Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EC767_RS04680 Genome accession   NZ_CP020107
Coordinates   930476..930916 (-) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain 13E0767     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 925476..935916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EC767_RS04660 (EC767_04660) coaE 925966..926586 (-) 621 WP_001269531.1 dephospho-CoA kinase -
  EC767_RS26080 - 926611..926655 (+) 45 WP_120795372.1 protein YacM -
  EC767_RS04665 (EC767_04665) guaC 926811..927854 (+) 1044 WP_001217338.1 GMP reductase -
  EC767_RS04670 (EC767_04670) hofC 927889..929091 (-) 1203 WP_000157234.1 protein transport protein HofC -
  EC767_RS04675 (EC767_04675) pilB 929081..930466 (-) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  EC767_RS04680 (EC767_04680) pilA 930476..930916 (-) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  EC767_RS04690 (EC767_04690) nadC 931119..932012 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EC767_RS04695 (EC767_04695) ampD 932100..932651 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EC767_RS04700 (EC767_04700) ampE 932648..933502 (+) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  EC767_RS04705 (EC767_04705) aroP 933545..934918 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=185033 EC767_RS04680 WP_000360902.1 930476..930916(-) (pilA) [Escherichia coli strain 13E0767]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=185033 EC767_RS04680 WP_000360902.1 930476..930916(-) (pilA) [Escherichia coli strain 13E0767]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377