Detailed information NTG0176810

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: A4W92_RS10465
• Genome accession: NZ_CP015117
• Coordinates: 2252276..2253148 (+)
• Length: 290 a.a.
• NCBI ID: WP_016852118.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain ATCC 27853
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2247276..2258148

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A4W92_RS10450 (A4W92_10450)	-	2248659..2249123 (-)	465	WP_031642065.1	pilin	-
A4W92_RS10455 (A4W92_10455)	pilB	2249354..2251054 (+)	1701	WP_003122078.1	type IV-A pilus assembly ATPase PilB	Machinery gene
A4W92_RS10460 (A4W92_10460)	pilC	2251058..2252275 (+)	1218	WP_003107299.1	type II secretion system F family protein	Machinery gene
A4W92_RS10465 (A4W92_10465)	pilD	2252276..2253148 (+)	873	WP_016852118.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
A4W92_RS10470 (A4W92_10470)	coaE	2253145..2253756 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
A4W92_RS10475 (A4W92_10475)	yacG	2253753..2253953 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
A4W92_RS10480 (A4W92_10480)	-	2253990..2254199 (-)	210	WP_003094660.1	hypothetical protein	-
A4W92_RS10485 (A4W92_10485)	-	2254305..2254994 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
A4W92_RS10490 (A4W92_10490)	-	2254991..2255461 (-)	471	WP_003094664.1	hypothetical protein	-
A4W92_RS10495 (A4W92_10495)	-	2255458..2255883 (-)	426	WP_003116252.1	GNAT family N-acetyltransferase	-
A4W92_RS10500 (A4W92_10500)	-	2256016..2256645 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
A4W92_RS10505 (A4W92_10505)	-	2256642..2257091 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
A4W92_RS10510 (A4W92_10510)	-	2257117..2257287 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31845.85 Da
• Isoelectric Point: 7.7068

>NTDB_id=176810 A4W92_RS10465 WP_016852118.1 2252276..2253148(+) (pilD) [Pseudomonas aeruginosa strain ATCC 27853]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLASYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=176810 A4W92_RS10465 WP_016852118.1 2252276..2253148(+) (pilD) [Pseudomonas aeruginosa strain ATCC 27853]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGCCAGCTACCTGGCGCTGGGCGGCAAGTGTTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATACTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	48.561	95.862	0.466