Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BA894_RS13930 Genome accession   NZ_CP016351
Coordinates   3073120..3074136 (-) Length   338 a.a.
NCBI ID   WP_065297449.1    Uniprot ID   -
Organism   Vibrio natriegens strain CCUG 16374     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3068120..3079136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BA894_RS13905 (BA894_13905) aroK 3068981..3069499 (-) 519 WP_014233147.1 shikimate kinase AroK -
  BA894_RS13910 (BA894_13910) pilQ 3069688..3071391 (-) 1704 WP_014233148.1 type IV pilus secretin PilQ Machinery gene
  BA894_RS13915 (BA894_13915) pilP 3071470..3071985 (-) 516 WP_014233149.1 pilus assembly protein PilP Machinery gene
  BA894_RS13920 (BA894_13920) pilO 3071975..3072568 (-) 594 WP_020334615.1 type 4a pilus biogenesis protein PilO Machinery gene
  BA894_RS13925 (BA894_13925) pilN 3072561..3073136 (-) 576 WP_065297448.1 PilN domain-containing protein Machinery gene
  BA894_RS13930 (BA894_13930) pilM 3073120..3074136 (-) 1017 WP_065297449.1 type IV pilus assembly protein PilM Machinery gene
  BA894_RS13935 (BA894_13935) - 3074280..3076847 (+) 2568 WP_065297450.1 penicillin-binding protein 1A -
  BA894_RS13940 (BA894_13940) oxyR 3076911..3077819 (-) 909 WP_014233154.1 DNA-binding transcriptional regulator OxyR -
  BA894_RS13945 (BA894_13945) - 3077978..3078706 (+) 729 WP_065297451.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37695.84 Da        Isoelectric Point: 6.8317

>NTDB_id=187273 BA894_RS13930 WP_065297449.1 3073120..3074136(-) (pilM) [Vibrio natriegens strain CCUG 16374]
MDKRIVTGIDIGHNSLKAVVLKPNGEQFALLGYKEILLKDGIVAENNTINHQEIVNTLKQVKKDLPFSASRVAISVPDNA
VISKKLHIEQDLEENEIEFAVVQAFSHQSPFPVDELSLDFVRLNHEGGLRGPVSYQVFATRKDVVESRVEAIRKSGFKPI
LVEVHSQSLGQIWKLATERFPDKNNYGLLDVGSVSSSFTMFTEQNELFHKEFACGTGSIQCSVQESPLSGEEQAKTNQFN
RQMVDRVKRQIQLYTSINGSQNIKGLWLSGEGATTPMLAEELSHQLAVECELLNPLGLFEMNVSKRKRRAVEWQHFSTAA
GLAVRGIQWLGGHRATSN

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=187273 BA894_RS13930 WP_065297449.1 3073120..3074136(-) (pilM) [Vibrio natriegens strain CCUG 16374]
ATGGATAAGCGAATCGTTACAGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTCGTACTTAAACCCAATGGCGAGCA
ATTCGCCTTGTTGGGTTACAAAGAAATATTACTTAAGGATGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAATACCCTTAAACAGGTGAAAAAAGACCTGCCATTTAGTGCAAGCAGAGTGGCAATTTCGGTGCCTGATAATGCG
GTTATCAGCAAAAAACTGCACATCGAACAAGACCTTGAAGAGAACGAAATAGAGTTCGCGGTCGTGCAGGCATTTTCTCA
TCAATCTCCTTTCCCCGTAGATGAGTTGAGTCTGGACTTTGTCCGTCTTAATCATGAAGGAGGTCTGCGAGGGCCAGTTA
GCTACCAAGTTTTTGCGACACGTAAAGATGTCGTGGAGAGTCGGGTTGAAGCAATACGTAAATCCGGTTTTAAACCTATT
CTCGTCGAGGTCCATTCACAAAGCCTCGGGCAGATATGGAAACTGGCCACAGAACGTTTTCCAGATAAAAACAACTACGG
TTTGCTTGATGTTGGTTCTGTGTCGAGCTCTTTCACGATGTTCACCGAGCAAAACGAGCTATTTCATAAAGAATTTGCCT
GTGGAACAGGTTCCATTCAGTGCTCTGTGCAGGAAAGCCCTCTGTCCGGAGAAGAACAAGCCAAAACAAATCAATTCAAT
CGTCAGATGGTCGACCGCGTCAAACGCCAGATACAGCTTTATACGTCCATTAACGGATCACAAAACATCAAAGGTCTTTG
GTTATCCGGTGAAGGGGCAACAACGCCAATGCTGGCTGAGGAGCTATCTCATCAATTGGCGGTCGAGTGCGAGTTACTGA
ATCCTTTAGGCCTATTTGAAATGAATGTCTCTAAACGTAAGCGTCGAGCGGTGGAATGGCAGCACTTTTCAACCGCCGCT
GGCTTGGCGGTACGAGGTATTCAATGGTTGGGAGGTCATCGTGCTACATCGAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

75.444

100

0.754
  pilM Vibrio cholerae strain A1552

47.619

99.408

0.473

Multiple sequence alignment