Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VSVS12_RS02200 Genome accession   NZ_CP016307
Coordinates   477797..479515 (+) Length   572 a.a.
NCBI ID   WP_156773132.1    Uniprot ID   -
Organism   Vibrio scophthalmi strain VS-12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 472797..484515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VSVS12_RS02180 (VSVS12_00436) pilM 475089..476030 (+) 942 WP_065429733.1 type IV pilus biogenesis protein PilM -
  VSVS12_RS02185 (VSVS12_00437) - 476050..476652 (+) 603 WP_065429734.1 PilN domain-containing protein -
  VSVS12_RS02190 (VSVS12_00438) - 476645..477235 (+) 591 WP_065429735.1 type 4a pilus biogenesis protein PilO -
  VSVS12_RS02195 (VSVS12_00439) - 477222..477800 (+) 579 WP_005597183.1 pilus assembly protein PilP -
  VSVS12_RS02200 (VSVS12_00440) pilQ 477797..479515 (+) 1719 WP_156773132.1 type IV pilus secretin PilQ Machinery gene
  VSVS12_RS02205 (VSVS12_00441) aroK 479680..480198 (+) 519 WP_005597180.1 shikimate kinase AroK -
  VSVS12_RS02210 (VSVS12_00442) aroB 480229..481332 (+) 1104 WP_065429737.1 3-dehydroquinate synthase -
  VSVS12_RS02215 (VSVS12_00443) - 481428..482900 (+) 1473 WP_065429738.1 SPOR domain-containing protein -
  VSVS12_RS02220 (VSVS12_00444) - 483025..483879 (+) 855 WP_005597170.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62483.18 Da        Isoelectric Point: 4.5466

>NTDB_id=186749 VSVS12_RS02200 WP_156773132.1 477797..479515(+) (pilQ) [Vibrio scophthalmi strain VS-12]
MIQGWKVTNQKRMRCLQVICVVIVALLTAQPQAWSMSNQLLSVDFRLDDTNRGKVMLQLSSDQLALDIQEDPQGLVIELF
DTSVDDALLSRFDVADFGNNVQTMEVFRLDDRVRVHIQIEGRFAYQHTLDGGLLELVVTEAESEGKHDLSMLSKASANKI
AINFQDISVRNVLQLIAEYNDFNLVVSDAVSGNLTLRLDDVPWPQVLDIILQVKGLDKRIEGNVVLVAPKAEFELREQQR
LENARMAEQLSELHSDIIAVNFANASDIAEMIGGSGAISMLSERGSITVDERTNALLIRDLPSHIEMVRSVVGSLDVPVK
QVQIEARIVTVNQGSLDELGVRWSLNSTTGNLGATLEGNLNQRGSDGLLSPGDNIDDFLNVNLAATAGHSSSLAFQVAKL
GSSTLLDLELSALQSESKAEVISSPRLITTNKKPAYIEQGTDIPYLESTSSGASSVSFKKAVLSLKVTPQITPDNRLILD
LSVTQDRPGEVVKVGTGEAVSINTQRIGTQVLVDNGETIVLGGIYQHSVTDTVDKVPLLGDIPYLGALFRRSYQRMGKSE
LLIFVTPKVVMQ

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=186749 VSVS12_RS02200 WP_156773132.1 477797..479515(+) (pilQ) [Vibrio scophthalmi strain VS-12]
ATGATTCAAGGATGGAAGGTGACTAATCAAAAGAGGATGAGATGCTTACAGGTTATCTGCGTTGTTATTGTTGCCCTGCT
CACGGCGCAGCCCCAAGCTTGGTCGATGAGCAATCAACTGCTTTCGGTTGATTTTCGTTTGGATGACACGAACCGCGGCA
AAGTGATGTTGCAGCTTAGCAGTGATCAACTCGCGCTGGATATTCAGGAGGATCCACAAGGTTTGGTGATTGAGCTGTTT
GATACCTCGGTTGATGATGCGCTTTTATCACGCTTTGACGTGGCTGACTTCGGTAACAATGTTCAAACCATGGAGGTGTT
TCGTTTGGACGACCGCGTGCGAGTGCACATTCAAATTGAGGGCCGCTTTGCTTATCAGCATACTCTTGATGGAGGTTTGT
TAGAGTTAGTGGTCACCGAGGCTGAAAGCGAGGGAAAACACGACCTGAGTATGCTGAGCAAAGCCTCTGCCAATAAGATC
GCGATTAATTTTCAAGACATTTCGGTGCGCAATGTCTTGCAGTTGATTGCTGAATATAATGATTTTAATTTAGTCGTTTC
AGATGCGGTGAGTGGCAATCTCACCTTGCGACTGGATGATGTGCCATGGCCACAAGTGCTAGACATTATCTTGCAAGTTA
AAGGGTTAGATAAACGAATCGAAGGTAATGTCGTTCTGGTGGCACCAAAAGCAGAATTCGAACTGCGCGAGCAGCAACGA
TTAGAGAATGCCCGTATGGCAGAGCAATTAAGCGAGTTGCATTCTGACATCATTGCCGTCAACTTTGCTAATGCCAGTGA
CATTGCAGAGATGATTGGCGGTAGTGGCGCCATCAGTATGTTGTCTGAACGTGGTTCGATTACCGTTGATGAGCGCACCA
ATGCGTTACTCATCCGCGATTTGCCAAGCCATATTGAGATGGTTCGCAGCGTGGTGGGTTCGCTTGATGTCCCTGTTAAG
CAAGTACAGATAGAAGCGCGCATTGTCACGGTTAACCAAGGCAGTTTGGATGAGCTTGGGGTGCGTTGGAGCTTAAATTC
AACCACCGGCAACCTTGGCGCTACGCTAGAGGGCAATCTCAATCAGCGAGGTTCGGATGGTCTGTTATCGCCAGGGGATA
ACATCGATGACTTTCTTAACGTTAATTTAGCCGCCACCGCGGGTCATTCATCAAGCCTTGCGTTCCAAGTGGCCAAGTTA
GGCTCCTCAACCTTGTTGGATTTAGAACTGTCGGCACTGCAAAGTGAATCGAAAGCAGAGGTCATTTCTAGCCCTCGCTT
GATCACCACCAATAAAAAACCAGCCTATATTGAGCAGGGCACAGATATTCCTTACCTTGAATCCACCTCAAGTGGTGCCA
GTAGTGTTTCATTTAAAAAAGCGGTGTTGAGTTTAAAAGTCACGCCACAAATTACGCCGGATAACCGATTGATTCTTGAT
TTGAGCGTGACCCAAGATCGACCGGGTGAAGTGGTCAAAGTGGGTACGGGAGAGGCGGTGTCGATTAATACTCAACGTAT
TGGTACACAAGTATTAGTTGATAACGGGGAGACCATCGTGCTGGGCGGGATATATCAACACAGTGTGACCGATACTGTTG
ATAAAGTTCCTCTGCTTGGCGACATCCCATATTTAGGCGCACTGTTTCGTCGCAGCTACCAACGAATGGGCAAAAGTGAG
CTGCTGATTTTCGTCACTCCCAAAGTTGTTATGCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(257-321)

Bacterial type II and III secretion system protein (Secretin)

(412-570)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

64.22

95.28

0.612
  pilQ Vibrio cholerae strain A1552

63.956

94.58

0.605
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

63.956

94.58

0.605
  pilQ Legionella pneumophila strain Lp02

41.606

95.804

0.399
  pilQ Legionella pneumophila strain ERS1305867

41.606

95.804

0.399
  pilQ Pseudomonas aeruginosa PAK

39.123

99.65

0.39
  pilQ Acinetobacter baumannii strain A118

35.126

100

0.365
  pilQ Acinetobacter baumannii D1279779

35.126

100

0.365

Multiple sequence alignment