Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   Bt4C1_RS20815 Genome accession   NZ_CP015176
Coordinates   4101333..4102376 (-) Length   347 a.a.
NCBI ID   WP_001013181.1    Uniprot ID   -
Organism   Bacillus thuringiensis serovar alesti strain BGSC 4C1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4096333..4107376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Bt4C1_RS20770 (Bt4C1_20775) - 4096766..4096966 (-) 201 WP_000106083.1 YqzE family protein -
  Bt4C1_RS20775 (Bt4C1_20780) aroK 4097005..4097502 (-) 498 WP_000836635.1 shikimate kinase AroK -
  Bt4C1_RS20780 (Bt4C1_20785) - 4097618..4098268 (-) 651 WP_000183866.1 2OG-Fe(II) oxygenase -
  Bt4C1_RS20785 (Bt4C1_20790) comGG 4098443..4098814 (-) 372 WP_001214886.1 competence type IV pilus minor pilin ComGG -
  Bt4C1_RS20790 (Bt4C1_20795) comGF 4098811..4099281 (-) 471 WP_000923110.1 competence type IV pilus minor pilin ComGF -
  Bt4C1_RS20795 (Bt4C1_20800) - 4099251..4099553 (-) 303 WP_044784479.1 hypothetical protein -
  Bt4C1_RS20800 (Bt4C1_20805) comGD 4099546..4100001 (-) 456 WP_002082168.1 competence type IV pilus minor pilin ComGD -
  Bt4C1_RS20805 (Bt4C1_20810) comGC 4099998..4100297 (-) 300 WP_001178680.1 competence type IV pilus major pilin ComGC -
  Bt4C1_RS20810 (Bt4C1_20815) comGB 4100309..4101346 (-) 1038 WP_044784585.1 competence type IV pilus assembly protein ComGB -
  Bt4C1_RS20815 (Bt4C1_20820) comGA 4101333..4102376 (-) 1044 WP_001013181.1 competence type IV pilus ATPase ComGA Machinery gene
  Bt4C1_RS20820 (Bt4C1_20825) - 4102582..4103277 (+) 696 WP_000434110.1 helix-turn-helix domain-containing protein -
  Bt4C1_RS20825 (Bt4C1_20830) - 4103404..4103646 (+) 243 WP_000375619.1 DUF2626 domain-containing protein -
  Bt4C1_RS20830 (Bt4C1_20835) - 4103754..4105148 (+) 1395 WP_063675679.1 L-cystine transporter -
  Bt4C1_RS20835 (Bt4C1_20840) - 4105228..4105620 (-) 393 WP_063675680.1 hypothetical protein -
  Bt4C1_RS20840 (Bt4C1_20845) - 4105708..4105923 (-) 216 WP_001008326.1 DUF3912 family protein -
  Bt4C1_RS20845 (Bt4C1_20850) - 4106177..4106488 (+) 312 WP_001093240.1 hypothetical protein -
  Bt4C1_RS20850 (Bt4C1_20855) - 4106523..4107011 (-) 489 WP_000764263.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39168.55 Da        Isoelectric Point: 8.7212

>NTDB_id=177322 Bt4C1_RS20815 WP_001013181.1 4101333..4102376(-) (comGA) [Bacillus thuringiensis serovar alesti strain BGSC 4C1]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEANGEC
VTYKHETLESSIRKGYALGFLEDDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=177322 Bt4C1_RS20815 WP_001013181.1 4101333..4102376(-) (comGA) [Bacillus thuringiensis serovar alesti strain BGSC 4C1]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAATGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTTAAATTTTTAGCATCTATGGATATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGTATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTTACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAATGTCCGTTTTGCAGAGGGAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGGTATGAGTTAAAACAAGCGCTTAAAGAAGCGAATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGGTTTTTAGAAGATGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369

Multiple sequence alignment