Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BSY238_RS07985 Genome accession   NZ_CP016448
Coordinates   1723523..1724959 (-) Length   478 a.a.
NCBI ID   WP_069038664.1    Uniprot ID   A0A1B3LRS7
Organism   Methyloversatilis sp. RAC08     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1718523..1729959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSY238_RS07955 (BSY238_1611) - 1719454..1720251 (-) 798 WP_150123993.1 hypothetical protein -
  BSY238_RS07960 (BSY238_1612) - 1720368..1721054 (-) 687 WP_083223975.1 hypothetical protein -
  BSY238_RS07965 (BSY238_1613) - 1721196..1721522 (-) 327 WP_069038660.1 PsiF family protein -
  BSY238_RS07970 (BSY238_1614) hprK 1721626..1722561 (-) 936 WP_069038661.1 HPr(Ser) kinase/phosphatase -
  BSY238_RS07975 (BSY238_1615) ptsN 1722530..1723018 (-) 489 WP_069038662.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BSY238_RS07980 (BSY238_1616) raiA 1723139..1723459 (-) 321 WP_069038663.1 ribosome-associated translation inhibitor RaiA -
  BSY238_RS07985 (BSY238_1617) rpoN/rpoN1 1723523..1724959 (-) 1437 WP_069038664.1 RNA polymerase factor sigma-54 Machinery gene
  BSY238_RS07990 (BSY238_1618) lptB 1724980..1725720 (-) 741 WP_069038665.1 LPS export ABC transporter ATP-binding protein -
  BSY238_RS07995 (BSY238_1619) recX 1725713..1726156 (-) 444 WP_069038666.1 recombination regulator RecX -
  BSY238_RS08000 (BSY238_1620) recA 1726153..1727184 (-) 1032 WP_069038667.1 recombinase RecA Machinery gene
  BSY238_RS08005 (BSY238_1621) - 1727241..1727735 (-) 495 WP_069038668.1 CinA family protein -
  BSY238_RS08010 (BSY238_1622) - 1727735..1728181 (-) 447 WP_083224136.1 phosphatidylglycerophosphatase A -
  BSY238_RS08015 (BSY238_1623) thiL 1728240..1729202 (-) 963 WP_069038670.1 thiamine-phosphate kinase -
  BSY238_RS08020 (BSY238_1624) nusB 1729223..1729714 (-) 492 WP_069038671.1 transcription antitermination factor NusB -

Sequence

Protein


Download         Length: 478 a.a.        Molecular weight: 53385.34 Da        Isoelectric Point: 4.6337

>NTDB_id=188339 BSY238_RS07985 WP_069038664.1 1723523..1724959(-) (rpoN/rpoN1) [Methyloversatilis sp. RAC08]
MKQSLQLKLSQHLALTPQLQQSIRLLQLSTIEFNQEIERFLDENPMLERDEPDATASFQPPPESVAEVRDAPQESTQASA
DERAPEGTGSADMDEWSGEGGNYGTRSGGDDDDGDYQDVQAAGTSLRDHLCGQLALSQMSERDRYLIRLLIEALDDDGYL
NQGLDELVELLPEEADIDLDDLSIALRQLQNFDPPGVAARDPRECLLMQLNFLATDEVSTLARVIIDQHIDLLAARDFAK
IRRAVGCDEELLRAAQNLIRSLNPRPGAQFAPIDARYIVPDVVVRKVRGQWTALLNPDAMPRLRINRLYAEILQGQRGSG
LSGQLQEAKWLIKNVQQRFDTILRVSQAIVERQRQFFEFGEVAMRPLTLREIAETLELHESTISRVTSQKYMATTRGLFE
LKYFFGSHVATESGGACSATAIRALIRQLVGAEDAKRPLSDSRIAEILGQQGIVVARRTIAKYRESLNIPPVSVRKAI

Nucleotide


Download         Length: 1437 bp        

>NTDB_id=188339 BSY238_RS07985 WP_069038664.1 1723523..1724959(-) (rpoN/rpoN1) [Methyloversatilis sp. RAC08]
ATGAAGCAGTCGCTGCAACTCAAACTTTCCCAGCATCTTGCGCTGACACCGCAGCTGCAACAGTCGATCCGGCTATTGCA
GCTGTCGACCATCGAATTCAATCAGGAAATCGAACGTTTCCTCGATGAGAACCCGATGCTCGAGCGTGACGAGCCGGATG
CCACCGCCAGCTTCCAGCCGCCGCCCGAAAGCGTTGCCGAGGTGCGCGATGCGCCGCAGGAAAGCACCCAGGCCAGCGCC
GACGAACGTGCCCCCGAGGGTACGGGCAGCGCCGACATGGACGAGTGGTCGGGCGAAGGCGGCAACTACGGTACGCGCAG
CGGCGGCGACGATGACGATGGCGACTACCAGGACGTGCAGGCGGCCGGTACCTCGCTGAGGGACCACCTGTGCGGCCAGC
TCGCCCTGTCGCAGATGAGCGAGCGCGACCGCTATCTGATCCGCCTGCTGATCGAAGCGCTTGACGATGACGGCTACCTG
AACCAGGGCCTGGACGAACTGGTCGAGCTGCTGCCGGAAGAGGCCGACATCGACCTCGACGACCTGTCGATCGCGCTGCG
CCAGTTGCAGAACTTCGACCCGCCCGGCGTGGCGGCGCGCGACCCGCGCGAATGCCTGCTGATGCAGCTCAACTTCCTGG
CGACCGACGAAGTATCGACGCTTGCACGCGTCATCATCGATCAGCACATCGATCTGCTGGCTGCGCGTGATTTCGCCAAG
ATCCGGCGTGCGGTCGGCTGCGATGAAGAACTGCTGCGCGCCGCACAGAACCTGATCCGTTCGCTCAACCCCCGTCCGGG
TGCCCAGTTCGCGCCGATCGATGCGCGCTACATCGTGCCCGACGTCGTGGTGCGCAAGGTGCGCGGCCAATGGACGGCGC
TGCTCAATCCGGATGCCATGCCGCGTCTGCGGATCAACCGGCTGTATGCGGAAATCCTGCAGGGCCAGCGCGGCAGCGGT
CTGTCCGGCCAGCTGCAGGAGGCCAAGTGGCTGATCAAGAATGTCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCTCA
GGCCATCGTCGAGCGCCAGCGCCAGTTCTTCGAATTCGGTGAGGTTGCGATGCGGCCGCTGACGCTGCGCGAGATCGCGG
AGACGCTGGAACTGCATGAATCCACCATTTCTCGGGTGACGTCGCAGAAGTACATGGCGACGACACGCGGCCTGTTCGAA
CTGAAATATTTTTTCGGCAGCCATGTGGCAACCGAAAGCGGCGGGGCCTGCTCAGCCACCGCAATCCGCGCACTCATCCG
CCAGTTGGTGGGTGCCGAGGATGCCAAGCGTCCGTTGTCGGACTCGCGCATCGCGGAAATACTGGGTCAGCAGGGCATCG
TGGTTGCGCGGCGCACCATCGCCAAATACCGTGAATCGCTCAACATCCCGCCGGTCAGCGTGCGCAAGGCGATCTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-309)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(322-476)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B3LRS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

54.941

100

0.582

Multiple sequence alignment