Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AMD27_RS13460 Genome accession   NZ_CP015110
Coordinates   2817683..2818486 (-) Length   267 a.a.
NCBI ID   WP_067661437.1    Uniprot ID   A0A143G6D4
Organism   Acinetobacter sp. TGL-Y2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2812683..2823486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMD27_RS13440 (AMD27_13445) - 2813675..2814418 (-) 744 WP_067661429.1 YfgM family protein -
  AMD27_RS13445 (AMD27_13450) hisS 2814447..2815742 (-) 1296 WP_067661431.1 histidine--tRNA ligase -
  AMD27_RS13450 (AMD27_13455) ispG 2815746..2816867 (-) 1122 WP_067661433.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  AMD27_RS13455 (AMD27_13460) - 2816886..2817692 (-) 807 WP_067661435.1 helix-turn-helix domain-containing protein -
  AMD27_RS13460 (AMD27_13465) pilF 2817683..2818486 (-) 804 WP_067661437.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  AMD27_RS13465 (AMD27_13470) rlmN 2818531..2819766 (-) 1236 WP_067661439.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  AMD27_RS13470 (AMD27_13475) ndk 2819903..2820334 (-) 432 WP_067661441.1 nucleoside-diphosphate kinase -
  AMD27_RS13475 (AMD27_13480) iscX 2820443..2820643 (-) 201 WP_067661443.1 Fe-S cluster assembly protein IscX -
  AMD27_RS13480 (AMD27_13485) - 2820787..2821338 (-) 552 WP_067663026.1 phosphatase PAP2 family protein -
  AMD27_RS13485 (AMD27_13490) - 2821358..2822620 (-) 1263 WP_228140670.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 29927.04 Da        Isoelectric Point: 9.5925

>NTDB_id=176716 AMD27_RS13460 WP_067661437.1 2817683..2818486(-) (pilF) [Acinetobacter sp. TGL-Y2]
MYKKSVAVMALCLSALVLQGCQSTDSGTIVKKDPEKAAKVRTQMAAEYIRTGDLDAAKRALDQALAADSKDSTANMMMGV
LLQQEGSQISLDQADRYFSRAISLDPKNAQARNNYGTYLYQAKRYNDAIQQFSIAGATLGYEQRFRALENLGRIYLIQGD
VANAEKSFKQALQVNRESTISMIELAEIFYLQQNHVAARQLYQSFVGNVGQKNQGARALWIGLRIARANGDNMGMHVLVN
QLRALFPNSQEYQRYLQLQYNTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=176716 AMD27_RS13460 WP_067661437.1 2817683..2818486(-) (pilF) [Acinetobacter sp. TGL-Y2]
ATGTATAAAAAATCAGTCGCTGTGATGGCACTGTGTTTGTCTGCATTGGTGTTACAAGGATGCCAGAGTACAGACTCAGG
CACGATTGTTAAAAAAGACCCTGAAAAGGCAGCCAAAGTTCGAACACAAATGGCTGCTGAATATATTCGCACAGGTGATT
TAGATGCCGCCAAGCGCGCATTGGATCAAGCCTTAGCCGCAGATTCAAAAGATTCAACGGCCAATATGATGATGGGCGTT
TTACTCCAACAAGAAGGCAGTCAAATTAGTTTAGATCAGGCAGATCGCTATTTTAGTCGTGCCATTTCACTTGATCCAAA
AAATGCGCAAGCGCGTAATAATTATGGAACATATTTATATCAAGCAAAACGTTATAATGATGCCATTCAACAGTTCAGTA
TTGCCGGTGCAACACTGGGCTATGAGCAGCGTTTTCGAGCTTTAGAAAATTTAGGTCGTATTTACCTGATTCAAGGTGAT
GTGGCGAATGCTGAAAAATCATTCAAACAGGCACTACAAGTCAATCGTGAATCGACCATTTCAATGATAGAGTTGGCTGA
GATTTTTTATCTGCAACAAAATCATGTGGCAGCGCGTCAACTTTACCAATCTTTTGTTGGTAATGTCGGCCAAAAAAATC
AAGGTGCGAGAGCGCTATGGATTGGTTTACGTATTGCGCGTGCCAATGGGGATAATATGGGAATGCATGTACTTGTGAAT
CAATTGCGTGCTTTGTTTCCAAATAGTCAAGAATATCAACGTTATTTGCAATTACAGTACAATACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(98-130)

Tetratricopeptide repeat (TPR_16)

(150-208)

Tetratricopeptide repeat (TPR_16)

(45-80)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A143G6D4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

73.077

97.378

0.712
  pilF Acinetobacter baylyi ADP1

67.442

96.629

0.652

Multiple sequence alignment