Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BTI247_RS21965 Genome accession   NZ_CP015250
Coordinates   4330485..4331528 (-) Length   347 a.a.
NCBI ID   WP_053563650.1    Uniprot ID   A0A9W3XAM4
Organism   Bacillus thuringiensis Bt18247     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4325485..4336528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTI247_RS21920 (BTI247_45070) - 4325919..4326119 (-) 201 WP_000106083.1 YqzE family protein -
  BTI247_RS21925 (BTI247_45080) - 4326158..4326655 (-) 498 WP_000836660.1 shikimate kinase -
  BTI247_RS21930 (BTI247_45090) - 4326771..4327421 (-) 651 WP_000183863.1 2OG-Fe(II) oxygenase -
  BTI247_RS21935 (BTI247_45100) comGG 4327596..4327967 (-) 372 WP_069356197.1 competence type IV pilus minor pilin ComGG -
  BTI247_RS21940 (BTI247_45110) comGF 4327964..4328433 (-) 470 Protein_4363 competence type IV pilus minor pilin ComGF -
  BTI247_RS21945 (BTI247_45120) - 4328403..4328705 (-) 303 WP_069356198.1 competence protein ComG -
  BTI247_RS21950 (BTI247_45130) comGD 4328698..4329153 (-) 456 WP_002094140.1 competence type IV pilus minor pilin ComGD -
  BTI247_RS21955 (BTI247_45140) comGC 4329150..4329449 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  BTI247_RS21960 (BTI247_45150) comGB 4329461..4330492 (-) 1032 WP_084746220.1 competence type IV pilus assembly protein ComGB -
  BTI247_RS21965 (BTI247_45160) comGA 4330485..4331528 (-) 1044 WP_053563650.1 competence type IV pilus ATPase ComGA Machinery gene
  BTI247_RS21970 (BTI247_45170) - 4331734..4332429 (+) 696 WP_000434107.1 helix-turn-helix domain-containing protein -
  BTI247_RS21975 (BTI247_45180) - 4332556..4332798 (+) 243 WP_000375619.1 DUF2626 domain-containing protein -
  BTI247_RS21980 (BTI247_45190) - 4332906..4334300 (+) 1395 WP_001094344.1 L-cystine transporter -
  BTI247_RS21985 (BTI247_45210) - 4334380..4334761 (-) 382 Protein_4372 hypothetical protein -
  BTI247_RS21990 (BTI247_45220) - 4334849..4335064 (-) 216 WP_001008326.1 DUF3912 family protein -
  BTI247_RS21995 (BTI247_45230) - 4335318..4335629 (+) 312 WP_001093240.1 hypothetical protein -
  BTI247_RS22000 (BTI247_45240) - 4335664..4336152 (-) 489 WP_000764263.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39178.54 Da        Isoelectric Point: 8.5432

>NTDB_id=178084 BTI247_RS21965 WP_053563650.1 4330485..4331528(-) (comGA) [Bacillus thuringiensis Bt18247]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRVGKDLMTRHCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=178084 BTI247_RS21965 WP_053563650.1 4330485..4331528(-) (comGA) [Bacillus thuringiensis Bt18247]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTGTAGGAAAAGATTTAATGACGAGACATTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTTAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTGCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTCATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCTAGCATTTATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCATCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.196

100

0.562
  pilB Haemophilus influenzae 86-028NP

39.441

92.795

0.366

Multiple sequence alignment