Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BA893_RS14365 Genome accession   NZ_CP016349
Coordinates   3148023..3149039 (-) Length   338 a.a.
NCBI ID   WP_065300289.1    Uniprot ID   -
Organism   Vibrio natriegens strain CCUG 16373     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3143023..3154039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BA893_RS14340 (BA893_14340) aroK 3143628..3144146 (-) 519 WP_014233147.1 shikimate kinase AroK -
  BA893_RS14345 (BA893_14345) pilQ 3144335..3146038 (-) 1704 WP_014233148.1 type IV pilus secretin PilQ Machinery gene
  BA893_RS14350 (BA893_14350) pilP 3146373..3146888 (-) 516 WP_065300286.1 pilus assembly protein PilP Machinery gene
  BA893_RS14355 (BA893_14355) pilO 3146878..3147471 (-) 594 WP_065300287.1 type 4a pilus biogenesis protein PilO Machinery gene
  BA893_RS14360 (BA893_14360) pilN 3147464..3148039 (-) 576 WP_065300288.1 PilN domain-containing protein Machinery gene
  BA893_RS14365 (BA893_14365) pilM 3148023..3149039 (-) 1017 WP_065300289.1 type IV pilus biogenesis protein PilM Machinery gene
  BA893_RS14370 (BA893_14370) - 3149183..3151750 (+) 2568 WP_065300290.1 penicillin-binding protein 1A -
  BA893_RS14375 (BA893_14375) oxyR 3151814..3152722 (-) 909 WP_014233154.1 DNA-binding transcriptional regulator OxyR -
  BA893_RS14380 (BA893_14380) - 3152881..3153609 (+) 729 WP_065300291.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37708.92 Da        Isoelectric Point: 7.0221

>NTDB_id=187228 BA893_RS14365 WP_065300289.1 3148023..3149039(-) (pilM) [Vibrio natriegens strain CCUG 16373]
MDKRIVTGIDIGHNSLKAVVLKPNGEQFALLGYKEIILKDGIVAENNTINHQEIVNTLKQVKKDLPFGASRVAISVPDNS
VISKKLHIEQDLEENEIEFAVVQAFSHQSPFPVDELSLDFVRLNHEGGLRGPVSYQVFATRKDVVESRVEAIRKSGFKPV
LVEVHSQSLGQIWKLATERFPDKSQYGLLDVGSVSSSFTMFTEQNELFHKEFACGTGSIHCSVQESPLSGEEQAKTNQFN
RQMVDRVKRQIQLYTSINGSQNIKGLWLSGEGSTTPMLAEELSHQLAIECELLNPLGLFEMNVAKRKRRVAEWQHFSTAA
GLAVRGIQWLGGRRALSN

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=187228 BA893_RS14365 WP_065300289.1 3148023..3149039(-) (pilM) [Vibrio natriegens strain CCUG 16373]
ATGGATAAGCGAATCGTTACAGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTCGTACTTAAACCCAATGGCGAGCA
ATTCGCCTTGTTGGGTTACAAAGAAATAATACTTAAGGATGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAATACCCTTAAACAGGTGAAAAAAGACCTGCCATTTGGTGCAAGCAGAGTGGCAATTTCAGTGCCTGATAATTCG
GTTATCAGCAAAAAACTGCACATCGAACAAGACCTTGAAGAGAACGAAATAGAGTTCGCGGTCGTGCAGGCATTTTCTCA
TCAATCTCCTTTCCCCGTAGATGAGTTGAGTCTGGACTTTGTCCGTCTTAATCATGAAGGAGGCCTGCGAGGGCCAGTTA
GCTACCAAGTTTTTGCAACACGTAAAGATGTCGTGGAGAGTCGGGTTGAAGCAATACGTAAATCCGGTTTTAAACCTGTC
CTCGTCGAGGTCCATTCACAAAGCCTCGGGCAGATATGGAAACTGGCCACAGAACGTTTTCCAGATAAAAGTCAGTACGG
TTTGCTTGATGTTGGTTCTGTGTCGAGCTCTTTCACGATGTTCACCGAGCAAAACGAGCTATTTCATAAAGAATTTGCCT
GTGGAACAGGTTCCATTCATTGCTCTGTGCAGGAAAGCCCTCTGTCCGGAGAAGAGCAAGCCAAAACGAATCAATTCAAT
CGTCAGATGGTCGATCGTGTCAAACGTCAGATACAGCTTTATACGTCTATTAACGGGTCACAAAACATCAAAGGTCTTTG
GTTATCCGGTGAAGGGTCAACAACGCCAATGCTGGCTGAGGAGTTATCTCATCAATTGGCGATAGAGTGCGAGTTACTGA
ACCCGTTAGGCTTATTTGAAATGAACGTCGCTAAACGTAAGCGTCGAGTGGCAGAGTGGCAGCACTTTTCAACCGCCGCT
GGCTTGGCAGTACGAGGTATCCAATGGTTAGGAGGTCGCCGTGCTCTATCGAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

74.852

100

0.749
  pilM Vibrio cholerae strain A1552

47.633

100

0.476

Multiple sequence alignment