Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   BSA171_RS08225 Genome accession   NZ_CP015611
Coordinates   1588119..1588412 (+) Length   97 a.a.
NCBI ID   WP_024422977.1    Uniprot ID   A0A0M2EIN6
Organism   Bacillus safensis strain U17-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1583119..1593412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSA171_RS08190 (BSA171_08070) - 1583210..1583848 (+) 639 WP_039179959.1 MBL fold metallo-hydrolase -
  BSA171_RS08195 (BSA171_08075) - 1583896..1584138 (-) 243 WP_003217440.1 DUF2626 domain-containing protein -
  BSA171_RS08200 (BSA171_08080) - 1584353..1584733 (+) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BSA171_RS08210 (BSA171_08090) - 1584958..1585833 (+) 876 WP_039179956.1 STAS domain-containing protein -
  BSA171_RS08215 (BSA171_08095) comGA 1586011..1587081 (+) 1071 WP_081378155.1 competence type IV pilus ATPase ComGA Machinery gene
  BSA171_RS08220 (BSA171_08100) comGB 1587023..1588102 (+) 1080 WP_231120189.1 competence type IV pilus assembly protein ComGB -
  BSA171_RS08225 (BSA171_08105) comGC 1588119..1588412 (+) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  BSA171_RS08230 (BSA171_08110) comGD 1588405..1588851 (+) 447 WP_075611640.1 competence type IV pilus minor pilin ComGD -
  BSA171_RS08235 (BSA171_08115) comGE 1588835..1589152 (+) 318 WP_075611639.1 competence type IV pilus minor pilin ComGE -
  BSA171_RS08240 (BSA171_08120) comGF 1589061..1589591 (+) 531 WP_196770529.1 competence type IV pilus minor pilin ComGF -
  BSA171_RS08245 (BSA171_08125) - 1589588..1589971 (+) 384 WP_075611637.1 competence type IV pilus minor pilin ComGG -
  BSA171_RS08250 (BSA171_08130) - 1590031..1590225 (+) 195 WP_024718846.1 YqzE family protein -
  BSA171_RS08255 (BSA171_08135) - 1590258..1590536 (-) 279 WP_231120214.1 DUF3889 domain-containing protein -
  BSA171_RS08260 (BSA171_08140) tapA 1590836..1591363 (+) 528 WP_075611636.1 amyloid fiber anchoring/assembly protein TapA -
  BSA171_RS08265 (BSA171_08145) sipW 1591415..1591987 (+) 573 WP_047202287.1 signal peptidase I SipW -
  BSA171_RS08270 (BSA171_08150) tasA 1592045..1592845 (+) 801 WP_075611635.1 biofilm matrix protein TasA -
  BSA171_RS08275 (BSA171_08155) sinR 1592913..1593248 (-) 336 WP_024422969.1 transcriptional regulator SinR Regulator

Sequence


Protein


Download         Length: 97 a.a.        Molecular weight: 10686.62 Da        Isoelectric Point: 7.3218

>NTDB_id=181446 BSA171_RS08225 WP_024422977.1 1588119..1588412(+) (comGC) [Bacillus safensis strain U17-1]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQAIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGNVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=181446 BSA171_RS08225 WP_024422977.1 1588119..1588412(+) (comGC) [Bacillus safensis strain U17-1]
ATGAATGATAAAGGGTTTACCCTGTTAGAAATGTTAATTGTCATGCTCGTGATATCCATTCTTTTACTCATTACCATACC
GAATATCACACGTCATCACCAAGCAATTCAAGCAAAGGGCTGTGAAGGGCTCAAATCAATGGTCAGCACACAAGTGATGG
CATATGAATTGGAGCATGATGGGAAGACACCATCTTTAGCTGAGTTAGAAAAAGAAGGGTATGTTAAAAAGGGATTAACC
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCAACGTGAAGCATGAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0M2EIN6

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67

  comGC Staphylococcus aureus MW2

42.222

92.784

0.392

  comGC Staphylococcus aureus N315

42.222

92.784

0.392


Multiple sequence alignment