Protein_221 IS5 family transposase -
  A6L31_RS01175 (A6L31_01175) - 207395..208363 (+) 969 Protein_222 IS5 family transposase -
  A6L31_RS14340 (A6L31_01180) - 208767..208898 (+) 132 WP_002217434.1 hypothetical protein -
  A6L31_RS01185 (A6L31_01185) - 208895..209263 (+) 369 WP_002220735.1 type II toxin-antitoxin system death-on-curing family toxin -
  A6L31_RS13795 - 209279..209449 (+) 171 WP_164728559.1 hypothetical protein -
  A6L31_RS01190 (A6L31_01190) - 209701..210192 (+) 492 WP_002217437.1 DUF4760 domain-containing protein -
  A6L31_RS01195 (A6L31_01195) - 210551..210787 (+) 237 WP_002217439.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  A6L31_RS01200 (A6L31_01200) - 210789..211136 (+) 348 WP_002217440.1 type II toxin-antitoxin system PemK/MazF family toxin -
  A6L31_RS01205 (A6L31_01205) - 211189..211698 (-) 510 WP_002220734.1 hypothetical protein -
  A6L31_RS01210 (A6L31_01210) - 211710..212183 (-) 474 WP_002220733.1 D-Ala-D-Ala carboxypeptidase family metallohydrolase -
  A6L31_RS01215 (A6L31_01215) - 212322..212597 (-) 276 WP_002217443.1 hypothetical protein -
  A6L31_RS01220 (A6L31_01220) - 212594..213019 (-) 426 WP_002220721.1 hypothetical protein -
  A6L31_RS01225 (A6L31_01225) - 213083..213454 (-) 372 WP_002220720.1 hypothetical protein -
  A6L31_RS14435 - 213522..213728 (-) 207 WP_002217448.1 hypothetical protein -
  A6L31_RS01230 (A6L31_01230) - 213745..214191 (-) 447 WP_002220718.1 hypothetical protein -
  A6L31_RS01235 (A6L31_01235) gpJ 214262..218527 (-) 4266 WP_065826016.1 TipJ family phage tail tip protein -
  A6L31_RS01240 (A6L31_01240) - 218663..219385 (-) 723 WP_002220716.1 tail assembly protein -
  A6L31_RS01245 (A6L31_01245) - 219382..220137 (-) 756 WP_002244525.1 C40 family peptidase -
  A6L31_RS01250 (A6L31_01250) - 220134..220856 (-) 723 WP_002217456.1 phage minor tail protein L -
  A6L31_RS01255 (A6L31_01255) - 220916..221185 (-) 270 WP_002217457.1 phage tail protein -
  A6L31_RS01260 (A6L31_01260) - 221207..224425 (-) 3219 WP_002224445.1 phage tail length tape measure family protein -
  A6L31_RS01265 (A6L31_01265) - 224418..224690 (-) 273 WP_002220710.1 DUF1799 domain-containing protein -
  A6L31_RS01270 (A6L31_01270) - 224738..225049 (-) 312 WP_002220709.1 phage tail assembly chaperone -
  A6L31_RS01275 (A6L31_01275) - 225113..225754 (-) 642 WP_002220708.1 phage tail protein -
  A6L31_RS01280 (A6L31_01280) - 225786..226205 (-) 420 WP_002244524.1 hypothetical protein -
  A6L31_RS01285 (A6L31_01285) - 226186..226488 (-) 303 WP_002244523.1 head-tail joining protein -
  A6L31_RS01290 (A6L31_01290) - 226481..226708 (-) 228 WP_002220705.1 hypothetical protein -
  A6L31_RS01295 (A6L31_01295) - 226766..228658 (-) 1893 WP_002220704.1 phage major capsid protein -
  A6L31_RS01300 (A6L31_01300) - 228639..230201 (-) 1563 WP_002220703.1 phage portal protein -
  A6L31_RS01305 (A6L31_01305) - 230210..230464 (-) 255 WP_002220702.1 hypothetical protein -
  A6L31_RS01310 (A6L31_01310) - 230451..230837 (-) 387 WP_002220701.1 DUF3310 domain-containing protein -
  A6L31_RS01315 (A6L31_01315) - 230840..231049 (-) 210 WP_002220700.1 hypothetical protein -
  A6L31_RS01320 (A6L31_01320) - 231066..233912 (-) 2847 WP_002220699.1 primase-helicase family protein -
  A6L31_RS01325 (A6L31_01325) - 234027..234374 (-) 348 WP_002220698.1 hypothetical protein -
  A6L31_RS01330 (A6L31_01330) - 234774..235490 (+) 717 WP_002220696.1 LexA family transcriptional regulator -
  A6L31_RS01335 (A6L31_01335) - 235593..236018 (+) 426 WP_002220695.1 hypothetical protein -
  A6L31_RS01340 (A6L31_01340) - 236249..236449 (+) 201 WP_002219431.1 hypothetical protein -
  A6L31_RS01345 (A6L31_01345) - 236517..236876 (+) 360 WP_002220694.1 hypothetical protein -
  A6L31_RS01350 (A6L31_01350) - 236901..237755 (+) 855 WP_002220693.1 YfdQ family protein -
  A6L31_RS01355 (A6L31_01355) - 237828..238043 (+) 216 WP_002220692.1 hypothetical protein -
  A6L31_RS01360 (A6L31_01360) - 238079..238339 (+) 261 WP_002220690.1 NGO1622 family putative holin -
  A6L31_RS01365 (A6L31_01365) - 238336..238629 (+) 294 WP_002220689.1 hypothetical protein -
  A6L31_RS01370 (A6L31_01370) - 238632..238823 (+) 192 WP_002219435.1 type ISP restriction/modification enzyme -
  A6L31_RS01375 (A6L31_01375) - 238823..238963 (+) 141 WP_002220688.1 hypothetical protein -
  A6L31_RS01380 (A6L31_01380) - 238960..239169 (+) 210 WP_002220687.1 hypothetical protein -
  A6L31_RS01385 (A6L31_01385) - 239227..240144 (-) 918 WP_002220686.1 KilA-N domain-containing protein -
  A6L31_RS01390 (A6L31_01390) - 241012..241491 (-) 480 WP_002241413.1 DUF4760 domain-containing protein -
  A6L31_RS01405 (A6L31_01405) - 241927..242307 (-) 381 WP_002220683.1 type II toxin-antitoxin system PemK/MazF family toxin -
  A6L31_RS01410 (A6L31_01410) - 242462..243658 (-) 1197 WP_002224447.1 tyrosine-type recombinase/integrase -
  A6L31_RS01425 (A6L31_01425) yaaA 244190..244969 (-) 780 WP_002220681.1 peroxide stress protein YaaA -
  A6L31_RS14515 - 245018..245143 (-) 126 Protein_271 IS5/IS1182 family transposase -
  A6L31_RS01430 (A6L31_01430) dapC 245231..246418 (-) 1188 WP_002220680.1 succinyldiaminopimelate transaminase -
  A6L31_RS01435 (A6L31_01435) dut 246494..246946 (-) 453 WP_002220679.1 dUTP diphosphatase -
  A6L31_RS01440 (A6L31_01440) - 247089..247517 (+) 429 Protein_274 AzlC family ABC transporter permease -
  A6L31_RS01445 (A6L31_01445) pilX 249421..249894 (-) 474 WP_002248276.1 PilX family type IV pilin Machinery gene
  A6L31_RS01450 (A6L31_01450) pilK 249899..250492 (-) 594 WP_002248275.1 pilus assembly PilX family protein Machinery gene
  A6L31_RS01455 (A6L31_01455) pilJ 250471..251409 (-) 939 WP_002248274.1 PilW family protein Machinery gene
  A6L31_RS01460 (A6L31_01460) pilV 251406..252017 (-) 612 WP_002248273.1 type IV pilus modification protein PilV Machinery gene
  A6L31_RS01465 (A6L31_01465) pilH 252041..252718 (-) 678 WP_002248272.1 GspH/FimT family pseudopilin Machinery gene
  A6L31_RS01470 (A6L31_01470) dnaB 252873..254279 (-) 1407 WP_002248271.1 replicative DNA helicase -
  A6L31_RS01475 (A6L31_01475) - 254442..255029 (+) 588 WP_002220670.1 superoxide dismutase -
  A6L31_RS01480 (A6L31_01480) - 255601..256110 (-) 510 WP_002213852.1 isoprenylcysteine carboxyl methyltransferase family protein -
  A6L31_RS01485 (A6L31_01485) - 256442..256774 (-) 333 WP_002226445.1 hypothetical protein -
  A6L31_RS14520 - 256819..256926 (-) 108 Protein_284 IS5/IS1182 family transposase -
  A6L31_RS01490 (A6L31_01490) cysT 257065..257901 (+) 837 WP_002226446.1 sulfate ABC transporter permease subunit CysT -
  A6L31_RS01495 (A6L31_01495) cysW 258156..259016 (+) 861 WP_002220668.1 sulfate ABC transporter permease subunit CysW -
  A6L31_RS01500 (A6L31_01500) - 259013..260086 (+) 1074 WP_002220667.1 sulfate/molybdate ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 17528.29 Da        Isoelectric Point: 9.4344

>NTDB_id=191748 A6L31_RS01445 WP_002248276.1 249421..249894(-) (pilX) [Neisseria meningitidis strain M22191]
MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTEMVGINNISKQFILKNPLDDNQTIKSKLEIFVSGYKM
NPKIAEKYNVSVHFVNKEKPRAYSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAHLETLSSDVGCEAFSNRKK

Nucleotide


Download         Length: 474 bp        

>NTDB_id=191748 A6L31_RS01445 WP_002248276.1 249421..249894(-) (pilX) [Neisseria meningitidis strain M22191]
ATGGAACAAAAAGGGTTTACATTGATTGAGATGATGATAGTCGTCGCGATACTCGGCATCATCAGCGTCATTGCCATACC
TTCTTATCAAAGTTATATTGAAAAAGGCTATCAGTCCCAGCTTTATACGGAGATGGTCGGTATCAACAATATTTCCAAAC
AGTTTATTTTGAAAAATCCCCTGGACGATAATCAGACCATCAAGAGCAAACTGGAAATATTTGTCTCAGGCTATAAGATG
AATCCGAAAATTGCCGAAAAATATAATGTTTCGGTGCATTTTGTCAATAAGGAAAAACCAAGGGCATACAGCTTGGTCGG
CGTTCCAAAGACGGGGACGGGTTATACTTTGTCGGTATGGATGAACAGCGTGGGCGACGGATACAAATGCCGTGATGCCG
CTTCTGCCCGAGCCCATTTGGAGACCTTGTCCTCAGATGTCGGCTGTGAAGCCTTCTCTAATCGTAAAAAATAG

Domains

Predicted by InterproScan.

Minor type IV pilin, PilX (Pilin_PilX)

(31-157)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilX Neisseria meningitidis 8013

99.363

100

0.994
  pilL Neisseria gonorrhoeae MS11

86.624

100

0.866

Multiple sequence alignment