Detailed information NTG0184427

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: A9E89_RS07375
• Genome accession: NZ_CP015947
• Coordinates: 1647602..1648465 (+)
• Length: 287 a.a.
• NCBI ID: WP_015444491.1
• Uniprot ID: I7HPT6
• Organism: Legionella pneumophila strain E2_N
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1642602..1653465

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A9E89_RS07355 (A9E89_07355)	-	1643095..1643421 (-)	327	WP_010947249.1	DUF1820 family protein	-
A9E89_RS07360 (A9E89_07360)	-	1643604..1644347 (-)	744	WP_010947250.1	methyltransferase domain-containing protein	-
A9E89_RS07365 (A9E89_07365)	pilB	1644606..1646330 (+)	1725	WP_010947251.1	type IV-A pilus assembly ATPase PilB	Machinery gene
A9E89_RS07370 (A9E89_07370)	pilC	1646336..1647556 (+)	1221	WP_010947252.1	type II secretion system F family protein	Machinery gene
A9E89_RS07375 (A9E89_07375)	pilD	1647602..1648465 (+)	864	WP_015444491.1	prepilin peptidase	Machinery gene
A9E89_RS07380 (A9E89_07380)	-	1648479..1649684 (-)	1206	WP_015444490.1	cyclic nucleotide-binding domain-containing protein	-
A9E89_RS07385 (A9E89_07385)	-	1649792..1650385 (-)	594	WP_010947255.1	hypothetical protein	-
A9E89_RS07390 (A9E89_07390)	-	1650401..1650856 (-)	456	WP_010947256.1	hypothetical protein	-
A9E89_RS07395 (A9E89_07395)	ppsR	1650990..1651805 (+)	816	WP_010947257.1	posphoenolpyruvate synthetase regulatory kinase/phosphorylase PpsR	-
A9E89_RS07400 (A9E89_07400)	-	1651930..1653378 (-)	1449	WP_015444489.1	bifunctional 2-methylcitrate dehydratase/aconitate hydratase	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 32769.36 Da
• Isoelectric Point: 7.8442

>NTDB_id=184427 A9E89_RS07375 WP_015444491.1 1647602..1648465(+) (pilD) [Legionella pneumophila strain E2_N]
MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHLEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNVFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM

Nucleotide

• Length: 864 bp

>NTDB_id=184427 A9E89_RS07375 WP_015444491.1 1647602..1648465(+) (pilD) [Legionella pneumophila strain E2_N]
ATGATAAATGCACTAATAATCAATTATCCATGGTTTATGTATTTAGTCGTTGGACTCTTTTCTCTAGCCGTTGGAAGTTT
GCTTAATGTGATAATCTATCGCTTACCAATTATTTTGCAGGAAGAGTGGAAAGAGCAATGCTGTGAGCTATTCCATCTTG
AACAGCGAAAAGAAAAAATAAAACTTAATTTATTTTTACCACGTTCTTTTTGTCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCTCTGTTAAGCTATATTTTGTTAAGAGGACGCTGTTACCAATGTGATAGTCCAATTTCAATCCGCTA
CCCTTTTGTTGAGACGTTGACCACAGTTTTATCCTTATACGCCAGTTGGCATTTTGGGTTTACAATACAATTATTATTTG
CTTTATTAGCTATCTGGATATTAATATCACTTGTTTTTATTGATCTTGATCATCAATTACTCCCTGATAGCCTCACTTTA
GGGCTCTTATGGATTGGCCTTATTGCTAATACCCAGAATGTATTTGTTAGTCTTGACGTTGCTGTATTAAGCTGCGCTGG
CGCTTATTTAGCCTTATGGCTATTCATTAATCTGTTTTATCTGATGACAGGTAAAGTAGGTATGGGACATGGAGATTTCA
AATTATTTGCAGCATTTGGTGCCTGGCTTGGATGGATGTATTTACCATTAATTCTACTAATATCTTCAATAACGGGGGCT
ATCATTGGTTTGATTTATTTAAAGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGCCCTTTTCTTTGCATTTC
TGGATTAATAGCTATGTTTTGGGGAGACTCAATCATCAATTGGTATATAGGTTATTGGATGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

• Type IV leader peptidase family (Peptidase_A24) (136-244)

3D structure

• AlphaFold DB: I7HPT6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.11	97.909	0.481
pilD	Acinetobacter nosocomialis M2	47.985	95.122	0.456
pilD	Acinetobacter baumannii D1279779	47.273	95.819	0.453
pilD	Vibrio campbellii strain DS40M4	45.324	96.864	0.439
pilD	Neisseria gonorrhoeae MS11	42.806	96.864	0.415