Detailed information NTG0178542

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: A5480_RS03145
• Genome accession: NZ_CP015343
• Coordinates: 670091..670540 (-)
• Length: 149 a.a.
• NCBI ID: WP_010946364.1
• Uniprot ID: Q5ZXV4
• Organism: Legionella pneumophila strain D-7631
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 665091..675540

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A5480_RS03125 (A5480_03125)	-	665336..665728 (-)	393	WP_011945786.1	hypothetical protein	-
A5480_RS03130 (A5480_03130)	-	665865..666242 (+)	378	WP_011945787.1	hypothetical protein	-
A5480_RS03135 (A5480_03135)	-	666443..667750 (+)	1308	WP_011945788.1	tetratricopeptide repeat protein	-
A5480_RS03140 (A5480_03140)	comEC	667806..670013 (-)	2208	WP_011945789.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
A5480_RS03145 (A5480_03145)	pilE	670091..670540 (-)	450	WP_010946364.1	type IV pilin protein	Machinery gene
A5480_RS03150 (A5480_03150)	-	670552..674061 (-)	3510	WP_011945790.1	pilus assembly protein	-
A5480_RS03155 (A5480_03155)	-	674074..674586 (-)	513	WP_011945791.1	pilus assembly PilX family protein	-

Sequence

Protein

• Length: 149 a.a.
• Molecular weight: 16296.57 Da
• Isoelectric Point: 7.6922

>NTDB_id=178542 A5480_RS03145 WP_010946364.1 670091..670540(-) (pilE) [Legionella pneumophila strain D-7631]
MLSHVHFMKNSRMKQSAFTLVEVLISMVIMGILVSIAYPSYLQYIQKSRRADAHATLTQDQIILERCYSQNFSYAAACGA
LPAFPQTTPNGYYTINISNLTATTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG

Nucleotide

• Length: 450 bp

>NTDB_id=178542 A5480_RS03145 WP_010946364.1 670091..670540(-) (pilE) [Legionella pneumophila strain D-7631]
ATGCTCAGTCATGTACATTTTATGAAGAATAGCCGTATGAAACAATCCGCGTTTACCCTGGTTGAAGTTCTGATCAGCAT
GGTCATTATGGGAATTCTGGTTTCAATTGCCTATCCATCCTATTTACAATATATACAAAAATCCCGTCGTGCTGATGCTC
ATGCCACATTGACACAAGATCAAATCATTTTAGAACGCTGTTATTCACAGAATTTTTCCTATGCGGCAGCGTGTGGCGCC
CTACCAGCATTTCCTCAAACAACGCCGAATGGGTATTATACTATCAATATTTCAAACCTGACAGCCACAACGTATACCTT
AACCGCAACCCCTGTCGGAACCCAAGCCAAAGATACCGAGTGTGCCAGTATGTCAATTAACCAGGCCAATGTAAAAACAG
CCGTAGATTCCTCCGCTAATGCGCAACCAGAATGCTGGAATCCCGGTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (12-38)

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (39-124)

3D structure

• AlphaFold DB: Q5ZXV4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Legionella pneumophila str. Paris	100	100	1
pilE	Legionella pneumophila strain ERS1305867	100	100	1