Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   A6A11_RS03035 Genome accession   NZ_CP016605
Coordinates   629849..630385 (+) Length   178 a.a.
NCBI ID   WP_132021865.1    Uniprot ID   -
Organism   Bisgaardia hudsonensis strain M327/99/2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 624849..635385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6A11_RS03005 (A6A11_02980) - 624908..625537 (+) 630 WP_132021853.1 lysophospholipid acyltransferase family protein -
  A6A11_RS03010 (A6A11_02985) - 625539..626468 (+) 930 WP_132021855.1 phosphatidate cytidylyltransferase -
  A6A11_RS03015 (A6A11_02990) mog 626491..627069 (+) 579 WP_132021857.1 molybdopterin adenylyltransferase -
  A6A11_RS03020 (A6A11_02995) glnB 627093..627431 (+) 339 WP_132021859.1 nitrogen regulatory protein P-II -
  A6A11_RS03025 (A6A11_03000) - 627453..628502 (+) 1050 WP_132021861.1 AI-2E family transporter -
  A6A11_RS03030 (A6A11_03005) - 628698..629816 (+) 1119 WP_132021863.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  A6A11_RS03035 (A6A11_03010) pilF/pilF2 629849..630385 (+) 537 WP_132021865.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  A6A11_RS03040 (A6A11_03015) - 630517..631602 (+) 1086 WP_132021867.1 RodZ domain-containing protein -
  A6A11_RS03045 (A6A11_03020) ispG 631618..632721 (+) 1104 WP_176672783.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  A6A11_RS03050 (A6A11_03025) hisS 632734..634005 (+) 1272 WP_132021869.1 histidine--tRNA ligase -
  A6A11_RS03055 (A6A11_03030) - 634035..634640 (+) 606 WP_132021871.1 YfgM family protein -
  A6A11_RS03060 (A6A11_03035) - 634811..635134 (+) 324 WP_236824065.1 methyltransferase family protein -

Sequence

Protein


Download         Length: 178 a.a.        Molecular weight: 20361.01 Da        Isoelectric Point: 8.5823

>NTDB_id=189600 A6A11_RS03035 WP_132021865.1 629849..630385(+) (pilF/pilF2) [Bisgaardia hudsonensis strain M327/99/2]
MRYIKWISAIYFSFLLSACVSNTQSNNSFNKESAAKARVELALGYLSFHNFTQAKASLDKALNYAPNYFLVHSAFAYFYQ
LQGNIVEARKSYESSIELDDKKGDVKNNYGAFLCGQGEFDLAFQQFDNALKTDGYHNQIDTYENIILCAKAANNLNRYNE
YLILLKKVAPERVHRLSK

Nucleotide


Download         Length: 537 bp        

>NTDB_id=189600 A6A11_RS03035 WP_132021865.1 629849..630385(+) (pilF/pilF2) [Bisgaardia hudsonensis strain M327/99/2]
ATGCGGTACATTAAATGGATTAGTGCAATTTATTTTTCTTTTTTATTATCCGCTTGTGTATCAAATACACAATCTAATAA
TAGCTTTAATAAAGAATCTGCAGCAAAAGCGAGGGTAGAGTTAGCACTTGGATACTTATCCTTTCATAACTTTACTCAAG
CAAAAGCAAGTTTAGATAAGGCTCTTAATTATGCGCCAAATTATTTTTTAGTTCATTCCGCATTTGCTTATTTCTATCAA
TTACAAGGTAATATAGTGGAAGCTCGGAAATCTTATGAAAGCTCAATTGAGTTAGATGATAAAAAAGGTGATGTGAAAAA
TAATTATGGTGCTTTTTTATGTGGTCAAGGTGAGTTTGATTTAGCGTTTCAACAATTTGATAATGCTTTAAAAACTGATG
GTTATCATAATCAAATTGATACCTATGAAAATATTATACTATGTGCTAAAGCTGCAAATAATCTAAATCGTTATAATGAA
TATTTAATATTGTTAAAGAAAGTCGCACCAGAGAGAGTTCATCGTTTATCTAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

48.864

98.876

0.483
  pilF Glaesserella parasuis strain SC1401

37.705

100

0.388

Multiple sequence alignment