Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   dqs_RS02540 Genome accession   NZ_CP016210
Coordinates   549170..550630 (-) Length   486 a.a.
NCBI ID   WP_011764239.1    Uniprot ID   -
Organism   Azoarcus olearius strain DQS-4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 544170..555630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  dqs_RS02515 (dqs_0509) - 544696..545502 (-) 807 WP_065339572.1 ABC transporter substrate-binding protein -
  dqs_RS02520 (dqs_0510) ilvA 545658..547178 (-) 1521 WP_011764235.1 threonine ammonia-lyase, biosynthetic -
  dqs_RS02525 (dqs_0511) hprK 547303..548244 (-) 942 WP_011764236.1 HPr(Ser) kinase/phosphatase -
  dqs_RS02530 (dqs_0512) ptsN 548213..548698 (-) 486 WP_011764237.1 PTS IIA-like nitrogen regulatory protein PtsN -
  dqs_RS02535 (dqs_0513) hpf 548832..549155 (-) 324 WP_011764238.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  dqs_RS02540 (dqs_0514) rpoN/rpoN1 549170..550630 (-) 1461 WP_011764239.1 RNA polymerase factor sigma-54 Machinery gene
  dqs_RS02545 (dqs_0515) lptB 550640..551362 (-) 723 WP_011764240.1 LPS export ABC transporter ATP-binding protein -
  dqs_RS02550 (dqs_0516) recX 551349..551798 (-) 450 WP_065339573.1 recombination regulator RecX -
  dqs_RS02555 (dqs_0517) recA 551821..552855 (-) 1035 WP_011764242.1 recombinase RecA Machinery gene
  dqs_RS02560 (dqs_0518) - 552980..553465 (-) 486 WP_179948008.1 CinA family protein -
  dqs_RS02565 (dqs_0519) - 553468..553950 (-) 483 WP_065339574.1 phosphatidylglycerophosphatase A -
  dqs_RS02570 (dqs_0520) thiL 553955..554965 (-) 1011 WP_236778724.1 thiamine-phosphate kinase -
  dqs_RS02575 (dqs_0521) - 555100..555381 (-) 282 WP_011764246.1 RnfH family protein -

Sequence

Protein


Download         Length: 486 a.a.        Molecular weight: 54033.96 Da        Isoelectric Point: 4.8528

>NTDB_id=186183 dqs_RS02540 WP_011764239.1 549170..550630(-) (rpoN/rpoN1) [Azoarcus olearius strain DQS-4]
MKPTLQLKLSQHLTLTPQLQQSIKLLQLSTLELNTEIERFLLENPMLEREEMDGGGHDFQAQLSSGTTTASTTTESSGES
GGEAESRADSAESGSFDEGMDWSSGSGSGSSSNRDDDDDVDFQEFQAAGISLRDHLDQQVALSPLSDRDRALVRFLIEAL
DDDGYLHQELEELLELLPPELDVELDDLCIALKHVQSLEPAGIGARTPQECLALQLRAMPAEPVRDLALQIVSQHLELLA
ERNFAKLKKLTGCDDDALRAAHALICRLDPHPGAQHSVEETRYVLPDVVVRKLRGRWTVQLNPEAMPRLRINQLYASILQ
QNRGQGGGLTSQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGEVAMRPLTLREIADQLELHESTVSRVTTQKFM
ATPRGVFELKYFFGSHVATDTGGAASSTAIRALIRQLVDAEDRKKPLSDARIAELLGQQGIVVARRTIAKYRESLNIPPV
SLRKTI

Nucleotide


Download         Length: 1461 bp        

>NTDB_id=186183 dqs_RS02540 WP_011764239.1 549170..550630(-) (rpoN/rpoN1) [Azoarcus olearius strain DQS-4]
ATGAAGCCCACGCTCCAACTCAAGCTCTCCCAGCACCTGACGCTGACGCCGCAGTTGCAGCAGTCGATCAAGCTGCTGCA
GCTCTCCACGCTCGAACTCAACACCGAGATCGAACGCTTCCTGCTCGAAAACCCGATGCTCGAGCGCGAGGAGATGGACG
GCGGCGGGCACGACTTCCAGGCGCAGCTCTCTTCCGGCACCACCACGGCCTCGACCACGACCGAAAGCAGTGGCGAAAGT
GGCGGCGAAGCGGAGTCGCGCGCCGACAGCGCGGAAAGCGGCAGCTTCGACGAGGGCATGGACTGGTCGTCCGGCTCGGG
CAGCGGCTCATCGAGCAACCGCGACGACGATGATGACGTCGATTTTCAGGAGTTCCAGGCCGCGGGCATCTCGCTGCGCG
ACCATCTCGACCAGCAGGTCGCGCTGTCTCCGCTGTCCGACCGCGACCGCGCGCTGGTGCGCTTCCTGATCGAGGCGCTC
GACGACGACGGCTACCTGCACCAGGAGCTGGAAGAGTTGCTCGAGCTGCTGCCGCCCGAACTCGATGTCGAACTCGACGA
CCTCTGCATCGCGCTGAAGCATGTGCAAAGCCTCGAGCCGGCCGGCATCGGCGCCCGCACCCCGCAGGAATGCCTCGCGC
TGCAGCTGCGCGCGATGCCGGCCGAGCCGGTGCGCGACCTCGCCCTGCAGATCGTCAGCCAGCACCTCGAACTGCTCGCC
GAGCGCAACTTCGCCAAGCTCAAGAAACTCACCGGTTGCGACGACGACGCGCTGCGCGCCGCGCACGCGCTGATCTGCCG
GCTCGATCCGCATCCCGGCGCGCAGCATTCGGTCGAGGAAACCCGCTACGTCCTGCCCGACGTCGTGGTGCGCAAGCTGC
GCGGGCGCTGGACGGTGCAACTCAACCCGGAAGCGATGCCGCGCCTGCGCATCAACCAGCTCTACGCCAGCATCCTGCAG
CAGAACCGGGGCCAGGGCGGCGGCCTGACCAGCCAGCTGCAGGAGGCGCGCTGGCTGATCAAGAACGTGCAGCAGCGCTT
CGACACCATCCTGCGTGTCTCCCAGGCCATCGTTGACCAGCAACGGCAGTTCTTCGATCATGGGGAGGTGGCCATGCGGC
CGCTTACACTCCGGGAGATCGCAGACCAGCTCGAGCTGCACGAATCCACCGTATCGCGCGTCACCACCCAGAAGTTCATG
GCGACCCCGCGCGGCGTGTTCGAACTCAAGTATTTCTTCGGCAGCCATGTCGCCACCGATACCGGTGGCGCGGCCTCCTC
CACGGCGATTCGCGCGCTGATTCGCCAGTTGGTGGATGCCGAGGACAGGAAGAAACCCCTGTCCGACGCCCGGATTGCCG
AATTGTTGGGCCAGCAGGGTATCGTGGTCGCCAGGCGGACCATCGCCAAGTATCGCGAATCGCTCAATATCCCGCCGGTC
AGTCTGCGCAAAACGATCTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(330-484)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(122-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

57.341

100

0.595

Multiple sequence alignment