Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BSA171_RS08215 Genome accession   NZ_CP015611
Coordinates   1586011..1587081 (+) Length   356 a.a.
NCBI ID   WP_081378155.1    Uniprot ID   -
Organism   Bacillus safensis strain U17-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1581011..1592081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSA171_RS08175 (BSA171_08060) - 1581210..1582463 (+) 1254 WP_075611642.1 MFS transporter -
  BSA171_RS08180 (BSA171_08065) - 1582540..1582854 (+) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  BSA171_RS08185 - 1582879..1583052 (-) 174 WP_003217333.1 DUF2759 domain-containing protein -
  BSA171_RS08190 (BSA171_08070) - 1583210..1583848 (+) 639 WP_039179959.1 MBL fold metallo-hydrolase -
  BSA171_RS08195 (BSA171_08075) - 1583896..1584138 (-) 243 WP_003217440.1 DUF2626 domain-containing protein -
  BSA171_RS08200 (BSA171_08080) - 1584353..1584733 (+) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BSA171_RS08210 (BSA171_08090) - 1584958..1585833 (+) 876 WP_039179956.1 STAS domain-containing protein -
  BSA171_RS08215 (BSA171_08095) comGA 1586011..1587081 (+) 1071 WP_081378155.1 competence type IV pilus ATPase ComGA Machinery gene
  BSA171_RS08220 (BSA171_08100) comGB 1587023..1588102 (+) 1080 WP_231120189.1 competence type IV pilus assembly protein ComGB -
  BSA171_RS08225 (BSA171_08105) comGC 1588119..1588412 (+) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  BSA171_RS08230 (BSA171_08110) comGD 1588405..1588851 (+) 447 WP_075611640.1 competence type IV pilus minor pilin ComGD -
  BSA171_RS08235 (BSA171_08115) comGE 1588835..1589152 (+) 318 WP_075611639.1 competence type IV pilus minor pilin ComGE -
  BSA171_RS08240 (BSA171_08120) comGF 1589061..1589591 (+) 531 WP_196770529.1 competence type IV pilus minor pilin ComGF -
  BSA171_RS08245 (BSA171_08125) - 1589588..1589971 (+) 384 WP_075611637.1 competence type IV pilus minor pilin ComGG -
  BSA171_RS08250 (BSA171_08130) - 1590031..1590225 (+) 195 WP_024718846.1 YqzE family protein -
  BSA171_RS08255 (BSA171_08135) - 1590258..1590536 (-) 279 WP_231120214.1 DUF3889 domain-containing protein -
  BSA171_RS08260 (BSA171_08140) tapA 1590836..1591363 (+) 528 WP_075611636.1 amyloid fiber anchoring/assembly protein TapA -
  BSA171_RS08265 (BSA171_08145) sipW 1591415..1591987 (+) 573 WP_047202287.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40418.92 Da        Isoelectric Point: 9.4005

>NTDB_id=181445 BSA171_RS08215 WP_081378155.1 1586011..1587081(+) (comGA) [Bacillus safensis strain U17-1]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEALVSFRVDSDLIQQRTIDKKTGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRSGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLKHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYASGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRSIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLPKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=181445 BSA171_RS08215 WP_081378155.1 1586011..1587081(+) (comGA) [Bacillus safensis strain U17-1]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCACATTGTCCC
AAAAGAAAAGGAAGCGTTGGTATCTTTCCGCGTAGACTCAGATTTAATCCAGCAGCGAACCATTGATAAAAAAACCGGTG
AGCGGCTCATTGCTCATTTTAAATTTTTATCATCCATGGATATCGGAGAAAAACGGAGACCGCAAAATGGGTCATTAGCT
GTGATGCTAAGAAGCGGCCAGGTGTTTATTCGAATGTCTACCTTACCAACTGTAAATGATGAGAGTTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCGAAAATAAAACATCTGTCACTATTTCCGAAAGCTTCAGCCAGGTTATTGTCATTTTTGA
AGCATTCGCATGGGCTCATTTTATTTACCGGTCCAACCAATTCGGGGAAAACAACAACCCTCTATTCACTGATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGTTACAGGTTCA
AGTAAATGAAAAAGCCGGCATCACATATGCCTCAGGATTACGCGCTATCTTAAGACATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCCCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGTCAGCTTTACTGTAAATTAAATCGAT
CAATCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTTGGCTATCTACCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.972

99.438

0.646

Multiple sequence alignment