Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   A4265_RS06280 Genome accession   NZ_CP015238
Coordinates   1246356..1247681 (-) Length   441 a.a.
NCBI ID   WP_063632029.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NS53     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1241356..1252681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4265_RS06270 (A4265_05935) hpf 1245091..1245639 (-) 549 WP_002988974.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  A4265_RS06275 (A4265_05940) - 1245719..1246384 (-) 666 WP_063632027.1 ComF family protein -
  A4265_RS06280 (A4265_05945) comFA/cflA 1246356..1247681 (-) 1326 WP_063632029.1 DEAD/DEAH box helicase Machinery gene
  A4265_RS06285 (A4265_05950) - 1247736..1248368 (+) 633 WP_002983676.1 YigZ family protein -
  A4265_RS06290 (A4265_05955) cysK 1248496..1249437 (+) 942 WP_011528853.1 cysteine synthase A -
  A4265_RS06295 (A4265_05960) - 1249455..1249832 (-) 378 WP_032464495.1 S1 RNA-binding domain-containing protein -
  A4265_RS06300 (A4265_05965) - 1249832..1251232 (-) 1401 WP_063632031.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  A4265_RS06305 (A4265_05970) - 1251269..1251910 (-) 642 WP_002983671.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49790.27 Da        Isoelectric Point: 10.0084

>NTDB_id=177911 A4265_RS06280 WP_063632029.1 1246356..1247681(-) (comFA/cflA) [Streptococcus pyogenes strain NS53]
MESIENSYGRLLLESQLPDSARQLAQPLKSVVISRGKMICQRCHYQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPP
MHFPKGNYLVWGGQLTAYQEMISQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFNLFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAI
LKELLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=177911 A4265_RS06280 WP_063632029.1 1246356..1247681(-) (comFA/cflA) [Streptococcus pyogenes strain NS53]
ATGGAGTCTATTGAAAATAGTTATGGGCGGTTGTTGCTAGAAAGCCAGTTACCAGATTCAGCAAGGCAACTTGCTCAACC
TTTAAAAAGTGTAGTAATATCGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAGTGGAGCCTATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAGATAAATTGCTCTATGCTATCCCACCT
ATGCATTTTCCAAAAGGTAATTATCTGGTTTGGGGCGGTCAACTAACTGCTTATCAAGAGATGATTTCCCAACAATTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTGCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCCAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTCTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTCTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGGCTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCTGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTAACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTAAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTCATAATAGGCCATTAGT
TATTCCAAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCCTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCTATCCCCTTTTAATTTTTCTACCCACTATTATAATGGCAGAATCTGTTACAGCAATC
TTAAAAGAGTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGGAAAAAAACGATTTTAATTACAACAACAATTCTAGAAAGGGGTGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAGAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTACTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(289-396)

DEAD/DEAH box helicase (DEAD)

(98-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

54.415

95.011

0.517
  comFA/cflA Streptococcus pneumoniae D39

54.415

95.011

0.517
  comFA/cflA Streptococcus pneumoniae R6

54.415

95.011

0.517
  comFA/cflA Streptococcus pneumoniae TIGR4

54.415

95.011

0.517
  comFA/cflA Streptococcus mitis NCTC 12261

53.081

95.692

0.508
  comFA/cflA Streptococcus mitis SK321

52.133

95.692

0.499
  comFA Lactococcus lactis subsp. cremoris KW2

47.368

90.476

0.429
  comFA Latilactobacillus sakei subsp. sakei 23K

39.851

91.61

0.365

Multiple sequence alignment