Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BCY75_RS08225 Genome accession   NZ_CP016602
Coordinates   1627095..1627766 (-) Length   223 a.a.
NCBI ID   WP_004270751.1    Uniprot ID   A0A1B2A7J9
Organism   Latilactobacillus curvatus strain WiKim52     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1622095..1632766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCY75_RS08200 (BCY75_08200) rpsO 1622409..1622678 (-) 270 WP_004271112.1 30S ribosomal protein S15 -
  BCY75_RS08205 (BCY75_08205) rpsT 1622955..1623209 (+) 255 WP_004271117.1 30S ribosomal protein S20 -
  BCY75_RS08210 (BCY75_08210) holA 1623264..1624292 (-) 1029 WP_065825721.1 DNA polymerase III subunit delta -
  BCY75_RS08215 (BCY75_08215) comEC 1624293..1626407 (-) 2115 WP_231118784.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BCY75_RS08220 (BCY75_08220) - 1626548..1627036 (-) 489 WP_004270740.1 ComE operon protein 2 -
  BCY75_RS08225 (BCY75_08225) comEA 1627095..1627766 (-) 672 WP_004270751.1 helix-hairpin-helix domain-containing protein Machinery gene
  BCY75_RS08230 (BCY75_08230) - 1627843..1628889 (-) 1047 WP_081303852.1 SepM family pheromone-processing serine protease -
  BCY75_RS08235 (BCY75_08235) coaD 1628891..1629385 (-) 495 WP_065825725.1 pantetheine-phosphate adenylyltransferase -
  BCY75_RS08240 (BCY75_08240) rsmD 1629397..1629951 (-) 555 WP_065825726.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  BCY75_RS08245 (BCY75_08245) - 1629957..1630247 (-) 291 WP_004270748.1 YlbG family protein -
  BCY75_RS08250 (BCY75_08250) - 1630267..1631355 (-) 1089 WP_065825727.1 CAP-associated domain-containing protein -
  BCY75_RS08255 (BCY75_08255) - 1631368..1632555 (-) 1188 WP_004270744.1 FtsW/RodA/SpoVE family cell cycle protein -

Sequence

Protein


Download         Length: 223 a.a.        Molecular weight: 24849.51 Da        Isoelectric Point: 9.9048

>NTDB_id=189564 BCY75_RS08225 WP_004270751.1 1627095..1627766(-) (comEA) [Latilactobacillus curvatus strain WiKim52]
MWRDYWQRYRRFWWVPCLVIILLGGGVFWQHSRQQRFLSTNQQLMAQSTKRKVKAKPVKQKKSVSKGGFVDIKGAVAHPG
IYPICVAKTRLFEVLKQAGDVTEAADTSQLNLAQTLSDQLIIYVPTKGEAVQAEELVKAADEPQAATDNQAGASPISTDA
KINLNQADATQLQTLNGIGPKKAEQIIAYRDEQGQFKQIEDLQKVGGIGPKTFEQLRPQICVN

Nucleotide


Download         Length: 672 bp        

>NTDB_id=189564 BCY75_RS08225 WP_004270751.1 1627095..1627766(-) (comEA) [Latilactobacillus curvatus strain WiKim52]
ATGTGGCGCGACTATTGGCAACGGTATCGTCGATTCTGGTGGGTACCTTGTTTGGTGATAATTTTACTTGGTGGTGGTGT
GTTTTGGCAGCATAGTCGGCAACAACGTTTTCTATCAACCAATCAACAATTGATGGCGCAATCAACTAAGCGAAAAGTGA
AGGCAAAACCCGTCAAACAGAAGAAAAGCGTCTCAAAAGGTGGGTTTGTCGATATCAAAGGCGCTGTGGCACATCCAGGG
ATTTATCCGATTTGTGTTGCAAAGACGCGTTTATTTGAGGTGCTGAAACAAGCGGGGGATGTGACCGAAGCAGCGGATAC
GAGTCAGTTGAACTTAGCGCAAACATTGAGTGACCAACTTATTATCTATGTGCCAACTAAGGGAGAAGCGGTGCAAGCCG
AAGAATTAGTGAAAGCGGCAGACGAGCCACAGGCTGCGACTGACAATCAAGCGGGAGCCAGTCCGATATCAACTGATGCT
AAAATCAACCTAAACCAAGCGGATGCGACACAATTGCAAACGCTAAATGGCATCGGTCCTAAAAAAGCTGAACAAATAAT
TGCATATCGGGATGAGCAAGGGCAGTTTAAACAAATCGAAGATCTTCAAAAAGTTGGGGGAATTGGGCCGAAAACGTTTG
AACAATTACGGCCACAAATTTGTGTTAATTAG

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(70-115)

Helix-hairpin-helix motif (HHH_3)

(160-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B2A7J9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

64.53

100

0.677
  comEA Lactococcus lactis subsp. cremoris KW2

36.283

100

0.368

Multiple sequence alignment