Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   A4W75_RS05700 Genome accession   NZ_CP016467
Coordinates   1094014..1095015 (-) Length   333 a.a.
NCBI ID   WP_004270906.1    Uniprot ID   A0A1B2A6B9
Organism   Latilactobacillus curvatus strain TMW 1.27     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1089014..1100015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W75_RS05660 (A4W75_05475) - 1089379..1089831 (-) 453 WP_056967146.1 hypothetical protein -
  A4W75_RS05665 (A4W75_05480) - 1089891..1091084 (-) 1194 WP_004270911.1 acetate/propionate family kinase -
  A4W75_RS05670 (A4W75_05485) - 1091106..1092116 (-) 1011 WP_054644024.1 class I SAM-dependent methyltransferase -
  A4W75_RS05675 (A4W75_05490) - 1092241..1092564 (-) 324 WP_039099486.1 hypothetical protein -
  A4W75_RS05680 (A4W75_05495) - 1092542..1092922 (-) 381 WP_004270914.1 ComGF family competence protein -
  A4W75_RS11010 - 1092944..1093027 (-) 84 Protein_1128 type II secretion system protein -
  A4W75_RS05685 (A4W75_05500) - 1092993..1093298 (-) 306 WP_056965894.1 hypothetical protein -
  A4W75_RS05690 (A4W75_05505) - 1093285..1093644 (-) 360 WP_158022155.1 hypothetical protein -
  A4W75_RS05695 (A4W75_05510) comGC 1093709..1094017 (-) 309 WP_004270901.1 competence type IV pilus major pilin ComGC Machinery gene
  A4W75_RS05700 (A4W75_05515) comGB 1094014..1095015 (-) 1002 WP_004270906.1 type II secretion system F family protein Machinery gene
  A4W75_RS05705 (A4W75_05520) comGA 1095005..1095724 (-) 720 WP_253732251.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  A4W75_RS05710 (A4W75_05530) - 1095983..1096714 (-) 732 WP_004270897.1 YebC/PmpR family DNA-binding transcriptional regulator -
  A4W75_RS05715 (A4W75_05535) - 1096804..1097304 (-) 501 WP_004270893.1 VanZ family protein -
  A4W75_RS05720 (A4W75_05540) - 1097407..1097784 (+) 378 WP_111447553.1 hypothetical protein -
  A4W75_RS05730 (A4W75_05550) - 1097965..1098300 (-) 336 WP_136903898.1 hypothetical protein -
  A4W75_RS05735 (A4W75_05555) - 1098495..1098734 (+) 240 WP_004270892.1 cytochrome b5 domain-containing protein -

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 37875.07 Da        Isoelectric Point: 9.6278

>NTDB_id=188453 A4W75_RS05700 WP_004270906.1 1094014..1095015(-) (comGB) [Latilactobacillus curvatus strain TMW 1.27]
MKTKTTVQFNTEFMLLLGRLLQNGFSLQQALEFMPIVFPTQKQWLHQVQTGLEAGKRLAPSLAAVSFPTHLVAQIELTEL
QGNLGECLLHLGQLQRIQQKRRRELVGVIAYPCFLLLFLGALIGMMQIYLFPEIAQFNPTTSATSPLQLFFRVMGTFVIM
AGGLISVYLFRWHRRPLLQRLAAAFRWPFIGKTLQAYYQYCLLFDLATCLNNGLNLAEMHQLTHRLAKTSWLVQLMQQLE
TAVQKGGTLLANLADGVFYPPELQLVLAKGSPLQQMAKEVDLLAVIKYDELQRQLKQKVNWLQPLLFILIGIIIICTYLS
ILLPLYHTMEGIS

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=188453 A4W75_RS05700 WP_004270906.1 1094014..1095015(-) (comGB) [Latilactobacillus curvatus strain TMW 1.27]
ATGAAGACTAAAACCACTGTTCAATTCAATACTGAATTTATGTTGCTGTTAGGGCGCTTACTGCAAAATGGTTTTTCTTT
ACAACAAGCGCTTGAGTTCATGCCGATTGTTTTTCCGACACAAAAGCAGTGGCTGCATCAGGTGCAAACTGGGTTAGAAG
CAGGAAAACGACTAGCACCAAGCTTAGCGGCGGTTTCGTTTCCAACACACCTTGTTGCACAGATTGAATTGACTGAGTTG
CAGGGAAACTTAGGCGAGTGTTTGTTACATCTTGGCCAATTACAGCGAATTCAACAGAAACGGCGTAGAGAGTTAGTTGG
CGTAATTGCCTATCCCTGTTTTCTACTCCTTTTTCTCGGGGCGTTGATTGGGATGATGCAGATTTATCTTTTTCCGGAAA
TCGCGCAGTTTAACCCAACAACTTCGGCGACATCACCATTACAACTATTTTTTAGAGTGATGGGGACATTCGTTATTATG
GCTGGTGGTCTGATTAGTGTGTATTTATTCCGTTGGCATCGGCGACCGCTATTACAACGATTGGCGGCGGCATTTAGATG
GCCGTTCATCGGTAAAACGTTGCAGGCTTACTATCAGTATTGCTTATTATTTGATTTAGCGACTTGCCTGAATAACGGCT
TAAACTTGGCCGAAATGCATCAGCTTACACATCGTTTGGCGAAAACATCGTGGCTTGTACAATTAATGCAACAACTTGAA
ACGGCTGTCCAAAAGGGGGGCACGCTATTAGCGAACTTAGCGGATGGCGTTTTTTATCCACCTGAGCTGCAATTAGTGCT
CGCTAAGGGAAGTCCGTTACAACAGATGGCCAAGGAGGTTGATCTGCTTGCAGTCATTAAATACGATGAACTGCAACGGC
AATTGAAACAGAAGGTGAATTGGCTACAACCGCTCTTGTTTATCCTAATTGGGATCATCATTATTTGTACCTATCTCAGT
ATTTTATTACCGCTATATCATACAATGGAGGGAATTTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(13-132)

Type II secretion system (T2SS), protein F (T2SSF)

(205-324)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B2A6B9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Latilactobacillus sakei subsp. sakei 23K

57.27

100

0.58

Multiple sequence alignment