Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   A6B39_RS07825 Genome accession   NZ_CP016606
Coordinates   1640007..1640564 (-) Length   185 a.a.
NCBI ID   WP_176670417.1    Uniprot ID   -
Organism   Mannheimia granulomatis strain BJ1680.3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1635007..1645564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6B39_RS07810 (A6B39_07760) - 1636255..1636818 (+) 564 WP_176670414.1 nitroreductase family protein -
  A6B39_RS07815 (A6B39_07765) - 1636820..1637641 (+) 822 WP_176670415.1 SDR family NAD(P)-dependent oxidoreductase -
  A6B39_RS07820 (A6B39_07770) - 1637686..1640010 (-) 2325 WP_176670416.1 Tex family protein -
  A6B39_RS07825 (A6B39_07775) pilF 1640007..1640564 (-) 558 WP_176670417.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  A6B39_RS07830 (A6B39_07780) - 1641182..1641958 (-) 777 WP_176670418.1 MerR family transcriptional regulator -
  A6B39_RS07835 (A6B39_07785) - 1642106..1642861 (-) 756 WP_176670419.1 MipA/OmpV family protein -
  A6B39_RS07840 (A6B39_07790) - 1643220..1644377 (-) 1158 WP_176670420.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  A6B39_RS07845 (A6B39_07795) - 1644638..1645534 (+) 897 WP_176670421.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 185 a.a.        Molecular weight: 21151.14 Da        Isoelectric Point: 8.4247

>NTDB_id=189631 A6B39_RS07825 WP_176670417.1 1640007..1640564(-) (pilF) [Mannheimia granulomatis strain BJ1680.3]
MFVKFFRKMTACVAIVLLAACSSSQDSSPEPEFRRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDSQDYLPYSVLA
YYYQQTGDVENAEKAYQKALNLSENRPDVLNNYGTFLCKQGQFQQAYQQFEKAIQSPKPYYHQADSLANIILCAKKEPNP
QKVVQTLIELEKLDKLRANLLKETK

Nucleotide


Download         Length: 558 bp        

>NTDB_id=189631 A6B39_RS07825 WP_176670417.1 1640007..1640564(-) (pilF) [Mannheimia granulomatis strain BJ1680.3]
ATGTTTGTAAAATTTTTTAGAAAAATGACCGCTTGTGTTGCTATTGTGTTACTAGCAGCTTGTTCTTCTAGCCAAGACTC
TTCCCCTGAGCCTGAATTTAGACGCTCGGAAGCAGTGAAAGCCCGCATTAATCTTGCTCTAGCTTATTTGGAGCAAAATG
ATTTCCCGAAAGCGAAGCAGAATATTGATAAAGCCCTCGCTCACGACAGCCAAGATTACTTACCTTATTCTGTGCTTGCC
TACTATTACCAACAAACAGGCGATGTAGAGAATGCGGAAAAAGCCTATCAGAAAGCCTTAAATTTAAGCGAAAATCGCCC
TGATGTGCTGAATAATTACGGCACCTTTTTATGTAAGCAAGGTCAATTTCAACAGGCTTATCAACAATTTGAAAAAGCGA
TACAAAGCCCCAAACCTTATTACCACCAAGCAGACAGCTTAGCAAATATTATTCTGTGTGCAAAAAAAGAGCCGAATCCA
CAGAAAGTGGTACAAACACTTATAGAGCTTGAGAAATTAGACAAACTTCGAGCGAATTTATTGAAGGAAACGAAATGA

Domains

Predicted by InterproScan.

MalT-like TPR region (TPR_MalT)

(46-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

60.963

100

0.616
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.093

92.973

0.438

Multiple sequence alignment