Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   A6L25_RS11170 Genome accession   NZ_CP016652
Coordinates   2157750..2158610 (-) Length   286 a.a.
NCBI ID   WP_002241067.1    Uniprot ID   A1KVV2
Organism   Neisseria meningitidis strain M08001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2152750..2163610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6L25_RS11140 (A6L25_11140) ispB 2153236..2154210 (+) 975 WP_002247958.1 octaprenyl diphosphate synthase -
  A6L25_RS11145 (A6L25_11145) - 2154215..2154661 (+) 447 WP_002220271.1 DUF441 domain-containing protein -
  A6L25_RS11155 (A6L25_11155) pilF 2155153..2156829 (+) 1677 WP_002221967.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A6L25_RS11160 (A6L25_11160) - 2156914..2157123 (-) 210 WP_002216401.1 DNA gyrase inhibitor YacG -
  A6L25_RS11165 (A6L25_11165) coaE 2157116..2157748 (-) 633 WP_002216402.1 dephospho-CoA kinase -
  A6L25_RS11170 (A6L25_11170) pilD 2157750..2158610 (-) 861 WP_002241067.1 prepilin peptidase Machinery gene
  A6L25_RS11175 (A6L25_11175) pilG 2158684..2159916 (-) 1233 WP_002216405.1 type 4 pilus assembly protein PilG Machinery gene
  A6L25_RS11180 (A6L25_11180) pgi 2160426..2162069 (+) 1644 WP_002247959.1 glucose-6-phosphate isomerase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 31331.48 Da        Isoelectric Point: 9.1920

>NTDB_id=190339 A6L25_RS11170 WP_002241067.1 2157750..2158610(-) (pilD) [Neisseria meningitidis strain M08001]
MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKKHLNLPLTEKESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Nucleotide


Download         Length: 861 bp        

>NTDB_id=190339 A6L25_RS11170 WP_002241067.1 2157750..2158610(-) (pilD) [Neisseria meningitidis strain M08001]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCGGTGTTCGGGCTGCTGGTCGGCAGTTTCTTAAA
CGTCGTCATTTACCGCGTGCCGGTCATGATGGAACGCGGCTGGACGGTATTTGCCAAAAAACATTTAAACCTGCCGCTGA
CCGAAAAGGAAAGCCGTACCTTCAACCTGATGAAACCGGATTCCTGCTGTCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGCTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTATTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACATTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACACTGCCC
TTGATTTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATGGTTCGATATGGCTTTTATGCGCGGTATATAAACTGATTAAAGGCGAAATCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(134-242)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1KVV2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

96.14

99.65

0.958
  pilD Vibrio cholerae strain A1552

44.444

100

0.448
  pilD Acinetobacter baumannii D1279779

46.97

92.308

0.434
  pilD Acinetobacter nosocomialis M2

46.97

92.308

0.434
  pilD Vibrio campbellii strain DS40M4

44.565

96.503

0.43

Multiple sequence alignment