Detailed information NTG0190568

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: A6L35_RS08810
• Genome accession: NZ_CP016657
• Coordinates: 1683713..1684573 (-)
• Length: 286 a.a.
• NCBI ID: WP_002241067.1
• Uniprot ID: A1KVV2
• Organism: Neisseria meningitidis strain M22745
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1678713..1689573

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A6L35_RS08780 (A6L35_08780)	ispB	1679199..1680173 (+)	975	WP_002247958.1	octaprenyl diphosphate synthase	-
A6L35_RS08785 (A6L35_08785)	-	1680178..1680624 (+)	447	WP_002220271.1	DUF441 domain-containing protein	-
A6L35_RS08795 (A6L35_08795)	pilF	1681116..1682792 (+)	1677	WP_002221967.1	type IV-A pilus assembly ATPase PilB	Machinery gene
A6L35_RS08800 (A6L35_08800)	-	1682877..1683086 (-)	210	WP_002216401.1	DNA gyrase inhibitor YacG	-
A6L35_RS08805 (A6L35_08805)	coaE	1683079..1683711 (-)	633	WP_002216402.1	dephospho-CoA kinase	-
A6L35_RS08810 (A6L35_08810)	pilD	1683713..1684573 (-)	861	WP_002241067.1	prepilin peptidase	Machinery gene
A6L35_RS08815 (A6L35_08815)	pilG	1684647..1685879 (-)	1233	WP_002216405.1	type 4 pilus assembly protein PilG	Machinery gene
A6L35_RS08820 (A6L35_08820)	pgi	1686389..1688032 (+)	1644	WP_002247959.1	glucose-6-phosphate isomerase	-
A6L35_RS08825 (A6L35_08825)	-	1688002..1688232 (+)	231	WP_025462060.1	hypothetical protein	-
A6L35_RS08830 (A6L35_08830)	dapD	1688242..1689063 (+)	822	WP_002220281.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31331.48 Da
• Isoelectric Point: 9.1920

>NTDB_id=190568 A6L35_RS08810 WP_002241067.1 1683713..1684573(-) (pilD) [Neisseria meningitidis strain M22745]
MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKKHLNLPLTEKESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Nucleotide

• Length: 861 bp

>NTDB_id=190568 A6L35_RS08810 WP_002241067.1 1683713..1684573(-) (pilD) [Neisseria meningitidis strain M22745]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCGGTGTTCGGGCTGCTGGTCGGCAGTTTCTTAAA
CGTCGTCATTTACCGCGTGCCGGTCATGATGGAACGCGGCTGGACGGTATTTGCCAAAAAACATTTAAACCTGCCGCTGA
CCGAAAAGGAAAGCCGTACCTTCAACCTGATGAAACCGGATTCCTGCTGTCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGCTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTATTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACATTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACACTGCCC
TTGATTTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATGGTTCGATATGGCTTTTATGCGCGGTATATAAACTGATTAAAGGCGAAATCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (134-242)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A1KVV2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	96.14	99.65	0.958
pilD	Vibrio cholerae strain A1552	44.444	100	0.448
pilD	Acinetobacter baumannii D1279779	46.97	92.308	0.434
pilD	Acinetobacter nosocomialis M2	46.97	92.308	0.434
pilD	Vibrio campbellii strain DS40M4	44.565	96.503	0.43