Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   AsACE_RS12925 Genome accession   NZ_CP015615
Coordinates   2661919..2662434 (-) Length   171 a.a.
NCBI ID   WP_076754944.1    Uniprot ID   -
Organism   Acinetobacter schindleri strain ACE     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2658110..2679518 2661919..2662434 within 0


Gene organization within MGE regions


Location: 2658110..2679518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AsACE_RS12890 - 2658110..2658307 (+) 198 WP_076754455.1 hypothetical protein -
  AsACE_RS16370 - 2658336..2658791 (+) 456 WP_167367456.1 esterase/lipase family protein -
  AsACE_RS16375 - 2658867..2659085 (+) 219 WP_167367457.1 hypothetical protein -
  AsACE_RS12900 (AsACE_CH02568) rplS 2659152..2659523 (-) 372 WP_004787066.1 50S ribosomal protein L19 -
  AsACE_RS12905 (AsACE_CH02569) trmD 2659698..2660447 (-) 750 WP_076754457.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  AsACE_RS12910 (AsACE_CH02570) rimM 2660490..2661038 (-) 549 WP_076754459.1 ribosome maturation factor RimM -
  AsACE_RS12915 (AsACE_CH02571) rpsP 2661057..2661314 (-) 258 WP_076754461.1 30S ribosomal protein S16 -
  AsACE_RS12920 (AsACE_CH02572) - 2661458..2661925 (-) 468 WP_048881779.1 type IV pilin protein -
  AsACE_RS12925 (AsACE_CH02573) comE 2661919..2662434 (-) 516 WP_076754944.1 type IV pilin protein Machinery gene
  AsACE_RS12930 (AsACE_CH02574) - 2662445..2666509 (-) 4065 WP_076754462.1 PilC/PilY family type IV pilus protein -
  AsACE_RS12935 (AsACE_CH02575) - 2666520..2667266 (-) 747 WP_048881777.1 PilX N-terminal domain-containing pilus assembly protein -
  AsACE_RS12940 (AsACE_CH02576) - 2667263..2668216 (-) 954 WP_053215401.1 PilW family protein -
  AsACE_RS12945 (AsACE_CH02577) pilV 2668219..2668713 (-) 495 WP_048881848.1 type IV pilus modification protein PilV -
  AsACE_RS12950 (AsACE_CH02578) - 2668713..2669225 (-) 513 WP_071319809.1 Tfp pilus assembly protein FimT/FimU -
  AsACE_RS12955 (AsACE_CH02579) ispH 2669385..2670335 (-) 951 WP_076754464.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  AsACE_RS12960 (AsACE_CH02580) gmk 2670470..2671093 (+) 624 WP_004896246.1 guanylate kinase -
  AsACE_RS12965 (AsACE_CH02581) rpoZ 2671168..2671449 (+) 282 WP_004896248.1 DNA-directed RNA polymerase subunit omega -
  AsACE_RS12970 (AsACE_CH02582) - 2671667..2673772 (+) 2106 WP_076754466.1 RelA/SpoT family protein -
  AsACE_RS12975 (AsACE_CH02583) - 2673848..2674231 (+) 384 WP_005223441.1 RidA family protein -
  AsACE_RS12980 (AsACE_CH02584) - 2674452..2674646 (+) 195 WP_076754467.1 bacterioferritin-associated ferredoxin -
  AsACE_RS12985 (AsACE_CH02585) bfr 2674888..2675352 (+) 465 WP_076754470.1 bacterioferritin -
  AsACE_RS12990 (AsACE_CH02586) - 2675408..2677045 (-) 1638 WP_076754472.1 PglL family O-oligosaccharyltransferase -
  AsACE_RS12995 (AsACE_CH02587) tfpZ 2677132..2677854 (-) 723 WP_076754474.1 TfpX/TfpZ family type IV pilin accessory protein -
  AsACE_RS13000 (AsACE_CH02588) - 2677851..2679518 (-) 1668 WP_076754476.1 PglL family O-oligosaccharyltransferase -

Sequence

Protein


Download         Length: 171 a.a.        Molecular weight: 18694.25 Da        Isoelectric Point: 9.7876

>NTDB_id=181533 AsACE_RS12925 WP_076754944.1 2661919..2662434(-) (comE) [Acinetobacter schindleri strain ACE]
MRIPQRGFTLIELMVVVAIVAILATIAIPSYQTHTRKAIAGQAQQEIQKLAEQLERHKGKNFSYLGFDPGYLYKDNSGNL
IGYSTTNAQLNLPLNSSGTAVKYIVSVRDGTDTTKLLTNTTALGQRWAIKAESQDPNNFTFLMTSTGIQCKNQTRANVSY
TGCGTGGVNTW

Nucleotide


Download         Length: 516 bp        

>NTDB_id=181533 AsACE_RS12925 WP_076754944.1 2661919..2662434(-) (comE) [Acinetobacter schindleri strain ACE]
ATGCGTATACCGCAACGAGGATTTACATTAATTGAATTAATGGTGGTGGTTGCAATTGTGGCTATTCTTGCCACCATTGC
AATCCCGAGTTATCAGACTCATACCCGAAAGGCAATCGCTGGTCAAGCCCAGCAGGAAATACAAAAGCTAGCAGAGCAGT
TGGAGCGTCATAAAGGTAAAAATTTCAGCTATTTAGGTTTTGATCCTGGCTATTTGTATAAAGATAATAGTGGAAATTTA
ATTGGTTATAGTACAACTAATGCGCAGCTAAACCTGCCATTAAATAGTTCAGGAACCGCAGTTAAATATATTGTTTCTGT
TCGAGATGGTACAGATACTACTAAACTTTTAACTAATACTACCGCTTTAGGACAAAGATGGGCTATTAAAGCAGAAAGCC
AAGATCCGAATAATTTTACCTTTCTTATGACGAGTACAGGAATCCAGTGTAAAAATCAGACTAGAGCAAATGTATCTTAT
ACAGGGTGTGGCACAGGAGGAGTGAATACGTGGTAA

Domains

Predicted by InterproScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-164)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Acinetobacter baylyi ADP1

54.762

98.246

0.538
  pilY2 Acinetobacter baumannii D1279779

44.512

95.906

0.427

Multiple sequence alignment