Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   A4W81_RS05570 Genome accession   NZ_CP016465
Coordinates   1097841..1098548 (-) Length   235 a.a.
NCBI ID   WP_232320763.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain TMW 1.3     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1092841..1103548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W81_RS05545 (A4W81_05440) rpsO 1093138..1093407 (-) 270 WP_011374766.1 30S ribosomal protein S15 -
  A4W81_RS05550 (A4W81_05445) rpsT 1093686..1093940 (+) 255 WP_011374767.1 30S ribosomal protein S20 -
  A4W81_RS05555 (A4W81_05450) holA 1094009..1095037 (-) 1029 WP_011374768.1 DNA polymerase III subunit delta -
  A4W81_RS05560 (A4W81_05455) comEC 1095099..1097141 (-) 2043 WP_337962200.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  A4W81_RS05565 (A4W81_05460) - 1097295..1097783 (-) 489 WP_011374770.1 ComE operon protein 2 -
  A4W81_RS05570 (A4W81_05465) comEA 1097841..1098548 (-) 708 WP_232320763.1 helix-hairpin-helix domain-containing protein Machinery gene
  A4W81_RS05575 (A4W81_05470) - 1098620..1099666 (-) 1047 WP_337962201.1 SepM family pheromone-processing serine protease -
  A4W81_RS05580 (A4W81_05475) coaD 1099668..1100165 (-) 498 WP_011374773.1 pantetheine-phosphate adenylyltransferase -
  A4W81_RS05585 (A4W81_05480) rsmD 1100178..1100732 (-) 555 WP_011374774.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  A4W81_RS05590 (A4W81_05485) - 1100737..1101027 (-) 291 WP_076632420.1 YlbG family protein -
  A4W81_RS05595 (A4W81_05490) - 1101051..1102070 (-) 1020 WP_337962202.1 CAP-associated domain-containing protein -
  A4W81_RS05600 (A4W81_05495) - 1102149..1103335 (-) 1187 Protein_1082 FtsW/RodA/SpoVE family cell cycle protein -

Sequence

Protein


Download         Length: 235 a.a.        Molecular weight: 26174.78 Da        Isoelectric Point: 9.7682

>NTDB_id=188418 A4W81_RS05570 WP_232320763.1 1097841..1098548(-) (comEA) [Latilactobacillus sakei strain TMW 1.3]
MMWQDYWQRYRRFWWVPCLVLLGIIAGGFYWHQSQQKQKVTQNEQLLATSASKTKPATKKTHQAATKKKGGFVDIKGAVV
YPGIYPITVSETRLFEVLKNAGGPTDAADTKRLNLAQKLTDQLIIYIPKKGEVVTDQELIQAPQQIATTSERTQEQVSTG
TSANASSTSDTAKIDLNQADSTQLQTLNGIGPKKAEQIIAYRDEQGKFKQIEDLQKVGGIGPKTFEQLQSQICVN

Nucleotide


Download         Length: 708 bp        

>NTDB_id=188418 A4W81_RS05570 WP_232320763.1 1097841..1098548(-) (comEA) [Latilactobacillus sakei strain TMW 1.3]
ATGATGTGGCAAGATTATTGGCAGCGATATCGGCGTTTTTGGTGGGTGCCTTGCTTAGTGCTATTGGGGATCATCGCGGG
TGGCTTTTATTGGCATCAATCGCAACAAAAACAAAAAGTAACGCAAAATGAGCAACTGCTAGCAACCAGTGCGTCTAAAA
CAAAACCCGCTACTAAGAAGACACACCAAGCGGCAACCAAGAAAAAAGGCGGTTTTGTCGATATAAAAGGCGCGGTGGTC
TACCCGGGAATTTATCCGATCACAGTCTCTGAAACGCGCTTATTTGAGGTGCTCAAAAATGCAGGTGGTCCGACTGATGC
GGCTGATACCAAGCGGCTTAATTTAGCTCAAAAGTTAACCGATCAACTGATTATTTATATTCCGAAAAAAGGAGAGGTGG
TTACTGATCAAGAACTCATTCAAGCGCCACAACAAATCGCAACGACCAGTGAGCGTACGCAAGAACAGGTATCAACTGGG
ACAAGTGCGAATGCATCGAGCACGAGTGACACTGCTAAAATCGATTTGAATCAGGCTGATAGTACGCAGCTTCAGACCCT
CAATGGCATTGGCCCCAAGAAAGCTGAACAAATAATTGCTTATCGTGATGAACAAGGAAAATTTAAACAGATTGAAGATC
TACAAAAAGTCGGCGGGATTGGTCCGAAGACGTTTGAACAATTGCAATCACAAATTTGTGTGAATTAG

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(172-233)

SLBB domain (SLBB)

(73-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

99.574

100

0.996
  comEA Lactococcus lactis subsp. cremoris KW2

36.957

97.872

0.362

Multiple sequence alignment