Detailed information NTG0182827

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: LL158_RS06240
• Genome accession: NZ_CP015894
• Coordinates: 1230307..1231155 (+)
• Length: 282 a.a.
• NCBI ID: WP_015082464.1
• Uniprot ID: A0AAJ6N2R3
• Organism: Lactococcus cremoris strain 158
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1225307..1236155

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL158_RS06215 (LL158_1194)	ilvN	1226040..1226516 (+)	477	WP_015082461.1	acetolactate synthase small subunit	-
LL158_RS06220 (LL158_1195)	ilvC	1226562..1227584 (+)	1023	WP_015082462.1	ketol-acid reductoisomerase	-
LL158_RS06225 (LL158_1196)	ilvA	1227643..1228893 (+)	1251	WP_014572605.1	threonine ammonia-lyase IlvA	-
LL158_RS06230 (LL158_1197)	budA	1229028..1229738 (+)	711	WP_011676302.1	acetolactate decarboxylase	-
LL158_RS06235 (LL158_1198)	-	1229778..1230158 (+)	381	WP_015082463.1	RidA family protein	-
LL158_RS06240 (LL158_1199)	dprA	1230307..1231155 (+)	849	WP_015082464.1	DNA-processing protein DprA	Machinery gene
LL158_RS06245 (LL158_1200)	topA	1231421..1233547 (+)	2127	WP_015082465.1	type I DNA topoisomerase	-
LL158_RS06250 (LL158_1201)	trmFO	1233619..1234965 (+)	1347	WP_014572600.1	methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO	-
LL158_RS06255 (LL158_1202)	xerS	1235015..1236085 (-)	1071	WP_015082466.1	tyrosine recombinase XerS	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31854.03 Da
• Isoelectric Point: 9.8386

>NTDB_id=182827 LL158_RS06240 WP_015082464.1 1230307..1231155(+) (dprA) [Lactococcus cremoris strain 158]
MITNFDLFRWKKAGMTNLGVNKLLKFFLKYDIKISLRQMGQVAQVKSIPNFIEQYKNQDVKKLRIDYKKFSSFSILDDLY
PERLREIYNPPVLIFYQGNIDLLKNPKLAFVGSRLAGQSGIKAVQKIITELNQSFTIVSGLAKGIDTASHLSAIKTKTPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLVLSEYSLGEKPLKYHFPERNRIIAGLSRGVVVVEAKLRSGSLITCERALEEG
RDIFAIPGNIADGTSDGCNHLIQQGAKLVYQAQDILEEYLYN

Nucleotide

• Length: 849 bp

>NTDB_id=182827 LL158_RS06240 WP_015082464.1 1230307..1231155(+) (dprA) [Lactococcus cremoris strain 158]
ATGATTACAAATTTTGATTTATTTCGTTGGAAAAAAGCAGGAATGACCAATCTTGGTGTAAATAAGCTCTTAAAATTTTT
CCTCAAATATGATATAAAAATAAGCTTACGACAAATGGGACAAGTGGCTCAAGTCAAATCAATCCCTAACTTTATCGAAC
AATATAAAAATCAGGACGTAAAGAAACTCAGGATAGACTATAAAAAATTTTCCTCCTTTTCCATTCTTGATGACCTTTAT
CCCGAGCGTCTAAGAGAAATCTACAACCCACCCGTTTTAATTTTTTATCAGGGAAATATTGATTTATTAAAAAATCCTAA
ACTTGCCTTTGTTGGAAGCCGCCTAGCAGGTCAATCAGGGATAAAAGCTGTCCAAAAAATTATCACAGAATTGAATCAAT
CCTTTACAATAGTAAGCGGGCTTGCTAAAGGGATTGACACAGCCAGCCATCTGTCAGCAATTAAAACTAAAACACCAACA
ATTGCTGTTATTGGTACAGGATTGGATATTTTCTATCCACTAGAAAATCGTAAAATACAGGAATATCTAGCAAAAAATCA
ATTAGTTTTAAGTGAATATTCATTAGGCGAAAAACCACTTAAATACCACTTTCCAGAGCGCAATCGAATTATTGCTGGTC
TTTCACGGGGAGTTGTTGTCGTGGAAGCAAAATTAAGAAGTGGCAGTTTAATCACCTGTGAAAGGGCACTGGAAGAAGGA
AGAGATATCTTTGCCATCCCTGGAAATATTGCAGATGGAACATCTGATGGCTGTAATCATCTTATTCAACAAGGAGCAAA
ACTTGTCTATCAAGCTCAAGATATTTTAGAAGAATATTTGTACAATTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (69-270)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (2-63)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Lactococcus lactis subsp. cremoris KW2	97.872	100	0.979
dprA	Streptococcus mutans UA159	59.353	98.582	0.585
dprA/cilB/dalA	Streptococcus mitis SK321	56.071	99.291	0.557
dprA/cilB/dalA	Streptococcus pneumoniae R6	55.714	99.291	0.553
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	55.714	99.291	0.553
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	55.714	99.291	0.553
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	55.714	99.291	0.553
dprA/cilB/dalA	Streptococcus pneumoniae D39	55.714	99.291	0.553
dprA	Vibrio campbellii strain DS40M4	35.644	100	0.383
dprA	Bacillus subtilis subsp. subtilis str. 168	39.273	97.518	0.383
dprA	Legionella pneumophila strain ERS1305867	39.13	97.872	0.383
dprA	Haemophilus influenzae Rd KW20	39.63	95.745	0.379