Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BTI679_RS22205 Genome accession   NZ_CP015257
Coordinates   4262935..4263978 (-) Length   347 a.a.
NCBI ID   WP_064474804.1    Uniprot ID   A0A1A9PWS3
Organism   Bacillus wiedmannii strain Bt18679     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4257935..4268978
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTI679_RS22160 (BTI679_44960) - 4258364..4258564 (-) 201 WP_000106080.1 YqzE family protein -
  BTI679_RS22165 (BTI679_44970) - 4258603..4259100 (-) 498 WP_098111410.1 shikimate kinase -
  BTI679_RS22170 (BTI679_44980) - 4259219..4259869 (-) 651 WP_064474809.1 2OG-Fe(II) oxygenase -
  BTI679_RS22175 (BTI679_44990) comGG 4260045..4260416 (-) 372 WP_046956345.1 competence type IV pilus minor pilin ComGG -
  BTI679_RS22180 (BTI679_45000) comGF 4260413..4260883 (-) 471 WP_064474808.1 competence type IV pilus minor pilin ComGF -
  BTI679_RS22185 (BTI679_45010) comGE 4260853..4261155 (-) 303 WP_064474807.1 competence type IV pilus minor pilin ComGE -
  BTI679_RS22190 (BTI679_45020) comGD 4261148..4261603 (-) 456 WP_098111411.1 comG operon protein ComGD -
  BTI679_RS22195 (BTI679_45030) comGC 4261600..4261899 (-) 300 WP_001178693.1 competence type IV pilus major pilin ComGC -
  BTI679_RS22200 (BTI679_45040) comGB 4261911..4262942 (-) 1032 WP_088048114.1 competence type IV pilus assembly protein ComGB -
  BTI679_RS22205 (BTI679_45050) comGA 4262935..4263978 (-) 1044 WP_064474804.1 competence type IV pilus ATPase ComGA Machinery gene
  BTI679_RS22210 (BTI679_45060) - 4264183..4264878 (+) 696 WP_000434122.1 helix-turn-helix domain-containing protein -
  BTI679_RS22215 (BTI679_45070) - 4265004..4265246 (+) 243 WP_000440714.1 DUF2626 domain-containing protein -
  BTI679_RS22220 (BTI679_45080) - 4265353..4266672 (+) 1320 WP_103655766.1 cation:dicarboxylase symporter family transporter -
  BTI679_RS22225 (BTI679_45090) - 4266880..4267287 (-) 408 WP_064474803.1 hypothetical protein -
  BTI679_RS22230 (BTI679_45100) - 4267386..4267601 (-) 216 WP_064474802.1 DUF3912 family protein -
  BTI679_RS22235 (BTI679_45110) - 4267856..4268167 (+) 312 WP_001093232.1 hypothetical protein -
  BTI679_RS22240 (BTI679_45120) - 4268208..4268696 (-) 489 WP_142314999.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39170.65 Da        Isoelectric Point: 8.8958

>NTDB_id=178131 BTI679_RS22205 WP_064474804.1 4262935..4263978(-) (comGA) [Bacillus wiedmannii strain Bt18679]
MNGIEIFANMILKEACRVQASDLHIVPRKKDVAVQLRIGKDLMTKHCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKILSFLHYSHGLLVFTGPTGSGKTTTMYALLEA
IRKKKTRRIITLEDPVEKRNDDVLQIQINEKAGITYETGLKAILRHDPDIILVGEIRDEETAKIAVRASLTGHLVMTTLH
TSDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGNCSVLCKSMRQVRQASIYELLYGYELKQAIKGANGEC
VTYKHETLESSLRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=178131 BTI679_RS22205 WP_064474804.1 4262935..4263978(-) (comGA) [Bacillus wiedmannii strain Bt18679]
ATGAATGGAATTGAAATTTTTGCGAATATGATTTTGAAAGAGGCGTGCAGGGTACAAGCTTCGGATTTACATATTGTGCC
CCGGAAGAAGGATGTAGCGGTTCAACTGCGTATAGGAAAAGATTTAATGACGAAACACTGTATCGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGGGAGAGACGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAAGAAGTGTATTTACGCCTTTCCACGCTTCCAACCGTATATCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAAAAAATACTCTCTTTTTTAC
ACTATTCACATGGGTTACTCGTATTTACTGGACCGACTGGTTCTGGCAAGACAACAACAATGTATGCATTATTAGAGGCG
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAATGATGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGACGGGATTAAAGGCTATTTTACGTCATGACCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACCGCAAAAATAGCTGTAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACATTGCAT
ACTAGTGACGCGAAAGGAGCGATACTACGATTTATGGATTATGGCATAACGAGGCAAGAGATTGAACAATCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGCCCGTTTTGCAGAGGAAACTGCTCTGTTTTATGTAAATCAATGCGGC
AAGTAAGGCAAGCGAGCATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGGAGCGAACGGAGAATGT
GTTACGTACAAGCATGAAACATTAGAATCTTCGTTACGTAAAGGATACGCCTTAGGATTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1A9PWS3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.772

100

0.568

Multiple sequence alignment