Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BCM43_RS07690 Genome accession   NZ_CP016588
Coordinates   1480827..1481870 (+) Length   347 a.a.
NCBI ID   WP_001013186.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain KNU-07     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1475827..1486870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCM43_RS07655 (BCM43_07655) - 1476195..1476683 (+) 489 WP_000764267.1 hypothetical protein -
  BCM43_RS07660 (BCM43_07660) - 1476718..1477029 (-) 312 WP_001093237.1 hypothetical protein -
  BCM43_RS07665 (BCM43_07665) - 1477282..1477497 (+) 216 WP_001008326.1 DUF3912 family protein -
  BCM43_RS07670 (BCM43_07670) - 1477585..1477977 (+) 393 WP_000875390.1 hypothetical protein -
  BCM43_RS07675 (BCM43_07675) - 1478057..1479451 (-) 1395 WP_001094334.1 L-cystine transporter -
  BCM43_RS07680 (BCM43_07680) - 1479558..1479800 (-) 243 WP_000440721.1 DUF2626 domain-containing protein -
  BCM43_RS07685 (BCM43_07685) - 1479926..1480621 (-) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  BCM43_RS07690 (BCM43_07690) comGA 1480827..1481870 (+) 1044 WP_001013186.1 competence type IV pilus ATPase ComGA Machinery gene
  BCM43_RS07695 (BCM43_07695) comGB 1481863..1482894 (+) 1032 WP_000475192.1 competence type IV pilus assembly protein ComGB -
  BCM43_RS07700 (BCM43_07700) comGC 1482906..1483205 (+) 300 WP_001178686.1 competence type IV pilus major pilin ComGC -
  BCM43_RS07705 (BCM43_07705) comGD 1483202..1483657 (+) 456 WP_000813142.1 competence type IV pilus minor pilin ComGD -
  BCM43_RS07710 (BCM43_07710) - 1483650..1483952 (+) 303 WP_065703188.1 competence protein ComG -
  BCM43_RS07715 (BCM43_07715) comGF 1483922..1484392 (+) 471 WP_000923099.1 competence type IV pilus minor pilin ComGF -
  BCM43_RS07720 (BCM43_07720) comGG 1484389..1484760 (+) 372 WP_001231273.1 competence type IV pilus minor pilin ComGG -
  BCM43_RS07725 (BCM43_07725) - 1484937..1485587 (+) 651 WP_000183862.1 2OG-Fe(II) oxygenase -
  BCM43_RS07730 (BCM43_07730) - 1485703..1486200 (+) 498 WP_000836650.1 shikimate kinase -
  BCM43_RS07735 (BCM43_07735) - 1486239..1486439 (+) 201 WP_000106083.1 YqzE family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39114.45 Da        Isoelectric Point: 8.3536

>NTDB_id=189418 BCM43_RS07690 WP_001013186.1 1480827..1481870(+) (comGA) [Bacillus thuringiensis strain KNU-07]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTSQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=189418 BCM43_RS07690 WP_001013186.1 1480827..1481870(+) (comGA) [Bacillus thuringiensis strain KNU-07]
ATGAATGGGATTGAAATTTTCGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGCCAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGCTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAAGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGGGATGAGGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGATATGAGTTAAAACAAGCACTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCATCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369

Multiple sequence alignment