Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BSA41_RS09855 Genome accession   NZ_CP015610
Coordinates   1870561..1871631 (-) Length   356 a.a.
NCBI ID   WP_081378155.1    Uniprot ID   -
Organism   Bacillus safensis strain U41     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1865561..1876631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSA41_RS09805 (BSA41_09575) sipW 1865655..1866227 (-) 573 WP_047202287.1 signal peptidase I SipW -
  BSA41_RS09810 (BSA41_09580) tapA 1866279..1866806 (-) 528 WP_075611636.1 amyloid fiber anchoring/assembly protein TapA -
  BSA41_RS09815 (BSA41_09585) - 1867106..1867384 (+) 279 WP_231120214.1 DUF3889 domain-containing protein -
  BSA41_RS09820 (BSA41_09590) - 1867417..1867611 (-) 195 WP_024718846.1 YqzE family protein -
  BSA41_RS09825 (BSA41_09595) - 1867671..1868054 (-) 384 WP_075611637.1 competence type IV pilus minor pilin ComGG -
  BSA41_RS09830 (BSA41_09600) comGF 1868051..1868455 (-) 405 WP_231120191.1 competence type IV pilus minor pilin ComGF -
  BSA41_RS09835 (BSA41_09605) comGE 1868490..1868807 (-) 318 WP_075611639.1 competence type IV pilus minor pilin ComGE -
  BSA41_RS09840 (BSA41_09610) comGD 1868791..1869237 (-) 447 WP_075611640.1 competence type IV pilus minor pilin ComGD -
  BSA41_RS09845 (BSA41_09615) comGC 1869230..1869523 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  BSA41_RS09850 (BSA41_09620) comGB 1869540..1870619 (-) 1080 WP_231120189.1 competence type IV pilus assembly protein ComGB -
  BSA41_RS09855 (BSA41_09625) comGA 1870561..1871631 (-) 1071 WP_081378155.1 competence type IV pilus ATPase ComGA Machinery gene
  BSA41_RS09860 (BSA41_09630) - 1871809..1872684 (-) 876 WP_039179956.1 STAS domain-containing protein -
  BSA41_RS09870 (BSA41_09640) - 1872909..1873289 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BSA41_RS09875 (BSA41_09645) - 1873504..1873746 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  BSA41_RS09880 (BSA41_09650) - 1873794..1874432 (-) 639 WP_039179959.1 MBL fold metallo-hydrolase -
  BSA41_RS09885 - 1874590..1874763 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  BSA41_RS09890 (BSA41_09655) - 1874788..1875102 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  BSA41_RS09895 (BSA41_09660) - 1875179..1876432 (-) 1254 WP_075611642.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40418.92 Da        Isoelectric Point: 9.4005

>NTDB_id=181388 BSA41_RS09855 WP_081378155.1 1870561..1871631(-) (comGA) [Bacillus safensis strain U41]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEALVSFRVDSDLIQQRTIDKKTGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRSGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLKHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYASGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRSIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLPKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=181388 BSA41_RS09855 WP_081378155.1 1870561..1871631(-) (comGA) [Bacillus safensis strain U41]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCACATTGTCCC
AAAAGAAAAGGAAGCGTTGGTATCTTTCCGCGTAGACTCAGATTTAATCCAGCAGCGAACCATTGATAAAAAAACCGGTG
AGCGGCTCATTGCTCATTTTAAATTTTTATCATCCATGGATATCGGAGAAAAACGGAGACCGCAAAATGGGTCATTAGCT
GTGATGCTAAGAAGCGGCCAGGTGTTTATTCGAATGTCTACCTTACCAACTGTAAATGATGAGAGTTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCGAAAATAAAACATCTGTCACTATTTCCGAAAGCTTCAGCCAGGTTATTGTCATTTTTGA
AGCATTCGCATGGGCTCATTTTATTTACCGGTCCAACCAATTCGGGGAAAACAACAACCCTCTATTCACTGATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGTTACAGGTTCA
AGTAAATGAAAAAGCCGGCATCACATATGCCTCAGGATTACGCGCTATCTTAAGACATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCCCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGTCAGCTTTACTGTAAATTAAATCGAT
CAATCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTTGGCTATCTACCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.972

99.438

0.646

Multiple sequence alignment