Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AC251_RS16475 Genome accession   NZ_CP015115
Coordinates   3480758..3482257 (+) Length   499 a.a.
NCBI ID   WP_064477487.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain EP1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3475758..3487257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AC251_RS16450 (AC251_15980) - 3476951..3477934 (+) 984 WP_016724265.1 KpsF/GutQ family sugar-phosphate isomerase -
  AC251_RS16455 (AC251_15985) - 3477973..3478560 (+) 588 WP_011000373.1 KdsC family phosphatase -
  AC251_RS16460 (AC251_15990) lptC 3478562..3479194 (+) 633 WP_011000372.1 LPS export ABC transporter periplasmic protein LptC -
  AC251_RS16465 (AC251_15995) lptA 3479263..3479838 (+) 576 WP_011000371.1 lipopolysaccharide transport periplasmic protein LptA -
  AC251_RS16470 (AC251_16000) lptB 3479872..3480660 (+) 789 WP_016724264.1 LPS export ABC transporter ATP-binding protein -
  AC251_RS16475 (AC251_16005) rpoN/rpoN1 3480758..3482257 (+) 1500 WP_064477487.1 RNA polymerase factor sigma-54 Machinery gene
  AC251_RS16480 (AC251_16010) hpf 3482309..3482662 (+) 354 WP_011000368.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AC251_RS16485 (AC251_16015) ptsN 3483011..3483466 (+) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AC251_RS16490 (AC251_16020) hprK 3483537..3484511 (+) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  AC251_RS16495 (AC251_16025) - 3484702..3485037 (+) 336 WP_020425146.1 PsiF family protein -
  AC251_RS16500 (AC251_16030) rapZ 3485131..3486021 (+) 891 WP_058907464.1 RNase adapter RapZ -
  AC251_RS16505 (AC251_16035) - 3486099..3486749 (+) 651 WP_016724261.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55257.58 Da        Isoelectric Point: 4.8064

>NTDB_id=176799 AC251_RS16475 WP_064477487.1 3480758..3482257(+) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain EP1]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAANGADHADTAEASYENGSDFDSDYGTERSDWSLDDFARKPQSDEDDRAPMQLREAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
TIVNQHLELLAARDYTRLKKALSVDDGELKAAHELIRSLAPFPGHTFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=176799 AC251_RS16475 WP_064477487.1 3480758..3482257(+) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain EP1]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCCCAGCATCTGGCGCTCACGCCACAACTGCAGCAGTCGATCCGGCTACTGCA
GTTGTCGACCATCGAGCTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTCGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAACGCCCTGCAGAGCAGCAGCGCCACCCCGCTGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCGCGGACCATGCCGACACGGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGAGCGGTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGAGGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAACTCGCGCCGCTAAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAGCTCGACGAATTGCAGGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGTGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCGGCCAAGCCGCTGGCGCTG
ACCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGTTGTCCGTCGATGATGG
CGAGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTTCCTGGCCACACCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCCGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGTGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCAATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

99.8

100

0.998

Multiple sequence alignment