Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   A4A60_RS13210 Genome accession   NZ_CP015222
Coordinates   2471673..2472743 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis strain HRBS-10TDI13     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2466673..2477743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4A60_RS13165 (A4A60_13175) tapA 2467152..2467913 (-) 762 WP_014480251.1 amyloid fiber anchoring/assembly protein TapA -
  A4A60_RS13170 (A4A60_13180) yqzG 2468185..2468511 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  A4A60_RS13175 (A4A60_13185) spoIITA 2468553..2468732 (-) 180 WP_014480252.1 YqzE family protein -
  A4A60_RS13180 (A4A60_13190) comGG 2468803..2469177 (-) 375 WP_069837632.1 ComG operon protein ComGG Machinery gene
  A4A60_RS13185 (A4A60_13195) comGF 2469178..2469561 (-) 384 WP_029317913.1 ComG operon protein ComGF Machinery gene
  A4A60_RS13190 (A4A60_13200) comGE 2469587..2469934 (-) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  A4A60_RS13195 (A4A60_13205) comGD 2469918..2470349 (-) 432 WP_014480256.1 comG operon protein ComGD Machinery gene
  A4A60_RS13200 (A4A60_13210) comGC 2470339..2470635 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  A4A60_RS13205 (A4A60_13215) comGB 2470649..2471686 (-) 1038 WP_031600685.1 comG operon protein ComGB Machinery gene
  A4A60_RS13210 (A4A60_13220) comGA 2471673..2472743 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  A4A60_RS13220 (A4A60_13230) - 2472955..2473152 (-) 198 WP_014480259.1 CBS domain-containing protein -
  A4A60_RS13225 (A4A60_13235) corA 2473154..2474107 (-) 954 WP_014480260.1 magnesium transporter CorA -
  A4A60_RS13230 (A4A60_13240) yqhB 2474249..2475577 (+) 1329 WP_014480261.1 hemolysin family protein -
  A4A60_RS13235 (A4A60_13245) rsbRD 2475630..2476466 (-) 837 WP_014480262.1 RsbT co-antagonist protein RsbRD -
  A4A60_RS13245 (A4A60_13255) mgsR 2476690..2477070 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  A4A60_RS13250 (A4A60_13260) yqgY 2477302..2477547 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -
  A4A60_RS23035 - 2477548..2477688 (+) 141 WP_155118966.1 hypothetical protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=177663 A4A60_RS13210 WP_004399124.1 2471673..2472743(-) (comGA) [Bacillus subtilis strain HRBS-10TDI13]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=177663 A4A60_RS13210 WP_004399124.1 2471673..2472743(-) (comGA) [Bacillus subtilis strain HRBS-10TDI13]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAAGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATTGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGTCGCCAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGGAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment