Detailed information NTG0176908

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: A4U85_RS14560
• Genome accession: NZ_CP015121
• Coordinates: 3063035..3064153 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain ab736
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3058035..3069153

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A4U85_RS14550 (A4U85_14430)	-	3061151..3061843 (-)	693	WP_001108520.1	YggS family pyridoxal phosphate-dependent enzyme	-
A4U85_RS14555 (A4U85_14435)	pilT	3061970..3063007 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
A4U85_RS14560 (A4U85_14440)	pilU	3063035..3064153 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
A4U85_RS14565 (A4U85_14445)	fur	3064223..3064660 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
A4U85_RS14570 (A4U85_14450)	-	3064772..3065170 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
A4U85_RS14575 (A4U85_14455)	-	3065225..3065548 (-)	324	WP_000442584.1	RnfH family protein	-
A4U85_RS14580 (A4U85_14460)	-	3065548..3066615 (-)	1068	WP_005144332.1	hypothetical protein	-
A4U85_RS14590 (A4U85_14470)	-	3066768..3067223 (-)	456	WP_000782976.1	bacteriohemerythrin	-
A4U85_RS14595 (A4U85_14475)	-	3067476..3068324 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=176908 A4U85_RS14560 WP_000347036.1 3063035..3064153(+) (pilU) [Acinetobacter baumannii strain ab736]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=176908 A4U85_RS14560 WP_000347036.1 3063035..3064153(+) (pilU) [Acinetobacter baumannii strain ab736]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392