Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   A9P85_RS03385 Genome accession   NZ_CP016029
Coordinates   723634..724083 (-) Length   149 a.a.
NCBI ID   WP_010946364.1    Uniprot ID   Q5ZXV4
Organism   Legionella pneumophila strain Pontiac     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 718634..729083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9P85_RS03365 (A9P85_03365) - 718908..719300 (-) 393 WP_010946360.1 hypothetical protein -
  A9P85_RS03370 (A9P85_03370) - 719437..719814 (+) 378 WP_014841112.1 hypothetical protein -
  A9P85_RS03375 (A9P85_03375) - 720016..721323 (+) 1308 WP_014841113.1 tetratricopeptide repeat protein -
  A9P85_RS03380 (A9P85_03380) comEC 721349..723556 (-) 2208 WP_014841114.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  A9P85_RS03385 (A9P85_03385) pilE 723634..724083 (-) 450 WP_010946364.1 type IV pilin protein Machinery gene
  A9P85_RS03390 (A9P85_03390) - 724095..727604 (-) 3510 WP_014841115.1 pilus assembly protein -
  A9P85_RS03395 (A9P85_03395) - 727617..728129 (-) 513 WP_014841116.1 pilus assembly PilX family protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16296.57 Da        Isoelectric Point: 7.6922

>NTDB_id=185473 A9P85_RS03385 WP_010946364.1 723634..724083(-) (pilE) [Legionella pneumophila strain Pontiac]
MLSHVHFMKNSRMKQSAFTLVEVLISMVIMGILVSIAYPSYLQYIQKSRRADAHATLTQDQIILERCYSQNFSYAAACGA
LPAFPQTTPNGYYTINISNLTATTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG

Nucleotide


Download         Length: 450 bp        

>NTDB_id=185473 A9P85_RS03385 WP_010946364.1 723634..724083(-) (pilE) [Legionella pneumophila strain Pontiac]
ATGCTCAGTCATGTACATTTTATGAAGAATAGCCGTATGAAACAATCCGCGTTTACCCTGGTTGAAGTTCTGATCAGCAT
GGTCATTATGGGAATTCTGGTTTCAATTGCCTATCCATCCTATTTACAATATATACAAAAATCCCGTCGTGCTGATGCTC
ATGCCACATTGACACAAGATCAAATCATTTTAGAACGCTGTTATTCACAGAATTTTTCCTATGCGGCAGCGTGTGGCGCC
CTACCAGCATTTCCTCAAACAACGCCGAATGGGTATTATACTATCAATATTTCAAACCTGACAGCCACAACGTATACCTT
AACCGCAACCCCTGTCGGAACCCAAGCCAAAGATACCGAGTGTGCCAGCATGTCAATTAATCAAGCCAATGTAAAAACAG
CCGTAGATTCCTCCGCTAATGCGCAACCAGAATGCTGGAATCCCGGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-38)

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(39-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZXV4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Legionella pneumophila str. Paris

100

100

1
  pilE Legionella pneumophila strain ERS1305867

100

100

1

Multiple sequence alignment