Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   B5M25_RS00020 Genome accession   NZ_CP021171
Coordinates   3912..5024 (+) Length   370 a.a.
NCBI ID   WP_000775113.1    Uniprot ID   P68863
Organism   Staphylococcus aureus strain CHU15-056     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..10024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5M25_RS00005 (B5M25_00005) dnaA 517..1878 (+) 1362 WP_001290433.1 chromosomal replication initiator protein DnaA -
  B5M25_RS00010 (B5M25_00010) dnaN 2156..3289 (+) 1134 WP_000969811.1 DNA polymerase III subunit beta -
  B5M25_RS00015 (B5M25_00015) yaaA 3670..3915 (+) 246 WP_001789359.1 S4 domain-containing protein YaaA -
  B5M25_RS00020 (B5M25_00020) recF 3912..5024 (+) 1113 WP_000775113.1 DNA replication/repair protein RecF Machinery gene
  B5M25_RS00025 (B5M25_00025) gyrB 5034..6968 (+) 1935 WP_000255578.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  B5M25_RS00030 (B5M25_00030) gyrA 7005..9674 (+) 2670 WP_000819096.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42415.43 Da        Isoelectric Point: 6.4362

>NTDB_id=190853 B5M25_RS00020 WP_000775113.1 3912..5024(+) (recF) [Staphylococcus aureus strain CHU15-056]
MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIYTLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGT
MPLTMFITKKGKQVKVNHLEQSRLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDKEALSLNYLPSLKFDYAQNEAAR
LEEIMSILSDNMQREKERGISLFGPHRDDISFDVNGMDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLS
ELDDSRQTHLLSTIQHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=190853 B5M25_RS00020 WP_000775113.1 3912..5024(+) (recF) [Staphylococcus aureus strain CHU15-056]
ATGAAGTTAAATACACTCCAATTAGAAAATTATCGTAACTATGATGAGGTTACGTTGAAATGTCATCCTGACGTGAATAT
CCTCATTGGAGAAAATGCACAAGGAAAGACAAATTTACTTGAATCAATTTATACCTTAGCTTTAGCAAAAAGTCATAGAA
CGAGTAATGATAAGGAACTCATACGTTTTAATGCTGATTATGCTAAAATAGAAGGTGAGCTTAGTTATAGACACGGCACG
ATGCCATTAACAATGTTTATAACTAAAAAAGGTAAACAAGTCAAAGTGAATCACTTAGAGCAAAGTCGTCTAACTCAATA
TATTGGACACCTCAATGTGGTTCTATTTGCGCCAGAAGATTTGAATATTGTAAAAGGCTCTCCTCAAATAAGACGACGCT
TTATAGATATGGAGTTGGGCCAAATTTCTGCTGTTTACTTAAATGATTTAGCTCAATACCAACGTATTTTAAAGCAAAAG
AATAATTACTTAAAGCAGTTACAATTAGGCCAAAAAAAGGACTTAACAATGTTGGAAGTATTAAATCAGCAGTTTGCTGA
ATATGCAATGAAAGTAACTGATAAACGTGCACATTTTATTCAAGAGCTAGAGTCGTTAGCTAAACCGATTCATGCTGGTA
TCACAAATGATAAAGAAGCGTTGTCGCTAAATTATTTACCTAGTCTTAAATTTGATTATGCTCAAAATGAAGCGGCACGA
CTTGAAGAAATTATGTCTATTCTTAGCGATAATATGCAAAGAGAAAAAGAACGAGGCATTAGCTTATTCGGACCACATCG
AGATGATATAAGTTTTGATGTGAATGGCATGGATGCTCAAACATATGGTTCTCAAGGACAGCAACGTACAACGGCTTTGT
CCATTAAATTAGCTGAAATTGAGTTAATGAATATCGAAGTTGGGGAATATCCCATCTTATTATTAGACGATGTACTCAGT
GAATTAGATGATTCGCGTCAAACGCATTTATTAAGTACGATTCAGCATAAAGTACAAACATTTGTCACTACGACATCTGT
AGATGGTATTGATCATGAAATCATGAATAACGCTAAATTGTATCGTATTAATCAAGGTGAAATTATAAAGTAA

Domains

Predicted by InterProScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(3-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P68863

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

58.649

100

0.586
  recF Streptococcus pneumoniae R6

49.33

100

0.497

Multiple sequence alignment