Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   A2J09_RS10650 Genome accession   NZ_CP015224
Coordinates   2188926..2189258 (-) Length   110 a.a.
NCBI ID   WP_012295159.1    Uniprot ID   -
Organism   Lysinibacillus sphaericus strain 2362     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2183926..2194258
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A2J09_RS10615 (A2J09_10610) gcvPA 2184063..2185409 (-) 1347 WP_012295153.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  A2J09_RS10620 (A2J09_10615) gcvT 2185435..2186538 (-) 1104 WP_012295154.1 glycine cleavage system aminomethyltransferase GcvT -
  A2J09_RS10625 (A2J09_10620) - 2187041..2187556 (-) 516 WP_031416947.1 shikimate kinase -
  A2J09_RS10635 (A2J09_10630) comGF 2187755..2188183 (-) 429 WP_012295156.1 competence type IV pilus minor pilin ComGF -
  A2J09_RS10640 (A2J09_10635) - 2188176..2188493 (-) 318 WP_012295157.1 hypothetical protein -
  A2J09_RS10645 (A2J09_10640) comGD 2188486..2188926 (-) 441 WP_012295158.1 competence type IV pilus minor pilin ComGD -
  A2J09_RS10650 (A2J09_10645) comGC 2188926..2189258 (-) 333 WP_012295159.1 competence type IV pilus major pilin ComGC Machinery gene
  A2J09_RS10655 (A2J09_10650) comGB 2189269..2190342 (-) 1074 WP_012295160.1 competence type IV pilus assembly protein ComGB -
  A2J09_RS10660 (A2J09_10655) comGA 2190317..2191336 (-) 1020 WP_012295161.1 competence type IV pilus ATPase ComGA -
  A2J09_RS10665 (A2J09_10660) - 2191665..2192375 (+) 711 WP_012295162.1 helix-turn-helix transcriptional regulator -
  A2J09_RS10670 (A2J09_10665) - 2192493..2192735 (+) 243 WP_004231035.1 DUF2626 family protein -
  A2J09_RS10675 (A2J09_10670) - 2192795..2193439 (-) 645 WP_012295163.1 MBL fold metallo-hydrolase -
  A2J09_RS10680 (A2J09_10675) - 2193608..2193787 (+) 180 WP_031416957.1 DUF2759 domain-containing protein -

Sequence


Protein


Download         Length: 110 a.a.        Molecular weight: 12302.40 Da        Isoelectric Point: 4.6258

>NTDB_id=177708 A2J09_RS10650 WP_012295159.1 2188926..2189258(-) (comGC) [Lysinibacillus sphaericus strain 2362]
MKQIKQQNGFTLIEMLIVLLIISILILITIPNVTKHFATIDEKGCAAYINMVQGQVEAYRVDFMTFPTLEDLVKEGYLKE
NETTCPNKEEIVITIKGEVRLANADAGTQG

Nucleotide


Download         Length: 333 bp        

>NTDB_id=177708 A2J09_RS10650 WP_012295159.1 2188926..2189258(-) (comGC) [Lysinibacillus sphaericus strain 2362]
ATGAAACAAATCAAACAACAAAATGGCTTTACTTTAATCGAAATGCTGATTGTTTTGTTAATTATATCAATCCTTATTTT
AATTACAATTCCAAATGTTACGAAGCACTTTGCTACGATTGATGAGAAGGGCTGTGCGGCCTATATTAATATGGTGCAGG
GGCAAGTAGAGGCCTATAGAGTGGATTTTATGACATTTCCGACCCTTGAGGATTTAGTGAAGGAGGGCTATTTGAAAGAA
AATGAAACAACTTGCCCTAATAAAGAAGAAATAGTCATTACGATTAAAGGAGAGGTACGCTTAGCAAATGCTGATGCAGG
TACTCAAGGATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

54.082

89.091

0.482

  comGC Staphylococcus aureus MW2

45.263

86.364

0.391

  comGC Staphylococcus aureus N315

45.263

86.364

0.391


Multiple sequence alignment