Detailed information NTG0178584

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: A5479_RS03145
• Genome accession: NZ_CP015344
• Coordinates: 670092..670541 (-)
• Length: 149 a.a.
• NCBI ID: WP_010946364.1
• Uniprot ID: Q5ZXV4
• Organism: Legionella pneumophila strain D-7630
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 665092..675541

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A5479_RS03125 (A5479_03125)	-	665337..665729 (-)	393	WP_011945786.1	hypothetical protein	-
A5479_RS03130 (A5479_03130)	-	665866..666243 (+)	378	WP_011945787.1	hypothetical protein	-
A5479_RS03135 (A5479_03135)	-	666444..667751 (+)	1308	WP_011945788.1	tetratricopeptide repeat protein	-
A5479_RS03140 (A5479_03140)	comEC	667807..670014 (-)	2208	WP_011945789.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
A5479_RS03145 (A5479_03145)	pilE	670092..670541 (-)	450	WP_010946364.1	type IV pilin protein	Machinery gene
A5479_RS03150 (A5479_03150)	-	670553..674062 (-)	3510	WP_011945790.1	pilus assembly protein	-
A5479_RS03155 (A5479_03155)	-	674075..674587 (-)	513	WP_011945791.1	pilus assembly PilX family protein	-

Sequence

Protein

• Length: 149 a.a.
• Molecular weight: 16296.57 Da
• Isoelectric Point: 7.6922

>NTDB_id=178584 A5479_RS03145 WP_010946364.1 670092..670541(-) (pilE) [Legionella pneumophila strain D-7630]
MLSHVHFMKNSRMKQSAFTLVEVLISMVIMGILVSIAYPSYLQYIQKSRRADAHATLTQDQIILERCYSQNFSYAAACGA
LPAFPQTTPNGYYTINISNLTATTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG

Nucleotide

• Length: 450 bp

>NTDB_id=178584 A5479_RS03145 WP_010946364.1 670092..670541(-) (pilE) [Legionella pneumophila strain D-7630]
ATGCTCAGTCATGTACATTTTATGAAGAATAGCCGTATGAAACAATCCGCGTTTACCCTGGTTGAAGTTCTGATCAGCAT
GGTCATTATGGGAATTCTGGTTTCAATTGCCTATCCATCCTATTTACAATATATACAAAAATCCCGTCGTGCTGATGCTC
ATGCCACATTGACACAAGATCAAATCATTTTAGAACGCTGTTATTCACAGAATTTTTCCTATGCGGCAGCGTGTGGCGCC
CTACCAGCATTTCCTCAAACAACGCCGAATGGGTATTATACTATCAATATTTCAAACCTGACAGCCACAACGTATACCTT
AACCGCAACCCCTGTCGGAACCCAAGCCAAAGATACCGAGTGTGCCAGTATGTCAATTAACCAGGCCAATGTAAAAACAG
CCGTAGATTCCTCCGCTAATGCGCAACCAGAATGCTGGAATCCCGGTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (12-38)

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (39-124)

3D structure

• AlphaFold DB: Q5ZXV4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Legionella pneumophila str. Paris	100	100	1
pilE	Legionella pneumophila strain ERS1305867	100	100	1