Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   A6M04_RS07345 Genome accession   NZ_CP016668
Coordinates   1424971..1425831 (+) Length   286 a.a.
NCBI ID   WP_002241067.1    Uniprot ID   A1KVV2
Organism   Neisseria meningitidis strain M25472     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 1423398..1424363 1424971..1425831 flank 608


Gene organization within MGE regions


Location: 1423398..1425831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6M04_RS07335 (A6M04_07335) - 1423398..1424363 (+) 966 WP_002216744.1 IS30-like element IS1655 family transposase -
  A6M04_RS07340 (A6M04_07340) pilG 1424391..1424897 (+) 507 Protein_1371 type 4 pilus assembly protein PilG -
  A6M04_RS07345 (A6M04_07345) pilD 1424971..1425831 (+) 861 WP_002241067.1 prepilin peptidase Machinery gene

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 31331.48 Da        Isoelectric Point: 9.1920

>NTDB_id=191069 A6M04_RS07345 WP_002241067.1 1424971..1425831(+) (pilD) [Neisseria meningitidis strain M25472]
MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKKHLNLPLTEKESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Nucleotide


Download         Length: 861 bp        

>NTDB_id=191069 A6M04_RS07345 WP_002241067.1 1424971..1425831(+) (pilD) [Neisseria meningitidis strain M25472]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCGGTGTTCGGGCTGCTGGTCGGCAGTTTCTTAAA
CGTCGTCATTTACCGCGTGCCGGTCATGATGGAACGCGGCTGGACGGTATTTGCCAAAAAACATTTAAACCTGCCGCTGA
CCGAAAAGGAAAGCCGTACCTTCAACCTGATGAAACCGGATTCCTGCTGTCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGCTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTATTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACATTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACACTGCCC
TTGATTTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATGGTTCGATATGGCTTTTATGCGCGGTATATAAACTGATTAAAGGCGAAATCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(134-242)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1KVV2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

96.14

99.65

0.958
  pilD Vibrio cholerae strain A1552

44.444

100

0.448
  pilD Acinetobacter baumannii D1279779

46.97

92.308

0.434
  pilD Acinetobacter nosocomialis M2

46.97

92.308

0.434
  pilD Vibrio campbellii strain DS40M4

44.565

96.503

0.43

Multiple sequence alignment