Detailed information NTG0185324

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: A9C19_RS15500
• Genome accession: NZ_CP016020
• Coordinates: 3200042..3203764 (-)
• Length: 1240 a.a.
• NCBI ID: WP_072580778.1
• Uniprot ID: A0A1L3MUG1
• Organism: Bacillus weihaiensis strain Alg07
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 3195042..3208764

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A9C19_RS15490 (A9C19_15215)	-	3195370..3198771 (-)	3402	WP_072580776.1	AAA family ATPase	-
A9C19_RS15495 (A9C19_15220)	-	3198768..3199949 (-)	1182	WP_072580777.1	metallophosphoesterase family protein	-
A9C19_RS15500 (A9C19_15225)	addA	3200042..3203764 (-)	3723	WP_072580778.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
A9C19_RS15505 (A9C19_15230)	addB	3203757..3207248 (-)	3492	WP_072580779.1	helicase-exonuclease AddAB subunit AddB	Machinery gene

Sequence

Protein

• Length: 1240 a.a.
• Molecular weight: 142983.28 Da
• Isoelectric Point: 4.9645

>NTDB_id=185324 A9C19_RS15500 WP_072580778.1 3200042..3203764(-) (addA) [Bacillus weihaiensis strain Alg07]
MSEFMAKPENSQWTDDQWKAIAASGQDILVAAAAGSGKTAVLVERIIRKILSRENPVDVDSLLVVTFTNASAAEMRSRIG
EALEKSLKDNPSSLHLRRQLTLLNKASISTLHSFCLQVVRKYYYLINIDPGFRIADQTEGQLLIDEVLDELFEEEYSDEN
NEEFFDLVDRYTSDRSDMELQTLIRELYFFSRSHPTPTEWLNDLANMYDLKDEADFESLPFLTFLLQDIDMQLSGVREQL
LQAMELTKQPAGPAPRAENLEDDLKQLANIMAHKNSWDQLGEQLKSFKPSRAKIVKGDQYDKKLTDQVTSLRNAAKKQIE
QLRDELFSRSLSNYKQDFLHLKPVVSKLVSLVQQFALRYEGMKKEKGLVDFADLEHYCLQILKGEDDQSSEAALYYKHQF
VEVLVDEYQDTNLVQESILKLVTKDTESAGNLFMVGDVKQSIYRFRLAEPFLFLSKYKRFTQAGDVSGMRIDLSKNFRSR
SEILDGTNYLFKQIMGENVGEIIYDHDAELKLGASYPENEQMSTELLLIEKGGKDNESESEELEQAGLFDEQELETVQLE
ARIMAKRIKKLIEQQFPVYDRGIDGTRPMTYRDVVILLRSMPWAPQIMEEFKQAGIPVYANLSNGYFEATEVAIMMSLLK
VIDNPFQDIPLAAVLRSPIVDATENEMALIRTFDRKGTFYDAVKACVSSEGDERHQSLIQKLKAFVALLESWRDLARQGS
VSELIWQLYRDTKFFDFVGGMPGGKQRQANLRALYDRARQYEATSFRGLFRFLRFIERMQDRGDDLGAARALGEQEDVVR
LMTIHSSKGLEFPVVFVAGLSKQFNMMDLNKKYLLDKELGFGTKLINPKLRVSYPTLPYLALKKKMRMELLAEEMRVLYV
ALTRAKEKLYLVGTLKDAEKKLTDWRNHLSHQEWLLPDFERAKAKSYMDWVGPALIRHKDCYMMSDGMVCLQEEISQHPS
KWTVEKVNGEDLKEVFANEEQINTERLASIQKGEEVSIESDQKALVKEQLTFVYPYLENTKKRSKQSVTELKRQQEVRDE
YSDQQFVKKERSSSFLYNRPNFMQEKSISAAERGTAMHTVMQHLSITEPVTAERVQLKIKELIEKEILTEQLASVIDRHQ
IVQFFSTEVGARMLQAKQVYREVPFSYALKSDGDNEMDEPILVQGVIDCLFEDELGTVLLDYKTDTIKGKFASFIDAEPI
LKDRYQVQIDLYGKAIEEIIQRPLQERYLFFFDGGYLVKM

Nucleotide

• Length: 3723 bp

>NTDB_id=185324 A9C19_RS15500 WP_072580778.1 3200042..3203764(-) (addA) [Bacillus weihaiensis strain Alg07]
ATGAGTGAGTTTATGGCAAAACCAGAAAATAGCCAGTGGACGGATGATCAATGGAAGGCAATCGCTGCTAGTGGACAGGA
TATTTTAGTTGCGGCCGCTGCTGGATCTGGAAAAACAGCTGTATTAGTTGAACGAATTATACGAAAGATTCTTTCAAGGG
AAAACCCTGTAGATGTAGATTCCCTTCTAGTCGTGACATTTACAAATGCATCTGCTGCGGAAATGAGGAGCCGTATTGGA
GAGGCCCTTGAGAAATCACTAAAGGATAACCCATCTTCTCTTCATTTACGTAGACAGCTTACTTTATTAAATAAAGCATC
TATATCAACTCTTCATTCTTTCTGCTTACAGGTTGTAAGGAAGTACTATTATTTAATTAATATTGATCCAGGCTTCCGTA
TTGCTGATCAAACAGAGGGACAACTCCTTATTGATGAGGTACTCGATGAGTTATTTGAAGAAGAATACAGCGATGAAAAT
AATGAAGAGTTTTTTGATTTGGTTGATCGTTATACATCAGACCGAAGTGATATGGAGCTTCAAACACTGATTCGAGAGCT
GTATTTTTTCTCGAGATCTCATCCTACTCCAACCGAGTGGCTTAACGATCTAGCTAACATGTATGATCTTAAAGATGAAG
CGGATTTTGAATCGTTACCATTTTTGACATTCTTACTTCAAGATATCGATATGCAGCTAAGTGGGGTTCGTGAACAATTA
TTACAAGCAATGGAGCTAACAAAGCAGCCAGCAGGTCCTGCCCCACGTGCAGAAAATTTAGAGGATGATTTGAAGCAGCT
TGCCAACATTATGGCGCATAAGAATTCATGGGATCAATTAGGGGAGCAGCTTAAGAGCTTTAAGCCTTCACGTGCAAAAA
TAGTGAAGGGTGATCAATATGATAAAAAGCTGACAGATCAAGTAACATCCTTACGAAATGCAGCAAAAAAGCAAATAGAG
CAATTACGAGATGAATTATTTTCGCGCTCCCTTTCTAATTACAAACAGGATTTTCTACATTTAAAGCCTGTGGTGTCCAA
ACTTGTCTCACTTGTTCAACAATTCGCGCTCCGATACGAGGGAATGAAGAAGGAAAAGGGACTTGTTGATTTTGCTGATT
TAGAGCATTATTGCTTGCAGATCTTAAAGGGTGAAGACGATCAGTCAAGTGAAGCAGCTCTTTATTACAAGCACCAATTT
GTTGAGGTTCTTGTCGATGAATACCAGGATACGAATCTTGTACAAGAATCGATCTTAAAGCTAGTGACAAAGGATACAGA
ATCAGCTGGCAATTTGTTTATGGTTGGTGATGTAAAGCAATCCATTTACCGCTTTCGCTTGGCTGAGCCTTTCCTTTTTC
TGAGTAAATATAAACGATTTACTCAAGCTGGGGACGTATCGGGGATGAGAATAGATTTATCGAAAAACTTCCGAAGTCGT
TCAGAGATTTTAGATGGCACGAACTACTTGTTTAAGCAGATCATGGGCGAGAATGTTGGAGAGATTATCTATGATCATGA
CGCTGAGCTAAAACTAGGTGCAAGCTACCCTGAAAACGAACAAATGAGTACTGAGCTACTCTTAATTGAGAAGGGTGGCA
AGGACAACGAATCTGAGTCCGAGGAGCTAGAGCAAGCTGGTTTATTTGATGAGCAAGAGCTTGAAACGGTCCAGCTAGAA
GCACGAATCATGGCTAAAAGAATAAAAAAACTTATTGAACAACAATTTCCTGTATATGATCGAGGGATAGATGGAACAAG
ACCGATGACGTATCGAGATGTTGTCATACTATTACGTTCTATGCCATGGGCACCTCAAATTATGGAGGAATTTAAACAAG
CGGGCATCCCTGTCTATGCCAATCTTTCTAATGGATATTTCGAAGCAACAGAAGTCGCGATTATGATGTCCTTGCTAAAA
GTCATCGATAATCCATTTCAGGATATTCCTCTAGCAGCTGTGCTACGTTCACCAATCGTAGATGCGACAGAAAACGAAAT
GGCCTTAATTCGTACCTTTGATCGGAAAGGCACGTTTTATGATGCGGTTAAAGCGTGTGTATCCTCGGAAGGTGATGAAA
GGCATCAAAGCTTGATACAGAAGCTAAAAGCATTTGTCGCTTTACTTGAAAGCTGGCGTGACCTTGCTAGACAAGGATCT
GTTTCTGAATTAATTTGGCAGTTATACCGTGATACAAAGTTTTTTGATTTTGTTGGCGGTATGCCGGGTGGAAAGCAGCG
TCAAGCGAATCTTCGTGCCTTATATGATCGAGCAAGACAATACGAAGCAACGTCCTTTAGAGGTTTGTTCCGCTTCCTTC
GTTTTATTGAAAGAATGCAGGATCGTGGCGATGACCTAGGAGCCGCTCGTGCATTAGGTGAACAAGAGGATGTTGTCAGA
CTCATGACAATTCATAGTTCAAAAGGTCTTGAGTTCCCAGTTGTTTTTGTGGCGGGACTTTCTAAGCAATTCAATATGAT
GGATCTAAATAAAAAGTACCTCCTAGATAAAGAATTAGGTTTTGGGACAAAGTTGATTAATCCGAAATTACGTGTGAGCT
ATCCAACGCTTCCATATCTTGCGTTAAAAAAGAAGATGAGGATGGAATTGCTTGCTGAGGAAATGCGGGTCCTTTATGTT
GCATTAACACGTGCGAAGGAAAAGCTTTACTTGGTAGGTACCTTGAAGGATGCAGAAAAGAAGCTTACTGATTGGCGTAA
TCACCTTTCTCATCAAGAGTGGCTTTTACCTGACTTTGAGAGGGCAAAAGCAAAAAGCTATATGGACTGGGTGGGACCAG
CGTTAATCCGCCATAAGGATTGCTATATGATGTCAGATGGAATGGTATGCTTACAGGAGGAGATCTCTCAGCACCCATCT
AAGTGGACTGTTGAAAAGGTAAATGGAGAGGATCTGAAGGAAGTCTTTGCAAATGAAGAGCAAATAAATACGGAGAGATT
AGCTTCCATTCAAAAAGGGGAAGAGGTATCCATTGAAAGTGACCAGAAGGCATTAGTAAAGGAGCAGCTTACGTTTGTTT
ATCCTTATTTAGAAAATACGAAAAAGCGTTCGAAGCAATCTGTAACCGAGTTGAAAAGACAGCAAGAGGTTAGAGATGAA
TACAGCGATCAGCAGTTTGTAAAGAAGGAACGATCGTCATCATTCCTATACAACCGACCTAATTTCATGCAAGAAAAATC
CATTTCAGCAGCTGAACGAGGAACAGCAATGCATACAGTGATGCAACATCTTTCTATTACAGAACCAGTAACAGCTGAGA
GAGTCCAATTGAAAATTAAAGAGCTGATAGAAAAGGAAATCTTAACAGAACAATTAGCGAGTGTGATTGATCGTCATCAA
ATCGTTCAATTCTTTTCAACGGAGGTTGGAGCTAGAATGCTTCAAGCAAAGCAAGTGTATCGTGAGGTTCCATTTAGCTA
TGCGTTGAAATCAGATGGGGATAATGAAATGGATGAGCCAATTTTAGTACAAGGGGTCATCGACTGCTTATTTGAGGATG
AGCTAGGAACGGTTCTATTAGACTATAAAACAGATACGATTAAGGGGAAATTTGCTAGTTTTATTGATGCAGAGCCTATA
TTGAAGGATCGATATCAAGTTCAAATTGATTTATATGGAAAAGCCATTGAAGAAATCATACAAAGACCTCTACAAGAAAG
ATATTTATTTTTCTTTGACGGTGGTTACTTAGTGAAAATGTAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1065-1219)

• UvrD-like helicase C-terminal domain (UvrD_C) (471-892)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (13-460)

3D structure

• AlphaFold DB: A0A1L3MUG1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	58.494	100	0.589